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- 积分
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- 威望
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- 89
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人胚胎干细胞向肝细胞诱导的方案：
: U# \/ U6 M# ~6 S1、定型内胚层诱导：RPMI 1640+B27+100ng/ml Activin+50ng/ml Wnt3a培养3天2 S8 J P+ z' q
2、向肝细胞诱导：KO-DMEM+20%SR+1%DMSO 培养4天* V) L% _8 n. |2 t0 p c, `$ i& E
3、肝细胞成熟：L-15+10%FBS+10ng/ml HGF+20ng/ml OSM培养7天& D9 `; p- T; A7 o
" f4 K% {3 X: f( @$ ?- X+ ?参考文献：Highly efficient differentiation of hESCs to functional hepaic endoderm requires ActivinA and Wnt3a signaling. PNAS;2008 |
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总评分: 包包 + 6
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发表于 2009-3-20 11:05
