干细胞之家 - 中国干细胞行业门户第一站

 

 

搜索
干细胞之家 - 中国干细胞行业门户第一站 干细胞之家论坛 干细胞行业新闻 Hit-and-Run Action of Stem Cells Exploited for Targe ...
朗日生物

免疫细胞治疗专区

欢迎关注干细胞微信公众号

  
查看: 24264|回复: 0
go

Hit-and-Run Action of Stem Cells Exploited for Targeted Drug Delivery [复制链接]

Rank: 3Rank: 3

积分
385 
威望
385  
包包
1381  

优秀会员 金话筒

楼主
发表于 2013-10-9 09:13 |只看该作者 |倒序浏览 |打印
Scientists have inserted mRNA into mesenchymal stem cells (MSCs) to produce a drug-delivery vehicle. Following systemic administration, the modified MSCs targeted and adhered to sites of inflammation, then released interleukin-10 that significantly reduced local swelling.
  f& q0 ~% n  i, ?/ k0 r/ Q8 \* ^) n
Historically, MSC-based treatments have had mixed results. MSCs exert their therapeutic effects in hit-and-run style. That is, MSCs are rapidly cleared after entering the bloodstream, typically within a few hours or days. Yet, despite the transience of MSC therapeutic action, a team of scientists reports that it has engineered MSCs that rapidly localized at a distant site of inflammation in an in vivo model, and delivered therapeutically relevant concentrations of the drug. The MSCs had been engineered enhanced homing and the expression of interleukin-10, which is not inherently produced by MSCs.
$ O0 `# u; D+ v8 X8 t( R! w( z: l; [0 I  Q% m, t5 U
The team of scientists included members representing Brigham and Women’s Hospital, the Harvard Stem Cell Institute, MIT, and Massachusetts General Hospital. The team, which published the results of its proof-of concept study in the October 3 issue of Blood, notes that its work is already drawing interest from biopharmaceutical companies.) D; ^% ?- b9 T

* L8 L" X) Z& J* a) O“This opens the door to thinking of mRNA transfection of cell populations as next-generation therapeutics in the clinic, as they get around some of the delivery challenges that have been encountered with biological agents,” said Oren Levy, Ph.D., co-lead author of the study and instructor of medicine in the laboratory of research leader Jeffrey Karp, Ph.D. Dr. Karp, a Harvard Stem Cell Institute principal faculty member and associate professor at the Brigham and Women's Hospital, is also affiliated with Harvard Medical School and MIT.3 i% G; ~) |6 c. X* z5 G2 a

- P$ f# h9 t' N9 K4 @. j/ u“If you think of a cell as a drug factory, what we’re doing is targeting cell-based, drug factories to damaged or diseased tissues, where the cells can produce drugs at high enough levels to have a therapeutic effect,” said Dr. Karp.4 I+ Z4 h, {3 o" A5 z

+ I0 n  x) Y; {! o* }2 E9 ~% TThe key to success was the use of triple-transfected MSCs. In addition to expressing interleukin-1-, the cells expressed, in the team’s words, “functional rolling machinery.” This machinery consisted of P-selectin glycoprotein ligand-1(PSGL-1) and Sialyl-Lewisx (SLeX). Basically, the cells expressed both the immunomodulating agent and adhesive surface proteins.
/ C9 X  _: N6 k) c5 R' I
已有 1 人评分威望 包包 收起 理由
细胞海洋 + 2 + 10 极好资料

总评分: 威望 + 2  包包 + 10   查看全部评分

‹ 上一主题|下一主题
你需要登录后才可以回帖 登录 | 注册
验证问答 换一个

Archiver|干细胞之家 ( 吉ICP备2021004615号-3 )

GMT+8, 2025-5-29 10:36

Powered by Discuz! X1.5

© 2001-2010 Comsenz Inc.