癌症的表型逆转问题

饶冠华

癌症的表型逆转问题一向是癌症研究人员最关心的问题。有那些方式可以逆转癌症表型呢？
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; s. X/ L/ V) ]( ~外周环境可以逆转部分癌症细胞的表型, O7 v5 x3 c& ?
1. Mintz B, Illmensee K.Normal genetically mosaic mice produced from malignant teratocarcinoma cells [J].Proc Natl Acad Sci U S A. 1975, 72(9):3585-9.
# _9 c- u; P2 N9 G2. Illmensee K, Mintz B.Totipotency and normal differentiation of single teratocarcinoma cells cloned by injection into blastocysts [J]. Proc Natl Acad Sci U S A. 1976, 73(2):549-53.
/ a) n8 R, K, ?: E- \7 ~- M3 Rosen P. On the reversibility of tumor cells.Med Hypotheses. 1981 May;7(5):615-20.
( s) b# A2 B7 V9 w4) Kaiser HE. Biological viewpoints of neoplastic regression.In Vivo. 1994 Jan-Feb;8(1):155-65. Review. 5 O( c( V  w. n+ @) _1 |) Y
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5) Blelloch RH, Hochedlinger K, Yamada Y, Brennan C, Kim M, Mintz B, Chin L, Jaenisch R. Nuclear cloning of embryonal carcinoma cells.Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):13985-90. Epub 2004 Aug 11. Erratum in: Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14305.
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; v% V/ a. A. c5 t! L2 k6) Pierce GB, Arechaga J, Wells RS. Embryonic control of cancer.
4 {6 Z) w" H7 Y& Y! GProg Clin Biol Res. 1986;226:67-77. Review. . O' E) O3 E! y

5 Q# B6 ?$ K5 i, x7) Barrett JC. A National Institutes of Health Workshop Report. Cellular and molecular mechanisms for suppression and reversion of tumorigenicity. A Chemical Pathology Study Section workshop.Cancer Res. 1987 May 1;47(9):2514-20. 4 r5 H( r' _( E
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8): Nature. 1984 Jun 7-13;309(5968):552-6.
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Inability of Rous sarcoma virus to cause sarcomas in the avian embryo.
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Dolberg DS, Bissell MJ. 3 L. U1 `6 S' \1 h

4 |, H/ m4 V/ f1 ?  ~' CThe injection of Rous sarcoma virus (RSV) into the wing web of newly hatched chicks causes a rapidly growing sarcomatous tumour which is palpable within 1 week of inoculation; and cultures of fibroblasts derived from chick embryos (CEF) and infected with RSV become rapidly transformed. Genetic studies have determined that expression of a single viral gene, designated v-src, is necessary for neoplastic transformation. This gene codes for a 60,000-molecular weight phosphoprotein termed pp60SPC , which possesses a protein kinase activity that phosphorylates polypeptides on tyrosine residues and is constitutively expressed in infected CEF cells. It has been suggested that transformation, and possibly tumorigenesis, may result solely from the consequences of this increase in tyrosine phosphorylations. The pathogenicity of RSV in chick embryos in ovo is less clear. Murphy and Rous suggested that RSV may have caused tumours in "various tissues" of "some embryos", but the subsequent studies of Milford and Duran - Reynals , as well as several other laboratories, failed to find any evidence of intraembryonic tumours in RSV-infected early embryos. The findings of Duran - Reynals , if correct, cannot be explained easily in view of our present understanding of RSV tumorigenicity. Thus, we have re-examined the interaction of RSV with the avian embryo and confirm here that RSV is nontumorigenic and non-teratogenic when microinjected into day 4 chicken embryos. In addition, we found that (1) the virus not only replicates in the embryo, but it also expresses an active src-specific protein kinase and (2) once the cells from the infected limbs are disrupted and placed in culture, they are capable of expressing the transformed phenotype after a 24-h delay.9 F1 D9 ?0 |6 E. o, [& ]% D! J0 U
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阻断癌基因可以部分逆转癌症表型。1 A$ d$ x: q. B8 L8 n' _( F6 t

3 v3 j# Q$ E3 p$ r3 `1 v7 H1）Arvanitis C, Felsher DW. Conditional transgenic models define how MYC initiates and maintains tumorigenesis. Semin Cancer Biol. 2006 Aug;16(4):313-7. Epub 2006 Jul 21. Review. # I8 c' P/ s6 h- Y1 I  L
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2）Shachaf CM, Felsher DW. Rehabilitation of cancer through oncogene inactivation.8 ?* |0 `7 [7 U  }5 o- T
Trends Mol Med. 2005 Jul;11(7):316-21. Review.
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  ]- H4 L" W# {  P7 L0 O3）Felsher DW. Reversibility of oncogene-induced cancer.  l9 D7 i& L: y; ~  C
Curr Opin Genet Dev. 2004 Feb;14(1):37-42.
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4 j" j3 V  K/ \8 W. N, S细胞融合可以逆转癌细胞表型：
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1）Harris H. The analysis of malignancy by cell fusion: the position in 1988.
. d* J) t. f1 A' r2 pCancer Res. 1988 Jun 15;48(12):3302-6.
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( b  k2 x) G! v  T& J1 x0 u1 @" j2)Handmaker SD. Hybridization of eukaryotic cells.
( I1 C$ ~/ b% c+ z% `* wAnnu Rev Microbiol. 1973;27:189-204. Review.8 E! R' ?8 D& K+ j$ U6 v$ U
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器官再生能力与癌变概率相反，与逆转癌症概率成正比：
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1) Prehn RT. Regeneration versus neoplastic growth. Carcinogenesis. 1997 Aug;18:1439-44. 1 p" ^. n( I5 h8 D9 G3 J* _8 n: p, n- O

2 A' Z0 p9 d, ?/ F: D, [2)Prehn RT. Immunosurveillance, regeneration and oncogenesis.Prog Exp Tumor Res. 1971;14:1-24. Review.

xiaodongqin

很好

sunny

谢谢分享，找到家了

zhoujiejun

非常感谢

wzx6660

再回过头看看，还是有收获，谢谢