PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页( K7 G/ U- V6 L# v/ @
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Online ISBN:9780470151808$ Z% m: f6 Z; D! c' D8 X
DOI: 10.1002/9780470151808' ~- P) ?) g6 s& h& w& m  i+ v
Table of Contents, X. n/ T- D, t- ]: R  I2 K* y, ?! t: \
1. Foreword
# `% P; E+ f. S2. Preface
0 p! w' q% c) {4 M0 y3. Chapter 1 Embryonic and Extraembryonic Stem Cells
6 G4 d0 f1 q9 @7 r# E1. Section A Isolation of Embryonic Stem Cells
0 v. U' b  n: C9 g# F1. Introduction$ g( v2 g) {8 v) [
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells9 r! h" S9 P; S5 |0 T
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
3 ?$ e2 B9 M( B. |3 R4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
4 L1 R2 C, X, H5 |, Y& x6 v, M1 c5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
! m/ E1 D2 O. n3 k+ ?. u2. Section B Characterization of Embryonic Stem Cells  ~% x8 Z& S$ H4 J- o7 v
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells% E' d, G; n7 I5 C
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
8 G' N( P5 u) c( kEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform
6 k6 m0 J2 U9 j0 W- x3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
, |1 U' a: F. F$ _6 l4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of$ H$ s0 ?' L# T; J
Pluripotency. k7 V* C( A7 F; a  t8 C: J; f
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
( q) c+ R5 |; t3 u7 _& ]Using In Vivo Biotinylation# S+ `( [5 |" k
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem3 J8 Q) h2 v0 `" y4 E
Cells
, f  |, ?8 L% c. d: l. `7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
2 X, e% c  d* u8 }1 W; mProfiling6 P* G5 {3 o2 _! Y+ F
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells$ ^9 o0 u9 W/ G' |" K0 A; u
1. Introduction
* b$ \( g# S# S) B; V- N2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
% }+ f& |9 d( V3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
; x: _9 r" t0 a/ {# k4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of# o) u. O3 X$ U. r* Z
Mouse Embryonic Feeder Layer Cells* K: i1 f6 g7 }. o  c3 k' H% v+ E
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
6 C  l1 x7 T9 H* d. ?$ a) H6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic2 W9 a9 U. i, W: r
Stem Cells: y  d) A  U9 ~) a& T. C
7. Unit 1C.6 Isolation of Human Placental Fibroblasts  ?* b5 `. j8 d& x
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
6 q2 [8 ?9 A  X( P7 [Stem Cells
' ?3 C; b) K" h: `9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of
0 D2 j" D$ w; ^( q1 [ROCK Inhibitor
( O2 h* ]) _" F4 U3 J10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
$ y  F" e. C! v! v11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent  N) I* k! C; w# S8 c) Z2 ]
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells( N% G* y2 N# n  Y
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers& k8 B* z6 H$ Y" ^2 y- a2 `
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
8 V( B0 t/ {! w2 S0 }: W- n; k$ ~1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
; P: N- D: E2 K) S7 S! T& z6 }0 [1 p2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
3 J& t( a: s9 o. H- lSuspension and Hanging Drop Cultures
  _7 {8 a5 W0 |& x6 c, M! B0 S3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
# i! l; [" R+ Cand a Description of the Hematopoietic Blast Colony Forming Assay
! t3 f7 d( W0 j/ b, r* k4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically
% c3 o) Q$ S1 W8 t; dDefined Culture Conditions0 S- w+ p! S6 X+ {6 ~- g: N" j; q
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
/ j. p" M3 f( W3 P* ^) H5. Section E Extraembryonic Lineages- i8 w0 Z  S+ T1 s; T) R! S; ~
1. Introduction; u( u" x3 Z1 e" G* v+ i
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation" K. e4 i8 \) A# l3 I1 Z: s0 z
into Hepatocyte-Like Cells
3 @. L9 A% {: r2 H" W3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta$ N4 e  S% o% `4 y# m4 r  f8 L
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
7 I9 i6 L  {+ @& f5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells5 Q3 _, J2 k0 i# `0 M5 }0 W# M
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
$ c$ c6 v/ {# Z: o8 v4 ?7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
, z. P* a0 c: I$ K1 b6. Section F Mesodermal Lineages
: y8 ~6 k. s, L, G  v, ^1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
/ x& ]6 L+ f9 H: E& i( r% S0 o' W- r2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture6 R) y+ d3 s0 B. W! q
with Endoderm in Serum-Free Medium
( e/ q) T& O; K- l3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells3 B2 U2 o" A6 z+ Q, l; U1 v) l
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
# ^1 N  U! ]9 b$ O5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells! m# M* `$ n/ V. Z& m
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation1 b7 x! ~$ d3 G5 @0 K; c
with Stromal Layers
+ n  M) _+ i% y) {' B7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary- a) C/ T4 i/ q0 n5 X
Murine Hematopoietic Progenitors8 k$ N9 R( h* i2 z  v% D2 C
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
* g1 B: W/ R# f7 @( _1 U# A0 C0 rHuman Embryonic Stem Cells
$ Q5 ^" S8 A- K3 ]9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells7 b$ h5 p; ?* p& u9 D' h
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac1 Q% o" Y- G( ?/ @
Progenitor Cells
- E, J5 E' o  s11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the5 V3 U' y' R& R: w
Hanging-Drop and Mass Culture Methods
