求教paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

未来之星

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Carboxyfl uorescein succinimidyl ester (CFSE;8 z0 U! }% T* K6 p3 r# O9 Y+ Q
Invitrogen)-labeled PBMC were cultured at
# ?! |& O( ^- Q400 000 cells/well in a 24-well plate with cultured,
0 M! K8 L+ L7 B/ i# d2 H$ bfreshly thawed or paraformaldehyde (PFA)-fi xed# ], Z& V+ k5 a1 D3 r
MSC at a ratio of MSC:PBMC of 1:3 and 1:9. T
: y. I8 p# F% B0 c, ?lymphocytes were stimulated using 0.4 μ g/mL antihuman
' e2 c$ R+ L' Q9 k7 Y- vCD3 and CD28 antibodies (clone HIT3a
" `2 C" c% G( eand CD28.2, respectively, from BD Pharmagen,# I+ Q. A5 Y" v& k4 l
Mississauga, ON, Canada). T-cell proliferation was
" Y2 k; e5 }; z) N: h% [determined 4 days later by fl ow cytometry analysis9 `! t4 Z% r! M  @0 n! J8 k' g
of CFSE fl uorescence intensity./ p5 \( h' v% X8 g0 b6 G
4 K2 P( y! ?9 ]% k9 T: E
paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

cleargreat

丝裂霉素是处理PBMC的吧。

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回复 cleargreat 的帖子$ i2 e7 C* |) U. m8 C2 j5 J
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如果用丝裂霉素处理PBMC的话，怎么研究MSC对PBMC的增值抑制作用

cloudycity

这说的是PFA固定过的MSC吧···
) [2 }, F  ]0 \7 a" U5 H但是一般灭活都是用丝裂霉素或是射线灭活，PFA还真没用过···

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  v5 g6 T1 G. C: P0 FMSC为何要用PFA固定呢？