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本帖最后由 细胞海洋 于 2015-9-17 12:45 编辑 8 u9 c2 u$ } X; k
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3 a% ~1 J0 b! |+ [) w* ~Contents
% |- p, M8 O4 B p0 q0 zPreface............................................................. v
# C$ T6 a8 G2 `/ M3 wContributors......................................................... xi6 r, r( t3 w) c- G$ M! O3 n; ~
1 Cancer Biology ................................................... 1
6 P0 y' F8 V* H" ~9 a' a, CIan A. Cree
' x: O' P7 R7 A 2 Principles of Cancer Cell Culture ..................................... 13: o9 W* d' |- J
Ian A. Cree 2 @% e! n3 k$ s
3 Storage of Cell Lines............................................... 27
" Q; A1 x, G- P0 }% n% L: T! bKatharine A. Parker - u$ T( m$ Z% J# [( V
4 Characterization and Authentication of Cancer Cell Lines: An Overview ........ 35# \$ {8 ^9 ^: J+ f
Yvonne A. Reid
" \) l- L2 L8 E# K( E6 ~& f8 Z/ K 5 Online Verification of Human Cell Line Identity by STR DNA Typing ......... 45- O/ g! F: N) _4 W @" k
Wilhelm G. Dirks and Hans G. Drexler
8 c- _" p8 k; E* k 6 Cytogenetic Analysis of Cancer Cell Lines............................... 57 j3 T/ B5 z- J) r2 Q( N3 Y* a
Roderick A.F. MacLeod, Maren Kaufmann, and Hans G. Drexler 4 E% T* _ k! K. B& L* p* E/ X
7 Cell Culture Contamination ......................................... 79
9 W) n5 u; t6 m3 @3 ^) a2 J9 ]7 AGlyn N. Stacey
9 L. F+ @) Z" A; m/ }8 u, T 8 Detecting Mycoplasma Contamination in Cell Cultures , }+ W1 U& N+ f4 R' I
by Polymerase Chain Reaction ....................................... 93
1 O8 _% q; I+ _! e- N4 m- l7 S, l* H0 O6 jCord C. Uphoff and Hans G. Drexler / \1 J$ d" K3 I( }- \( s) J* o
9 Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics ........ 1058 I& o6 K1 C6 A- M& ?
Cord C. Uphoff and Hans G. Drexler , |9 S" U+ B5 [; \/ U
10 Quality Assurance and Good Laboratory Practice ......................... 115 u) i) f( I4 B- z- r; t
Louise A. Knight and Ian A. Cree ' E* r9 C% {$ [9 k2 S9 v. [
11 Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning .... 125
* e$ K1 z& I/ K0 ^4 n6 t* bLaura Breen, Joanne Keenan, and Martin Clynes + W+ C- F2 _7 j2 \
12 Isolation and Culture of Colon Cancer Cells and Cell Lines ................. 135
. m% r1 N" g- JSharon Glaysher and Ian A. Cree ( T; r( c! a$ y- J! o9 l+ Z1 d* P
13 Isolation and Culture of Melanoma and Naevus Cells and Cell Lines........... 141" `! W6 d M. J
Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett 2 F9 ]* \: R7 Z9 ?
14 Isolation and Culture of Squamous Cell Carcinoma Lines ................... 151
f" i# _) F% H& f0 v& F8 A6 tKarin J. Purdie, Celine Pourreyron, and Andrew P. South + w2 B! h- j# V/ Q
15 Isolation and Culture of Ovarian Cancer Cells and Cell Lines ................ 161
2 v8 P( J2 s2 x* [: {% J) EChristian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, 6 v- {: O6 _2 h( n T6 l# C# N n; D( p
Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz
" s1 g1 X# z3 s7 j16 Establishment and Culture of Leukemia–Lymphoma Cell Lines .............. 181) ^- I0 a8 `# h% _5 n
Hans G. Drexler
5 q- t+ Z: ?5 a& N9 Z5 u17 Isolation of Inflammatory Cells from Human Tumours..................... 2018 {" Z: s" K) O
Marta E. Polak 9 ^# d5 Q% ]) C M5 d3 z& \
18 Isolation of Endothelial Cells from Human Tumors ....................... 209
3 b. c( e& z. f: D4 V% }# TElisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau,
0 o; I2 u3 z! l1 W# h0 q- j6 URoland S. Croner, and Michael Stürzl / j. N: }, {$ v" z3 Y. v
19 Cellular Chemosensitivity Assays: An Overview ........................... 2191 f+ d- i! R/ u0 Q
Venil N. Sumantran
4 o4 B6 {! q/ r$ l6 r' J$ f; G. l20 Cell Sensitivity Assays: The MTT Assay ................................. 237
4 p/ T- V; ^% X' RJohan van Meerloo, Gertjan J.L. Kaspers, and Jacqueline Cloos
N6 I# ~4 B J21 Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay ......... 247, L/ F+ ` R+ I x. M$ d$ E
Sharon Glaysher and Ian A. Cree # t% p; E8 P- b! Q# P) G/ g: Z
22 Differential Staining Cytotoxicity Assay: A Review ........................ 2594 M/ ?, x; G6 Z' z1 T' _. `
Larry M. Weisenthal
7 u' _: |0 F& I9 Q- Y) O4 h p' Y3 H7 ?23 Real-Time Cytotoxicity Assays ....................................... 285; f2 ~9 A- M0 J: n5 ?& H( y. ?
Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, 0 E$ [' g1 x2 \- M. L4 a" n
and Jonathan M. Cooper ) H+ I4 q+ \/ N8 x
24 Purification of Annexin V and Its Use in the Detection of Apoptotic Cells ...... 293
/ g0 R. c" ?$ Q5 [' e XKaty M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss
1 j0 g6 t& {7 X8 E6 c* ^25 Measurement of DNA Damage in Individual Cells Using the Single Cell Gel $ b6 v3 {3 o' [4 z0 K6 H
Electrophoresis (Comet) Assay ....................................... 309$ R/ W0 C. _; Z! b- f" p# K0 D9 d
Janet M. Hartley, Victoria J. Spanswick, and John A. Hartley 0 C+ g( _. p( M! R4 M1 F
26 Molecular Breakpoint Analysis of Chromosome Translocations
- A1 o! W! O, P, C) k. E6 fin Cancer Cell Lines by Long Distance Inverse-PCR ....................... 321
, p6 K, I& w8 b T+ I! JBjörn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod 6 c# z# `0 x; }% B6 w. q
27 Cell Migration and Invasion Assays .................................... 3339 q" k% t+ L- r# Y- l& x9 U
Karwan A. Moutasim, Maria L. Nystrom, and Gareth J. Thomas
0 S0 o [% ]) ?1 @28 Angiogenesis Assays ............................................... 345
F: V9 S" b% B$ i4 g* BV. Poulaki
" I, {- d( t& I29 Flow Cytometric DNA Analysis of Human Cancers and Cell Lines ............ 359/ T7 E& o" p8 y' e, s6 t# F i8 O
Sarah A. Krueger and George D. Wilson
# X3 i/ E. i4 Q3 q5 q3 @30 Expression Analysis of Homeobox Genes in Leukemia/
& U6 X1 e7 k5 H% xLymphoma Cell Lines ............................................. 371
n8 q! O8 F# w9 v3 J' dStefan Nagel and Hans G. Drexler ' Y% d z- v8 G3 z$ L: |
31 Measuring Gene Expression from Cell Cultures by Quantitative
# V# Y" {0 y# e0 O i+ C8 \Reverse-Transcriptase Polymerase Chain Reaction......................... 381+ F; h8 S( o, J- R/ Q: N3 d
Sharon Glaysher, Francis G. Gabriel, and Ian A. Cree
+ K% B8 a: o# ^32 Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins .... 395
' y1 A; x" P. P0 A5 j1 D& JSamantha Larkin and Claire Aukim-Hastie
- W0 B/ \+ }1 S. E V+ ?, Y1 }33 Development of Rituximab-Resistant B-NHL Clones: An In Vitro
+ U9 w, p8 V& A$ DModel for Studying Tumor Resistance to Monoclonal Antibody-Mediated
1 y* ]; B% J4 S. hImmunotherapy .................................................. 407
" D1 U9 ~. z2 K- C0 O8 r+ d6 vAli R. Jazirehi and Benjamin Bonavida
' [+ n1 G2 K A; A4 \) h34 Analysis of Drug Interactions ........................................ 421
% h5 o! v, e# C2 R0 R# KIrene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters 5 E$ c+ Q8 c1 ]5 F. z9 i
35 Transfection and DNA-Mediated Gene Transfer .......................... 435
C& O; E' d% b% x9 `5 Q- WDavide Zecchin and Federica Di Nicolantonio
6 M) ?! H* ?3 T: A' e, u# KixContents
$ A/ A6 }. a. B36 Drug Design and Testing: Profiling of Antiproliferative ( ?8 w, s6 I* N* X8 n$ J
Agents for Cancer Therapy Using a Cell-Based Methyl-[3H]- / ^) H0 n; m6 ^6 q V! p) l$ ]/ y" V
Thymidine Incorporation Assay ...................................... 451
# Q9 a1 V* ^& O6 o! `) lMatthew Griffiths and Hardy Sundaram
7 R8 z' R- `1 O: s8 T% C5 u37 Feeder Layers: Co-culture with Nonneoplastic Cells ....................... 467+ j* ?! F+ S2 ~$ U
Celine Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South
, x% k# p; a" F& O# U38 Xenotransplantation of Breast Cancers ................................. 471$ E! ]) y. @/ r0 I
Massimiliano Cariati, Rebecca Marlow, and Gabriela Dontu
* g) b5 I4 b1 r8 U$ u, nAppendix A ......................................................... 483
) b- B6 @. S7 F2 O) k, CAppendix B ......................................................... 487) e4 \; }" X% A9 a& N+ I6 C
Index .............................................................. 493 |
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发表于 2015-8-7 19:42
