求教paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

未来之星

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Carboxyfl uorescein succinimidyl ester (CFSE;
7 X9 Z& s! l  s: z+ g+ `8 r  ZInvitrogen)-labeled PBMC were cultured at4 H% B5 G& ?, o+ o( {
400 000 cells/well in a 24-well plate with cultured,; o+ A1 C/ x, ?: |
freshly thawed or paraformaldehyde (PFA)-fi xed- Y0 c, K' [0 N$ R+ G# ]0 |
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CD3 and CD28 antibodies (clone HIT3a
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of CFSE fl uorescence intensity.
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paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

cleargreat

丝裂霉素是处理PBMC的吧。

未来之星

回复 cleargreat 的帖子
* S) q9 e2 ~8 n9 @, W% }* s3 ~
) _! A- M2 {+ m6 |0 z! V如果用丝裂霉素处理PBMC的话，怎么研究MSC对PBMC的增值抑制作用

cloudycity

这说的是PFA固定过的MSC吧···$ r/ M8 f/ T0 Q. ?
但是一般灭活都是用丝裂霉素或是射线灭活，PFA还真没用过···

未来之星

回复 cloudycity 的帖子0 s: n/ S5 i- D- ]! g

+ t# c! W1 J* F* s- m. UMSC为何要用PFA固定呢？