PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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7 r5 c; H! |) |Current Protocols in Stem Cell Biology 2007年完整版 1451页
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2 [* I, o, S' Q- I" x, wOnline ISBN:9780470151808/ R$ _% K# I; d
DOI: 10.1002/97804701518083 b) {9 ]- L2 }
Table of Contents
3 N! S+ x" ?: y) l& b1. Foreword% F9 [( Z& d2 Y
2. Preface6 E* V. h0 Z2 e$ K8 Z
3. Chapter 1 Embryonic and Extraembryonic Stem Cells
, r* y3 m; w' Y# D: m8 Z1. Section A Isolation of Embryonic Stem Cells
& L4 l; w  q5 Z, t5 k. N1. Introduction: u- M" T: J0 C; C- _) M5 D
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
  h! c/ J& O: {4 n: N/ R3. Unit 1A.2 Derivation of hESC from Intact Blastocysts+ h1 J  W* S4 k( V- M
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells& ^. W- C" A+ Z! N- v
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment, j4 B/ {% v! i( a$ E# ?8 w
2. Section B Characterization of Embryonic Stem Cells
, y6 ?% E" R6 }3 {* c1 l1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells+ _; B* _8 C7 u( u
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and( N9 _. {$ P: ~; g8 p
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform0 M5 M' U7 G; a1 s0 H% O& B1 Y
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
9 B; s% Y6 ]- O4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of( J& w/ F5 Z* h, q
Pluripotency8 {2 V, f3 f) T4 N- z* {* k
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells! f& w6 e& F+ V; p. t4 T  N$ d
Using In Vivo Biotinylation
4 S9 L& W* c% R# a3 I6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem$ I- e' C) g4 V+ o1 c
Cells
3 P& Y1 V. g2 d9 ?# }. v7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional  |9 F; ^4 u! K8 Q7 p, b
Profiling
, c% n0 A. g3 v9 `3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
' Y# @! {' x  k1. Introduction7 e7 ~& ?% ]  i1 j3 m. P) v$ y, C
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
& u3 Z/ ^5 u1 B6 [3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture* i* E- ?: m: y2 g! c9 K
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of$ D0 H: ^0 @* L3 z
Mouse Embryonic Feeder Layer Cells( S! L3 g( w6 X6 s( V/ c# F! P1 r
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells+ ]0 V0 E* @% `- ~( h  \. F3 R
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic% c- Z4 b- l% Y, T3 j5 Q# z
Stem Cells
3 n" _4 O- ^7 ]& L7. Unit 1C.6 Isolation of Human Placental Fibroblasts
; R* o6 F/ l: `8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
; \% D# |4 @( u3 A8 V3 o* pStem Cells! Q) h: K& Y  O. y; i$ e
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of) H0 y, D$ V" Y8 M$ o
ROCK Inhibitor
9 M/ H" v* k9 a/ o10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
) x6 W4 }9 y" K7 B9 u  {2 t11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent% \# U$ `8 D, k( f
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells/ h! S. N0 a. `8 B3 j% @8 h5 [
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers$ t* K) ]: E0 B3 `, M( i; Q
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells9 y/ f& Q2 [  l7 k5 u* Y
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
* L$ d5 Y% `5 E5 p8 |& ~8 a2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by; `7 t, b; t/ m1 o
Suspension and Hanging Drop Cultures
/ L! Y% ^- ~3 Y, Q) a+ g/ C3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
2 q) r$ d5 _/ C+ J+ ~5 `and a Description of the Hematopoietic Blast Colony Forming Assay
6 L5 u$ K) _9 t  P+ a- w# }7 B4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically
) I  z! {: L4 t4 HDefined Culture Conditions, K2 ]6 i" O8 V$ t$ }* w
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells! I+ p& e9 k" |$ ~, a% S/ ~$ \5 K
5. Section E Extraembryonic Lineages! \2 a* m# f& o% j; x
1. Introduction
* F5 @8 ?, O1 E: N( g2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
6 L) O# N- a: C; M3 I; t* \into Hepatocyte-Like Cells
1 p; `% |) Y# [2 F7 M3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta: U  h8 ]: J+ u% N
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells$ [- w1 I$ E& }8 L
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
1 [! c  e0 K( e9 l  R6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells) g" j: m0 o; L9 ^6 g) f$ ^
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use& d/ S$ `' [' w8 e
6. Section F Mesodermal Lineages
" g5 \7 [7 R3 i* Y2 K( B% ]2 C( b1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
/ ^" T4 E! e( ^2 s2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture- j, B6 F- o( S, \5 W7 b. E
with Endoderm in Serum-Free Medium
5 @( e0 |( D% T3 d3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells& j, \4 R, o& G
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood* [3 M% G0 `" m  k0 t: U
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
+ D6 e' d. R3 q4 F/ C; e  ?9 l6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation
+ N. |+ h4 s. iwith Stromal Layers' P) h  d" b5 z
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
. X0 }0 L6 o" X. A' h: \" [Murine Hematopoietic Progenitors2 Y3 s( s' q9 H: h+ _
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
2 A- ]# M5 S/ N9 _. m4 L! [- YHuman Embryonic Stem Cells0 a0 Q, v# x6 O" @
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells& r7 {+ ]" L3 m3 N& m7 [- k# f
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac! N6 Q9 Q3 Z6 w6 L+ i
Progenitor Cells
) M8 b6 W4 [$ X% ~8 Y  x, d11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the$ C0 E* D* e$ x" u2 g, e/ X3 g
