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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑 & w, h( [, E2 R$ ^9 I
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《神经生物学实验操作手册》目录
2 `3 _ j" s) e: t. B简介
7 }+ a1 ~# z7 p6 p z E+ G! {: C! { 神经干细胞及神经发育 ······································································55 c. h& m1 u8 V- `% r1 A
神经系统疾病中的神经细胞类型 ···························································8) m, N( g% `( R3 o$ Z' m
神经细胞培养及分化 $ t8 J# k8 I, g D+ @! z
人神经干细胞培养 ············································································ 11
- G0 U+ f; v; Y4 c 大鼠胚胎神经干细胞培养 ····································································15
. D7 K7 t4 |* p" b0 X" m 神经干细胞的无外源成分培养 ·······························································19$ o5 c( M \ j' M* S% u9 j
人多能干细胞诱导成神经干细胞 ····························································216 v) G# A0 P- f
神经干细胞分化为神经元和神经胶质细胞 ················································31- K1 A" E0 Q% L! q; v# [6 P
神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
8 ^6 ^0 T v7 R/ F5 G 皮层星形胶质细胞的诱导和培养 ···························································41
1 v% V/ ]9 a0 `" Z' a 皮层及海马神经元的分离、培养和鉴定 ··················································43
/ t9 M+ S. y4 l1 H9 p! ^ 多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································482 a6 X% i8 Z6 C
多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································56
& g! \: d3 W% F# K" S9 p 神经干细胞的冻存 ············································································60* c1 m% g2 r3 w; t' i" m: c' @+ U6 U
冻存及复苏成熟分化的神经细胞 ···························································623 w; B$ O4 I, a+ d5 x7 F
细胞分析
6 K5 i! W7 W/ v+ U0 D 神经干细胞的细胞活性分析 ·································································64/ H4 Q: X+ @) S" y- m. I$ K
神经亚型特性标志 ············································································66
$ D8 d& E& b/ i. g 表面标志流式细胞分析 ······································································679 |" c2 D0 {$ J! I% }
免疫细胞化学分析 ···············································································69
9 G, q* ?, ?0 R1 G% I使用Fluo-4 进行细胞内钙流检测 ······························································72
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发表于 2013-10-18 13:08
发表于 2013-10-18 13:57
