EB形成方法总结

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一、Heterogeneous methods5 R: m; b  S7 B+ J) J. L& E3 p2 G
       1. liquid suspension culture(LSC)
7 U- `/ O  C; r' x       2. stirred flask culture
4 D! G% Y) T4 M" \! k* _       3. rotary cell culture systems(RCCSS)7 t6 {6 _) j7 J) ^% Z/ q6 J) o
二、homogeneous methods
' a8 J6 _  J* T4 q# V5 H( V. Y       1. hanging drop culture
1 n9 S" [$ k2 d3 T* T       2. low adhesion U-bottom multiwell plates
* [+ a! u8 D7 Y" s       3. indented solid microsurfaces(Aggrewell TM)
( ]/ a3 Q. `9 T/ }$ ^* K1 z三、other methods$ y; ]& T! H. h6 \
       hydrogels(e.g., methylcellulose, agarose, alginate)4 z  c0 a2 \6 K- J2 m- p5 t

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