求教paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

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Carboxyfl uorescein succinimidyl ester (CFSE;
- Q' q* i7 p2 K1 {) zInvitrogen)-labeled PBMC were cultured at3 d2 \5 t' W8 G4 H  Q
400 000 cells/well in a 24-well plate with cultured,1 ~! A: V( r& j
freshly thawed or paraformaldehyde (PFA)-fi xed
' ]# ?/ l  C; K5 wMSC at a ratio of MSC:PBMC of 1:3 and 1:9. T8 }7 x/ F: c: E8 C2 S
lymphocytes were stimulated using 0.4 μ g/mL antihuman
1 Q+ v  |" l, C  g, ACD3 and CD28 antibodies (clone HIT3a7 }+ L9 U8 _- I: c: D
and CD28.2, respectively, from BD Pharmagen,
: h+ d% [8 W1 ]' l' [Mississauga, ON, Canada). T-cell proliferation was( z7 F; E" G) y
determined 4 days later by fl ow cytometry analysis
2 U5 q# a& L% C: d: ?% Kof CFSE fl uorescence intensity.
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paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

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回复 cleargreat 的帖子, p/ M& V: l* R2 g7 n

# L. e4 g/ l9 L1 u" e3 [+ g4 N, e/ N如果用丝裂霉素处理PBMC的话，怎么研究MSC对PBMC的增值抑制作用

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回复 cloudycity 的帖子
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MSC为何要用PFA固定呢？