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本帖最后由 kakapo 于 2014-8-20 23:38 编辑
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: y U( h( P, q ?When growth factors in culture medium gets lower, the MSC possibly gets transformed. For the MSC culture, you can not wait until medium turned to yellow. When you seeded in 30% confluent, you have to pass the cells after three days. Because MSC in lower serum condition is going to differentiation or transformation. Maintaining the cells in stem cells potential, you should take care for this. |
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发表于 2014-8-20 23:36
