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《Bovine CD49 positive-cell subpopulation remarkably increases in2 ]3 [! w9 s( M( l1 L4 {- F4 v Z" U
mammary epithelial cells that retain a stem-like phenotype》---Research in Veterinary Science D: U4 a z5 i, E$ j
We previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential
5 w1 F# a" W$ r- Hto generate a functional mammary outgrowth. The aim of this study was to investigate on the
* Z+ K' I% P2 H# Y& j% Zimmunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry
; V- `( D4 h m; W" }% b8 ^! Nanalysis showed different subpopulations of mammary epithelial cells emerging according to the timing of$ z' a/ f& u4 L5 p8 ], u! y
cell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed,
/ L$ ^3 X; p) P. m Oeven if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed
( F& o6 N" R4 R' S/ F& s. b pfor CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative
4 w- r" i% U; J( W) Q! i. z5 mcapabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when* i; ^) X) M* U- g* y% J
transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation0 v W9 u! C* w' R; ?2 T
that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion
' D1 y( s4 Q) F& q) ?of the primitive population of adult mammary stem cells. |
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发表于 2015-9-6 16:09
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