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人胚胎干细胞向肝细胞诱导的方案:
+ O& b& c1 a! h" i1、定型内胚层诱导:RPMI 1640+B27+100ng/ml Activin+50ng/ml Wnt3a培养3天& q/ g1 w: T; S z
2、向肝细胞诱导:KO-DMEM+20%SR+1%DMSO 培养4天
0 _ v& g6 ]/ @* r! f0 `/ P3、肝细胞成熟:L-15+10%FBS+10ng/ml HGF+20ng/ml OSM培养7天 W; }3 y; m: m" a1 J/ i, H. z+ h
0 ?4 q8 v3 [1 G3 L$ O* t- L+ y
参考文献:Highly efficient differentiation of hESCs to functional hepaic endoderm requires ActivinA and Wnt3a signaling. PNAS;2008 |
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