求教！怎么纯化间充质干细胞

ccwei0615

Have no experience on UC-MSC, but in my BM-MSC I always culture it in low density (100 cell/cm square). Since in my condition (hypoxia) MSCs' growth rate is faster than other cell types. After 3 passages, MSCs account for more than 95% in purity.

gghywtfz

流式筛一遍。去得干干净净2 j" v  x1 G. N) J) N