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- 积分
- 368
- 威望
- 368
- 包包
- 2103
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《Bovine CD49 positive-cell subpopulation remarkably increases in
; \& }" I# O* N1 O {8 F1 omammary epithelial cells that retain a stem-like phenotype》---Research in Veterinary Science
( F2 u8 v. j0 `5 q. t$ b* j+ K4 \We previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential: U) K6 D: v$ O4 B* q; w8 z0 ?
to generate a functional mammary outgrowth. The aim of this study was to investigate on the3 N: a( P- E/ }* a1 m" v6 g4 B
immunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry+ P- e; N) q. `2 E" Z% C
analysis showed different subpopulations of mammary epithelial cells emerging according to the timing of
: V$ [% B w$ r" q8 Y4 gcell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed,
; x$ j& R% H4 C/ C5 @/ deven if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed
$ A7 a5 P$ w+ Y& t8 i0 Jfor CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative4 |. \8 b6 ^3 Q6 ?% \+ O
capabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when* ?! r8 a9 `" K# z" Y/ x, ]
transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation
! V0 P( X3 l; ?that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion9 Z& j" t' m6 g% o
of the primitive population of adult mammary stem cells. |
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发表于 2015-9-6 16:09
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