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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑
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《神经生物学实验操作手册》目录* c4 L/ `0 f6 P7 v! T, c" e) n2 S
简介
' ^# H5 G5 ^+ o4 M" G 神经干细胞及神经发育 ······································································5% G- n0 l4 e. m$ D, ^ E
神经系统疾病中的神经细胞类型 ···························································8
' F! F) E# ]! e神经细胞培养及分化
7 x: ?+ `* I8 r+ m* ^6 f: ] P 人神经干细胞培养 ············································································ 11
8 v" M+ G3 I9 m, L 大鼠胚胎神经干细胞培养 ····································································158 a* F/ L, B: ^9 H( I+ L
神经干细胞的无外源成分培养 ·······························································19
+ H6 @5 Z5 U2 @- t1 t+ s 人多能干细胞诱导成神经干细胞 ····························································21 _" w$ V. k8 w( W! @1 Y: Z
神经干细胞分化为神经元和神经胶质细胞 ················································31+ y9 t6 w/ a9 U* {% O
神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························386 u( v" f9 B' }% [! o( H, T
皮层星形胶质细胞的诱导和培养 ···························································413 _. P6 ? U9 d
皮层及海马神经元的分离、培养和鉴定 ··················································43
/ @7 J8 Z1 F, [* B! _6 x9 b% ? 多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································487 E) R# X& ?1 R/ r3 N. X9 C& U; D
多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································56
: ?8 n; `7 `* s6 w# D* S 神经干细胞的冻存 ············································································60' K3 ]2 b; Y! C" W. P
冻存及复苏成熟分化的神经细胞 ···························································62$ A5 x: B; U5 M. i: j' W% C) N
细胞分析
0 A, Z* ]% i% Q& x, U# F- q 神经干细胞的细胞活性分析 ·································································64
- w7 H, a, i/ J0 F 神经亚型特性标志 ············································································66
# X. @$ O5 @1 G& u) \ 表面标志流式细胞分析 ······································································67
0 M+ |9 `4 y- w2 V免疫细胞化学分析 ···············································································69* O x' T. P, K
使用Fluo-4 进行细胞内钙流检测 ······························································72
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发表于 2013-10-18 13:08
发表于 2013-10-18 13:57
