PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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* W5 W3 _4 E/ P" P  f) UCurrent Protocols in Stem Cell Biology 2007年完整版 1451页0 e6 K( b: ?, I# I# T7 U
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Online ISBN:9780470151808
- y) M2 H4 Q$ B& TDOI: 10.1002/97804701518080 n3 ^7 P0 G3 `0 z: G- z0 L
Table of Contents# P9 W9 g+ h$ E- Q0 w
1. Foreword
. C! p; D, j7 v" P- \  M8 c2. Preface
( z# B) Q. h$ A: ^/ n: F# b3. Chapter 1 Embryonic and Extraembryonic Stem Cells8 x' A' G" Z' w* P% N; U, E# y
1. Section A Isolation of Embryonic Stem Cells
4 n2 a( N- n4 G8 \1. Introduction
9 q" \: U- v: j, w' a" T/ p0 e+ J2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells( ^* d2 L6 p7 |! W. |0 U
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
1 i' J6 h  e& x' K, w4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
3 f# e& m$ _6 `4 [; `5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment# A. P. F5 ~2 B5 S2 r8 @$ \8 {
2. Section B Characterization of Embryonic Stem Cells
4 G1 H" f$ @  q8 C, L5 f& ]6 F1 P1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells) }! O; [! e, r" y' i- T: A4 X
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and8 W. w1 d& V& _% T  G
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
( F/ }0 Z6 R: _% [5 F4 z# w3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells" x" C) Z% Q' w. V' c
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of! v+ ~& Y! [" A5 m9 h) ^, q. T, \
Pluripotency' g1 C3 O* p# o
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells8 q; v; n, G& R9 k1 Y- ^3 c& d3 j1 P
Using In Vivo Biotinylation
" N$ k, t# k9 n4 d% h6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem
/ b+ I; m  W! H" g# ^Cells5 ?5 H5 f. F* n: a0 e, g/ p: k! t
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional% V' I; H1 D5 }; t4 z
Profiling6 q( Y3 q" ^, O) _$ q: w& `4 |
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
/ x& T4 ~8 D3 N3 {$ M1. Introduction+ ^% l% ^6 x0 [' g# w: x8 o
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro" e. j4 N* R2 O! \
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture$ ^; C, c# @+ E! B6 E
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
! q$ t7 C) t/ e3 V% N( LMouse Embryonic Feeder Layer Cells* y2 u% d  v0 J
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
' G( r# B" L+ G6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic, H6 I) o) A( I3 T' O
Stem Cells% Q4 a! u0 c: w% c
7. Unit 1C.6 Isolation of Human Placental Fibroblasts% c6 t3 _' o* D5 J( t" [% L
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
. V6 e8 U& W4 A/ z1 nStem Cells7 o. q1 n8 S6 @2 ~; F2 f  i
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of% d0 H% m, ]3 L: P" F* M! ?: L
ROCK Inhibitor
8 s8 k0 [% Z6 r/ Q& @' [' R10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells2 O3 l; P6 M3 W! l
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent+ e5 a2 P3 f9 C" O. |; {% O2 Z0 C$ G
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells7 U7 D0 d0 t5 o& G( P; T& a# L( n
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
9 Z7 @1 ]$ _0 I4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells. }$ u; X9 T. {5 I: v7 T# d; Y
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
0 l/ r$ B$ g0 I( O* l( B/ R1 g2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by( H9 _% l; j. o' B$ N
Suspension and Hanging Drop Cultures  M, W! X8 E% e' h1 u
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
+ `# x  e) r0 j/ }: m3 n7 Band a Description of the Hematopoietic Blast Colony Forming Assay
, U1 r9 S4 l: Z3 I- ~# I4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically
0 m8 O; c( w/ s8 z1 X8 mDefined Culture Conditions$ h! V# H1 D3 b
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
7 U( u6 {: ~3 P' q3 H, B/ e7 s5. Section E Extraembryonic Lineages
: k# u- ~' Z! M2 R" F1. Introduction
) S4 M/ |! T3 Q' f2 K# e5 f2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation0 h, [3 e7 B$ U* r6 F% o
into Hepatocyte-Like Cells: s7 d' `. Y* d/ u
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta# _5 V* N" K9 T1 g# q- `) D3 q: t  ~
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
- u. Z& H# p3 I- B5 Z+ w  A5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells( q% t* W- f; L3 |# W7 E, M# h
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
2 ^3 u1 J5 Z; L! U% _+ T; I7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
! j# f6 ~  s5 S* a" {6. Section F Mesodermal Lineages) Q3 H3 q& r: y+ S9 Q3 q
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
- p9 K0 s% x4 O9 }0 k. ]3 r2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture4 W  e# F; [5 X0 U1 D9 @& ?3 k
with Endoderm in Serum-Free Medium
" F+ @7 @$ k: c1 w2 U- P3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells# }3 K: Q% A8 V& ]& V  o
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
6 c8 Q6 ?! E8 S$ g. D& ]  G. \5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
3 k' s7 [& u& o9 H6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation+ S; E  J/ U2 t% `  `, X% n
with Stromal Layers5 I' v7 ^  h; N8 i! O: Q( F
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
; ]% }: D! @+ g. ~# X$ S: L3 p5 D. S* N7 qMurine Hematopoietic Progenitors
6 r& @% @/ |3 b, d- l8 k. y8 k8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
! T9 J$ g8 L8 X! I, OHuman Embryonic Stem Cells/ Z% g' \0 i4 b3 V# r3 p1 W# ?
