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- 积分
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《Bovine CD49 positive-cell subpopulation remarkably increases in
8 v' A T% z5 k7 \$ Hmammary epithelial cells that retain a stem-like phenotype》---Research in Veterinary Science% D+ A& H* v, L# A( d
We previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential- l# i. F! w( m2 p) J8 P w
to generate a functional mammary outgrowth. The aim of this study was to investigate on the
; Z0 ^# v' ^+ Bimmunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry
5 B9 C4 @& Q h r nanalysis showed different subpopulations of mammary epithelial cells emerging according to the timing of
, M+ [( w* l# u: W" Acell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed,
; q$ e/ e! r; F# m' V' p! Aeven if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed
# Y' @/ x) j- G* b' r6 lfor CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative
& k9 y6 f9 G) f% ecapabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when' H; A. V6 ?8 E2 r5 h; T q5 i& w4 Z
transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation0 t6 _" ~1 R7 \7 Y% H
that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion
* S& ^' ?! Q- W. {of the primitive population of adult mammary stem cells. |
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