Hanging-Drop and Mass Culture Methods
& ~! ^7 m  n) B# U+ U0 k7. Section G Endodermal Lineages* X7 b" J* y7 E6 {2 R
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells! K. e/ l. k4 ]1 Z
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells4 y5 S! @; ?3 ^0 g+ A5 I
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm7 j" t7 |' r1 k
8. Section H Ectodermal Lineages
. i4 h5 y6 I- m1 G, e1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons3 A& a$ I0 q  ~* ^# N5 d/ J) c
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by- R! ]9 v7 ]9 ]+ k: B, R1 [& ?$ G
Two-Photon Microscopy0 A3 L( q  s" \1 A0 p
4. Chapter 2 Somatic Stem Cells7 {4 }4 o* `7 d" l- [7 G
1. Section A Hematopoietic Stem Cells
: R3 w2 b3 s1 U8 M1. Introduction
" x/ K% n! T' W2 [8 z8 x1 m2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density: @' {; v" t- e; S$ `0 x% I
Gradient; J1 @  @7 m: s7 R  Z, B
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
% ]% }( B9 h# b  S+ T( N6 G* k4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
& Y* M! ^5 N6 x7 v5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells, K$ k+ h6 [7 l3 l( W
from Adult Mouse Bone Marrow
, u0 `$ U4 A$ ?6 N# ]8 p! T6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
6 s) ~% B; Z  X$ S* m; \0 G" |1 B7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
6 \* h3 m% }) P& h# }% oEmbryonic Tissues
% F. D! \0 f6 ?& X/ X. o1 j1 I8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
/ x* c* }7 y) s9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells" Y8 y+ l4 n3 e9 r( t
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta% Z8 s4 I# Y$ ]( Q1 N* J/ \3 f4 ]5 N
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
# S# M$ K- i# ], Q+ g# y1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
+ y( H$ B. H) r9 J2 a: QTissues
$ n# y3 o8 Y; N* R  v, P. V! u2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells. d" o% t+ M; g5 i! B* t  v+ {
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells, _4 H# B, y% c+ M6 s0 w/ n! f: K  o
3. Section C Cardiovascular Stem Cells
; }) C( D8 u. v8 E- L: V% z9 I1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
) z2 V! b; P: [  O. I2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
' f; h1 M) ]$ n4 O; t& ?! O3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells- v$ W8 D4 D8 r% L* j! G: w: [9 D
4. Section D Neural Stem Cells$ l" t5 }2 k0 ?; G. T. E
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains& J! N. N5 Y. l1 p/ e% f9 ]
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
$ h0 j1 w4 R& F& G* u7 ]6 R+ S3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and7 W7 m+ S  b3 P1 b6 r
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
+ g/ G, Z4 D) M8 D6 X  q" S4 CForebrain Neural Stem Cells
& b0 K) }0 U; h' I/ h, G: q4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage7 |: @+ J+ H0 y& Z  o: `; X
Progression, and Maturation
: n4 u" J; e( V- F5 k2 g5 m* B8 D4 r5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
3 Y4 U! }6 t5 \& T7 KNeurons from Rodent Brains+ D& a! n% k; u4 ~1 i- }/ ^: l
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
; C9 W6 a! X- V! r* A% xTechnique1 G9 Y- ~8 @6 p2 ~
5. Section E Germline Stem Cells& Q. O' \' d3 r2 I. e# \) a
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila# J' E- j, ?0 }$ S& p
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis$ F8 L$ y- i  h  k
6. Section F Gut Stem Cells
& V/ C$ s7 ?' v9 i( ]" J8 I1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells8 f) N1 [. ~1 m) h0 M$ v
7. Section G Lung Stem Cells9 P1 c! V2 \9 k9 n2 I4 b
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
6 h$ ]9 S& g$ }. _! Q5. Chapter 3 Cancer Stem Cells
$ i! a# e) W: [$ ]# v1. Unit 3.1 Colon Cancer Stem Cells  P. b; J- l( C: f8 g
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
! E4 v2 z0 t1 s! f3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells) i" F. a" O; u$ J' j! C) V9 F
6. Chapter 4 Manipulation of Potency
# {- s8 F# [4 B' i1. Section A iPS Cells
3 d6 m/ t8 }) c8 x" W8 \1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
; ^. y. v  A9 c' G2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells6 d! t1 f/ N2 p# ]
2. Section B Nuclear Transfer" L: W  K2 }! `8 ~& W1 D+ s( G
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
- {4 _( D4 F* W. j$ e7. Chapter 5 Genetic Manipulation of Stem Cells
" K$ g( r2 [6 I4 h1 p0 D! g1. Section A Lineage Tracers in Stem Cells1 s" R; h5 F! S) }. F% u
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma1 k$ a7 e. U, t) C
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
: i( o$ f5 Z4 P$ @- F3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells5 T4 h' ^# k4 A4 F3 i
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium; ?/ @% f! D7 r/ T0 a
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
. Z; A9 |! ]% tNeocortical Neural Progenitors0 F" L# P# X6 b. v* o
2. Section B Homologous Recombination in Stem Cells
5 m) F7 w8 ]* d6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
9 P- w2 F1 p8 u1 e8 lHomologous Recombination
  o1 s  I% y$ j' Q0 [8. Appendix 1 Useful Information
2 K+ F' q0 c& p2 f4 Y1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
" i* B# X# X% M9 U" R$ y/ Z2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells6 t: ^4 ?: p; ?0 e) y
9. Appendix 2 Laboratory Equipment
8 @( S1 r4 g; J3 \Standard Laboratory Equipment+ B2 b  ~8 U4 o
10. Appendix Suppliers
( A- m' S: ^. `$ y4 H* dSelected Suppliers of Reagents and Equipment/ |$ w4 v0 r1 U& W( P$ p

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