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
" P# K+ u5 c0 G- I$ ?" I10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
  V2 H- E: z9 j* F% e5 V+ zProgenitor Cells+ O8 D4 s  _, B4 \  p* A7 b
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
; U% l2 `& f; iHanging-Drop and Mass Culture Methods
' B2 W! l# d9 z5 W$ }7. Section G Endodermal Lineages
0 ^' ^, A; A4 S: j- d1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
0 H1 w: Q8 m% v! T9 j2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
0 `0 R  H9 k! l& q. [3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm, Y2 t+ w) I" p& o  H
8. Section H Ectodermal Lineages
% M+ X# ~9 L! _8 _9 p6 j1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
0 ?+ f2 w7 _6 k2 ?5 h* d2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by6 M1 P6 _/ V+ u$ Y3 @
Two-Photon Microscopy- g5 U( @2 _; }2 p3 N% T4 @
4. Chapter 2 Somatic Stem Cells8 x2 B; [! j8 V' A: K
1. Section A Hematopoietic Stem Cells
9 ?! O2 E. P: ?1. Introduction+ w0 \8 F: X  [- u
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density2 R1 l& g  j. s
Gradient
/ A. @1 H. H4 e% l0 z- H3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
* S: `6 A/ d& G8 ?5 e( t; P4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood" T- o+ W9 i- a+ H  d( i9 O8 Y+ q0 o
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
& }8 w+ d0 A- `! E5 P" Y* s$ Pfrom Adult Mouse Bone Marrow
2 r! d2 d4 @# W) M6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
; o- L! H# m' K$ F) a% x7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine6 x% [9 }" `5 v
Embryonic Tissues
" F  C+ P: r3 B7 C% s8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
; W* Q3 q8 s  [# N6 n/ S. f/ u  K9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
* \. y, e) I( n) w+ W5 l* W6 P- s10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
: d) D  r5 ^3 J; f6 c2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
( f' I4 S2 i- p% O1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human6 \7 I- s! n! `1 ]( R8 {7 T
Tissues
  k' @/ n& Y) K$ r" P3 g6 u2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells3 g+ x0 ], o. `
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
# F( E0 w$ `: P2 f3. Section C Cardiovascular Stem Cells* W& e# l3 K4 ~. `% u" c- k5 T
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
( H0 `5 D  f0 c$ c2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
! [, C+ @5 d$ ^) h3 r3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells& ^" s: }9 G9 P0 }: T2 Y
4. Section D Neural Stem Cells
) y9 Q& U. d- l$ v, o8 H; U1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
) L* `1 e3 W' d# Y2 m3 }2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells" f; `/ m& v! d: A9 i6 [
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and5 ^5 Z8 L. x3 d  A+ E, v' _$ {
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
, Q7 |3 b2 d; J& `4 uForebrain Neural Stem Cells7 z6 F; c& G* W: x5 Q4 P
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage3 Y+ A8 W' O2 V  z1 K- d
Progression, and Maturation- D0 K( Z; }1 ^. Z
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic& |% G7 ]# q- z2 t/ _- x
Neurons from Rodent Brains
# V; }+ r/ e9 C5 S/ U: P8 X1 w6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere% g9 G+ d" e8 B5 G; Z, _
Technique3 }* `5 m3 R4 G  x5 c
5. Section E Germline Stem Cells
4 K! b+ C/ ^# \/ m2 e( x9 f6 S1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila% M  T! }, E9 c5 B0 Y2 \
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
2 F9 y2 O7 Q- C* L) f0 ?6. Section F Gut Stem Cells
( k! D$ o$ u6 o1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
6 O4 l7 X" a% o& U7. Section G Lung Stem Cells& R3 R0 w# S& z8 C1 w
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
* l; I2 r+ T4 r& G% E% \5. Chapter 3 Cancer Stem Cells
2 l4 `% M& y* E; L. [: Y7 `1. Unit 3.1 Colon Cancer Stem Cells
: d3 W8 \& L4 A6 O/ a- J2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
# O/ Y: w, H) ~% \8 Y3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
" Z- a4 G' o: s) I6. Chapter 4 Manipulation of Potency) P9 D3 m  L$ z5 c2 f: d
1. Section A iPS Cells- |! {. Z3 W/ y
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
) l" g! X1 {, F& _+ f# G& T& z2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
4 |; k$ z3 ?$ t6 m# g1 R2. Section B Nuclear Transfer3 Z5 F* t% S2 l! G; W2 Q
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency- _4 t8 K: |3 H) ~6 }+ ]* Y/ X8 G
7. Chapter 5 Genetic Manipulation of Stem Cells
; ]3 E+ {# B5 E! e1. Section A Lineage Tracers in Stem Cells
4 E( U: b" o: T5 N- \: c0 N" s1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
- r& o" n& i* p% s7 ~9 W2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
+ U; J3 @0 }, H6 b" {/ V; B3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
9 ~& }. M' |& l7 ?% p4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium4 Z9 p+ A" W' ~% P4 l. }) d  ~$ f
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
8 s# N; U- b3 M( m4 k5 @' qNeocortical Neural Progenitors
/ f0 s% @9 }. r2. Section B Homologous Recombination in Stem Cells4 d/ ]  X* i/ p
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
! R- s, M; s8 B* rHomologous Recombination  N2 @+ Z, q, w& x# n% v4 ?# _; h
8. Appendix 1 Useful Information3 q6 ~" F8 }( b$ B9 ?" [! C
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research" P7 D  M( r! Q; T; O+ D7 c) K& L
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells! B- m' d# v( P
9. Appendix 2 Laboratory Equipment3 w# r' j& ]( ^& r' O2 T
Standard Laboratory Equipment2 Q* o% w: `+ I) Q  ^$ }
10. Appendix Suppliers
* H' s' O  g8 ]8 L0 \$ gSelected Suppliers of Reagents and Equipment* U; T3 V/ N2 I4 |; a

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