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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑 # K; I' l- j; D7 E! V. p$ J
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《神经生物学实验操作手册》目录) I% ?; H* N( m6 k
简介 * u- y9 G, X0 ]& e5 S0 m) G4 n
神经干细胞及神经发育 ······································································56 C) t+ c! e2 V/ B6 V( V
神经系统疾病中的神经细胞类型 ···························································8$ }9 m1 _6 I0 u2 n- Z- `- n: \
神经细胞培养及分化
7 n) w3 A$ h9 C1 |7 @2 I 人神经干细胞培养 ············································································ 11
! j* Y& N7 T; Z( K: y2 U 大鼠胚胎神经干细胞培养 ····································································15" D9 s1 X, N. A1 P( u3 C% e9 O
神经干细胞的无外源成分培养 ·······························································19/ H$ J. [, e4 o1 p5 ~2 p
人多能干细胞诱导成神经干细胞 ····························································21
% \ B% c& k% m! Q 神经干细胞分化为神经元和神经胶质细胞 ················································318 G$ r5 {$ C) C5 }5 ?" i: B7 L
神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
0 ]" b4 I* v$ a3 ~- l# H9 I 皮层星形胶质细胞的诱导和培养 ···························································41
; T: h2 f. e1 u) I* l) m 皮层及海马神经元的分离、培养和鉴定 ··················································43 b. F' \2 D: B: j
多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································480 K* v7 w* {: V. V+ C
多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································567 `6 e! Q' w4 W3 V( e; `
神经干细胞的冻存 ············································································606 H& ^) Q) e! a: C8 d: W. J
冻存及复苏成熟分化的神经细胞 ···························································62( o3 C5 m/ z. `) a- V- L
细胞分析
8 F3 q! f- f- v! I 神经干细胞的细胞活性分析 ·································································64
+ `( b1 p+ ^/ V+ d { 神经亚型特性标志 ············································································66
3 n- D" u8 K9 I3 R5 i 表面标志流式细胞分析 ······································································67
# l+ s% a$ B; ~* o免疫细胞化学分析 ···············································································695 P2 ?4 Q0 i q& M: m
使用Fluo-4 进行细胞内钙流检测 ······························································72$ k/ R0 M% g+ ^
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发表于 2013-10-18 13:08
发表于 2013-10-18 13:57
