|
 
- 积分
- 368
- 威望
- 368
- 包包
- 2103
|
《Bovine CD49 positive-cell subpopulation remarkably increases in
3 X4 W, s. \% u6 [mammary epithelial cells that retain a stem-like phenotype》---Research in Veterinary Science
: T. ^. c9 S! ~2 y6 r; P# s- pWe previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential
2 O) E2 i$ c1 [8 x( uto generate a functional mammary outgrowth. The aim of this study was to investigate on the; d& f( R: T/ x2 O3 o
immunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry* A* u7 B( N. L1 [7 o' q
analysis showed different subpopulations of mammary epithelial cells emerging according to the timing of# }& c, Q$ f0 s3 |2 \' z
cell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed,
+ I$ K: ~9 D Y7 M/ M, Aeven if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed
d9 u5 H/ e7 a' I% d8 gfor CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative
" j- M2 U8 n. Z6 v( i8 dcapabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when% |1 J# N _) r' K5 H; _' g
transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation
9 x1 v, K( T+ l6 H$ J6 dthat retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion
, d- s! w; ^+ m* [' p# Jof the primitive population of adult mammary stem cells. |
|
发表于 2015-9-6 16:09
你也太懒了吧！还是自己翻译吧，有翻译不通顺的句子在向别人请教~