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人胚胎干细胞向肝细胞诱导的方案:
5 @8 ]! x: f. c% K4 o* }1、定型内胚层诱导:RPMI 1640+B27+100ng/ml Activin+50ng/ml Wnt3a培养3天
' G( I+ a2 j4 Y2、向肝细胞诱导:KO-DMEM+20%SR+1%DMSO 培养4天1 x7 d7 S2 J8 ^$ ?8 e9 K% \: c$ e
3、肝细胞成熟:L-15+10%FBS+10ng/ml HGF+20ng/ml OSM培养7天, c, `. e/ u$ Y& V; \+ n: j
2 y& ]" z* k+ u参考文献:Highly efficient differentiation of hESCs to functional hepaic endoderm requires ActivinA and Wnt3a signaling. PNAS;2008 |
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