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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑 5 n3 k4 Q! Q% D+ E" j l/ D2 k
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! K3 h+ S4 }2 ]三种方式免费获取《神经生物学实验操作手册》(中文精选版)
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——手把手教您从零开始神经细胞的分离、培养和鉴定
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) I j5 m' Y0 O. x }, T) m) \《神经生物学实验操作手册》目录
/ s$ W! Q& \' A! u" o1 A简介
$ L0 ?: }5 ]' z7 M7 j7 C) \ 神经干细胞及神经发育 ······································································5
- r- M: M( D, a: u$ Q: z 神经系统疾病中的神经细胞类型 ···························································8
! Z3 I5 ?- I; M3 n1 R神经细胞培养及分化 1 T8 W0 O$ m& ~9 k3 a+ `% x
人神经干细胞培养 ············································································ 11
" i' F' i" F* V( B, D& p 大鼠胚胎神经干细胞培养 ····································································15- J- n) X7 g7 i0 Q. g
神经干细胞的无外源成分培养 ·······························································19
' z6 D# f4 I; G& s/ B I 人多能干细胞诱导成神经干细胞 ····························································21
; J7 f8 o! G7 S0 C' K4 A9 Z K 神经干细胞分化为神经元和神经胶质细胞 ················································31
( Q& |* }) C. [! {9 h( h 神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
3 t3 D/ Z1 s N% {- t5 o3 } 皮层星形胶质细胞的诱导和培养 ···························································41; k, S& [8 `$ U' p1 z' s8 S. w
皮层及海马神经元的分离、培养和鉴定 ··················································433 S) K. v& ~( m
多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································48
! B% H6 g; ]5 Y 多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································56: s j- X6 W0 x1 R" s
神经干细胞的冻存 ············································································605 ]) E. O g: ~" Z
冻存及复苏成熟分化的神经细胞 ···························································62
% D- G* C" O+ H8 Y+ G6 B细胞分析
, a \% |' f- j { @; M 神经干细胞的细胞活性分析 ·································································64
* H: h" v7 q% d, Z8 P 神经亚型特性标志 ············································································662 N+ j2 w. E6 l
表面标志流式细胞分析 ······································································67
" M. T! t! O" J( A% j2 Y免疫细胞化学分析 ···············································································69; K- j( i/ N% S9 P
使用Fluo-4 进行细胞内钙流检测 ······························································720 ]' @' L4 E1 F0 J1 K' k2 F o' k1 ]$ R0 R
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发表于 2013-10-18 13:08
发表于 2013-12-3 13:52
书已经收到，赞一个。
能不能给我也发一份7 I0 Z9 D- K; w ]( t# }
