求教paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

未来之星

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Carboxyfl uorescein succinimidyl ester (CFSE;5 \# b& f& y, L& q' `/ {: \
Invitrogen)-labeled PBMC were cultured at3 C! a" {- J: z& m) m
400 000 cells/well in a 24-well plate with cultured,& v, G6 p; M6 G8 k% c1 `- s
freshly thawed or paraformaldehyde (PFA)-fi xed' H6 N9 Q! p; S' G
MSC at a ratio of MSC:PBMC of 1:3 and 1:9. T9 g) W/ p2 d6 w; q$ |
lymphocytes were stimulated using 0.4 μ g/mL antihuman
2 a4 R" J5 p- W2 E3 E( d; t+ ^& xCD3 and CD28 antibodies (clone HIT3a2 u7 J# X8 }: e) N( {
and CD28.2, respectively, from BD Pharmagen,
! |# p  t. S9 \/ e5 ]Mississauga, ON, Canada). T-cell proliferation was* `  ]  W$ o$ L( }( x9 I
determined 4 days later by fl ow cytometry analysis
7 _4 ]: P" h) c# x, Cof CFSE fl uorescence intensity.
( I; v8 k/ P- m  t& X0 r0 H$ K& d: H) I) E4 u- z
paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

cleargreat

丝裂霉素是处理PBMC的吧。

未来之星

回复 cleargreat 的帖子$ P" g6 ^/ G1 D+ D: H
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如果用丝裂霉素处理PBMC的话，怎么研究MSC对PBMC的增值抑制作用

cloudycity

这说的是PFA固定过的MSC吧···
2 Y9 c& |' t( u7 t/ M' p/ `0 F但是一般灭活都是用丝裂霉素或是射线灭活，PFA还真没用过···

未来之星

回复 cloudycity 的帖子# N! t% ^* F+ [% Q6 z+ B2 t% o

' P* W  v. a. t; n$ ]. {9 l7 ?MSC为何要用PFA固定呢？