求教！怎么纯化间充质干细胞

ccwei0615

Have no experience on UC-MSC, but in my BM-MSC I always culture it in low density (100 cell/cm square). Since in my condition (hypoxia) MSCs' growth rate is faster than other cell types. After 3 passages, MSCs account for more than 95% in purity.

ccwei0615

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my incubator could detect O2 concentration, automatically refill N2 if the O2 is higher than 1%.