新仪器可高效率的将干细胞移植入跳动心脏中

海港椰树

新仪器可高效率的将干细胞移植入跳动心脏中7 v$ o: k/ X9 y4 d2 z, w2 L
录入：佚名    文章来源：生物谷       更新时间：2013-12-19
$ W7 [% @# W0 T- a* f/ U　　梅奥诊所的科学家今日开发出专门的导管可以将干细胞移植到跳动心脏中。该仪器能够最大限度的转移干细胞来修复心脏，相关报道发表在近期的Circulation: Cardiovascular Interventions杂志上。1 t# m0 r! S5 J$ o. e% k; U

0 b% w8 x$ j& z　　梅奥诊所心脏病学家Atta Behfar博士称，尽管生物治疗越来越普遍，但是再生疗法中将干细胞移植到心脏中的工具还具有缺陷。这些工具不能让足够量的干细胞到达心脏。干细胞的保留程度显然是有效治疗的关键。
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8 Q% |/ `8 K4 q, ?　　该新仪器具有一个弯曲的针，和沿着针走向逐渐变大的开口。该仪器的有点包括：
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/ t( n! c  s' j2 P) w　　1,弯曲形导管减少了回流，就会减少细胞损失。
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! d3 q" E5 B' j- S　　2,侧面逐渐从小变大的孔减小了对心脏的压力，保持细胞的靶向性。6 @8 s3 D$ ?' p8 w) |9 R

' D) h: o; I) [: I0 Z　　3,该仪器对健康心脏和受损心脏都有效。
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( `) Q% g8 m5 R, _; h" C; I/ D　　该新导管已经在欧洲进行临床试验。
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　　Optimized Delivery System Achieves Enhanced Endomyocardial Stem Cell Retention+ d# j" u6 R. m- |* F9 O. c
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　　Atta Behfar, Jean-Pierre Latere, Jozef Bartunek, Christian Homsy, Dorothee Daro, Ruben J. Crespo-Diaz, Paul G. Stalboerger, Valerie Steenwinckel, Aymeric Seron, Margaret M. Redfield, and Andre Terzic1 X  A: O1 U9 |/ G
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　　Background—Regenerative cell-based therapies are associated with limited myocardial retention of delivered stem cells. The objective of this study is to develop an endocardial delivery system for enhanced cell retention.% N4 P% u, T$ g# F
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　　Methods and Results—Stem cell retention was simulated in silico using 1- and 3-dimensional models of tissue distortion and compliance associated with delivery. Needle designs, predicted to be optimal, were accordingly engineered using nitinol, a nickel and titanium alloy displaying shape memory and superelasticity. Biocompatibility was tested with human mesenchymal stem cells. Experimental validation was performed with species-matched cells directly delivered into Langendorff-perfused porcine hearts or administered percutaneously into the endocardium of infarcted pigs. Cell retention was quantified by flow cytometry and real-time quantitative polymerase chain reaction methodology. Models, computing optimal distribution of distortion calibrated to favor tissue compliance, predicted that a 75°-curved needle featuring small-to-large graded side holes would ensure the highest cell retention profile. In isolated hearts, the nitinol curved needle catheter (C-Cath) design ensured 3-fold superior stem cell retention compared with a standard needle. In the setting of chronic infarction, percutaneous delivery of stem cells with C-Cath yielded a 37.7±7.1% versus 10.0±2.8% retention achieved with a traditional needle without effect on biocompatibility or safety.
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　　Conclusions—Modeling-guided development of a nitinol-based curved needle delivery system with incremental side holes achieved enhanced myocardial stem cell retention.