2 p- x! X% _& I7 @9 O* |* f$ ]7. Section G Endodermal Lineages
+ V3 N" |4 R2 P+ t- ~1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
% S! H. Q9 {4 _; \3 d+ t, j# y2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
/ n' K3 t4 x2 H; v. y3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm( m3 v% W) N5 {( n  I
8. Section H Ectodermal Lineages7 t! A; b- _5 ?, O- x  ^
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
& K3 ^/ q5 h) @3 D0 c% N2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by9 i, e3 C; O* t$ u
Two-Photon Microscopy
1 W+ T; S- E' {) G  S4. Chapter 2 Somatic Stem Cells* n! z* L/ x+ O. w  `
1. Section A Hematopoietic Stem Cells& c" C5 t6 Z  H9 ~% O, T& A  E( `, z8 K
1. Introduction
. Q$ V3 o8 N, \2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
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3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood. l9 d( i5 `1 T' j9 ^& |
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
" O9 W) ^! ]1 Q. h3 {/ D5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells& w; ~$ Z1 ]5 l7 b
from Adult Mouse Bone Marrow
9 J+ g0 H1 z/ a* S9 |/ ]6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche) b$ O; j; q& i
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
" @6 ^2 z4 C: i9 _6 }1 rEmbryonic Tissues+ z6 e7 W4 a9 T% a7 m. [
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells. Z/ i: v$ p) o4 i. R
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells9 ]- I* E: J! u6 D
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
2 v, U& a; J2 E; B4 [& i5 h2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells4 G. z- A, b; M; w7 n2 Q
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
, V8 V& Z5 v5 U' }; CTissues
% i+ N2 B. u9 H4 T7 S% s, x* R5 N2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
, L) \( ?6 ]. `0 a/ t3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells2 U8 ]& O: H8 e' c5 \
3. Section C Cardiovascular Stem Cells
2 x- a6 e( I& l' ?" u1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
7 v( b' X, w# K5 p# o2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
( r7 i7 S% m: W# L1 P3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
5 i; }2 Z9 ?" J1 I4. Section D Neural Stem Cells1 E; J: _- W4 F# q* [7 M2 d- W/ t
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
. N  j# Q2 ~1 n- n, V2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells' X. ~/ ]; s+ ?- B
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
& [0 l+ {( f' ^7 K9 v; _0 BCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
" e  |# N7 F, k& C8 sForebrain Neural Stem Cells( o5 j: f: W3 B) D0 h  Z6 P
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage
9 ^- D7 a0 z3 o9 G5 P4 M1 tProgression, and Maturation
. p6 C8 E# {, f; ~3 N, l5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
" I9 g% d- ~# s) u, d2 YNeurons from Rodent Brains
$ q! |& I) J4 O/ f6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
9 Z1 C5 g! S5 y0 P2 g& m  OTechnique
$ b) }5 t: S5 j, z. s5. Section E Germline Stem Cells+ s! I- }9 |+ V
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila9 r- l1 K& W( m& i5 i. h9 |
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
! h- B( `: _# A& ~3 j( x6. Section F Gut Stem Cells+ A  X+ d/ Q7 K; U
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
! N. k$ }5 U1 n+ b$ P- n! {# G7. Section G Lung Stem Cells7 U4 W7 z- \5 G& c. G, V
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells" c9 ~% |& y3 m8 }5 `% ^! o
5. Chapter 3 Cancer Stem Cells' V: a5 C& Z1 n
1. Unit 3.1 Colon Cancer Stem Cells) a( Q/ c1 I) i1 w
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
# w/ A+ M9 p6 n/ s$ _. }$ x+ a3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells2 N% t0 ^! t! \( h/ L+ }7 m# D
6. Chapter 4 Manipulation of Potency, f% d7 X0 z6 T- J/ y
1. Section A iPS Cells
$ t$ Z' Z8 {2 S) Y% [& g1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
4 s! S+ @- Z/ h/ A/ H' S8 X2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
4 w+ N( L( G* {" U: S  D2. Section B Nuclear Transfer
% j; a/ V2 a! U9 }* U6 s1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency+ [0 w0 ~: k( L% `4 f0 n! x0 N
7. Chapter 5 Genetic Manipulation of Stem Cells
6 j8 D3 k! P) X* ?# v  K. K- b- p1. Section A Lineage Tracers in Stem Cells
: k! ?' P& N, m4 K9 |& @1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma; j" |1 C. Z) b& X! h
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
% C* t; {, V9 ?2 J5 k6 f3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells' V/ R, N; R- p; p! t  T
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
  B* F) O8 _$ X6 {; V3 M* R4 L3 M0 s5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
3 g& o. p) R0 A8 R, G. uNeocortical Neural Progenitors
3 x# }7 f+ g$ n% \' U# ^2. Section B Homologous Recombination in Stem Cells
3 z3 _/ V: p# q1 `2 [6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
- b( n& w% y. F2 P/ v6 n% W1 dHomologous Recombination
/ a7 a' w2 _# x+ |; `! N& d; u8. Appendix 1 Useful Information/ }3 e, z) s; `7 c( [8 x
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
: i7 {0 J* W7 X& L+ ?, V2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells6 L* ]: i& W' s( F; [
9. Appendix 2 Laboratory Equipment$ g6 {1 d; @+ S9 J+ ?
Standard Laboratory Equipment
' T; }" y2 t8 T( ^10. Appendix Suppliers1 Z' o1 |# i6 _/ U6 M0 J& P' X5 G6 W
Selected Suppliers of Reagents and Equipment  f2 s9 V5 o- d$ _/ p

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