PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页4 s! r# e- k: i( s

  J+ u$ ^1 k# t' r9 rOnline ISBN:9780470151808
& p+ k8 |0 @3 t. }1 K( c4 ADOI: 10.1002/9780470151808) v+ a  i. Z1 m# s
Table of Contents0 `! s6 w( e/ u
1. Foreword& y7 @- v" s  o7 {
2. Preface
: O9 |( n( d* @* o  o  P  L+ s' n3. Chapter 1 Embryonic and Extraembryonic Stem Cells
$ f5 n* U8 |8 U4 {4 H1. Section A Isolation of Embryonic Stem Cells
3 h! a8 ]: `3 u1. Introduction
/ z- e  B0 X& F5 ]% B/ [6 a% }2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells& P: |# }- B% J5 J4 f; ^2 w! G3 x
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts7 E7 F3 M; T9 j0 _
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells/ n3 _& J2 u6 }  f
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
. Y5 @. a+ \9 Y, A2. Section B Characterization of Embryonic Stem Cells+ T% C8 a7 C( h) Q
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells7 K  @9 y" q+ p
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
- q, T' J+ h5 Q; Y3 aEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform
0 p" t: C  k4 @- @$ D8 q3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
) `- D) |) c. y! A) o4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of5 u' _1 n$ k* W+ S# P* q2 J
Pluripotency
- Z% k' Z8 j$ R( A' [* N5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells, A1 J' |! v6 r7 O, w& K9 U  t' ]
Using In Vivo Biotinylation, F, z* f, D1 t$ i' |8 X
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem
$ I" V. ~' x- E% a/ gCells
' c! {- h7 W7 o- ]7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional4 C8 h- A' s8 z% ]  G4 y
Profiling
* b  ~7 D% R0 |/ A) _8 u, F3 b3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
) C9 ]/ \7 X$ S. f# s' {- n. P1. Introduction
6 k- y5 Y) ^& |0 Q+ p5 t0 j2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
+ K4 g% H: x) @; S' J# R3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture: m( F2 E  k9 J- M: m& @
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of9 |$ g' o# _, ?3 i$ ~) S2 c
Mouse Embryonic Feeder Layer Cells: l1 P, C+ t8 _  `6 C( @' U, S; ?, j5 G
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells3 k2 ^# g; O; r. Q
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic; ^% s' Y# L0 ?9 Y; h, E4 {& Y! |
Stem Cells
: ~% l( |! f0 P7. Unit 1C.6 Isolation of Human Placental Fibroblasts2 h2 @6 a" \  d8 F0 }9 A9 O) x
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
3 `+ M& ^. p# Q4 p4 \, o0 ^Stem Cells% p6 p) T, W6 K/ T; D
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of; ~2 Q: @) u. ^( Y4 F7 x
ROCK Inhibitor% b. P7 L3 @8 O' k' F. p
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells- G4 @; b' s( Z2 N
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent% p6 x2 r2 b. ]: t/ k; V
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells" ?. H% [; q7 Q. e1 ^$ g  R6 G
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers+ h5 B; N! J/ r
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells% Y4 G: B" `; _5 F
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap' b$ k, ~% T! `' \1 Y( ]
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
$ E  T  @! W1 h( R. CSuspension and Hanging Drop Cultures  S+ v! [; d" I; i, q
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies" L$ A1 o5 m  v; ?' {, p: Z
and a Description of the Hematopoietic Blast Colony Forming Assay7 b; W2 f) p4 r  i2 [7 `" j) r
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically+ g' S! I2 z( y5 K7 p* @& G
Defined Culture Conditions
  y3 u# a; G0 |0 D0 F* y+ a: z5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
" `& ]; t( _! c) b% k5. Section E Extraembryonic Lineages( `8 M# g( k4 g9 F" H2 I
1. Introduction( O7 |/ t9 x8 g7 {5 O5 Q7 t
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
3 n8 }# D2 h; d# a! K# }/ \5 J3 pinto Hepatocyte-Like Cells
* X4 G$ s9 Y4 Q3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta0 p- R8 h6 y1 A! l' L9 h
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
. U' L7 |2 ?: \: v0 y5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells3 y3 A! [; a% A1 s- r
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells1 c) b+ k! e0 ?" L' a6 u9 ?
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
6 u" j5 h, J6 [! P6. Section F Mesodermal Lineages
/ b$ m3 x% T& _! _% U1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells6 L9 y+ H7 a, g+ V: t
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture) g% H1 A- G3 n/ j0 I
with Endoderm in Serum-Free Medium
* B- X6 i9 B5 V1 R. j$ B3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
) \# g- c' L5 y4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood' T0 r$ s7 d+ L' K. n
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
3 R; h% ?' G+ d+ T! b6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation. P2 l! X1 V  a, G. q  w0 Z" L
with Stromal Layers
( O% J7 s  o( ~8 I" B. k7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary& C# n  V, k6 [1 K/ ]6 W
Murine Hematopoietic Progenitors
4 J$ R. s1 S. V+ N6 L8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from* K8 l6 F5 Q: X3 I& m  T
Human Embryonic Stem Cells
2 a9 m2 D5 h* E* _  S. u9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells( f. }2 z- h* @' h  ]
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac" u' x% P  {% B. ~/ m
Progenitor Cells4 s% B) n9 ]  B7 f: L
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the6 t; Z! n, Y( m
Hanging-Drop and Mass Culture Methods
, P0 H4 t# A5 X! `0 V* I7. Section G Endodermal Lineages. Y1 T; c5 [" f1 i1 |
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
. \- X( m! _/ l8 x2 g5 \! R  \+ \2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells' Y3 J& i1 v+ p0 U, R* V. a6 ?7 r
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm/ |$ i' e3 i. o' L2 L4 G3 P$ H$ C0 l
8. Section H Ectodermal Lineages
0 c2 I0 m1 s* M& e% ~1 Y1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons3 s, w  L5 r" V9 K' T
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by
. o+ {# N3 J- r, j. I* t) @3 y) {8 `Two-Photon Microscopy
( Z7 `; d: U9 C* g4. Chapter 2 Somatic Stem Cells
, E" t, y) z# b1. Section A Hematopoietic Stem Cells/ V4 s! S8 H, A  c
1. Introduction/ x( X+ |, i7 w! Q3 T% o3 n" N$ e
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
, }+ K3 h2 N2 d) I  e$ N% tGradient
5 Q. q( _$ D: J8 c/ R& h3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
/ u+ Y, A+ a% h9 X+ y4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
5 R5 p% {( O8 C6 G+ J# P9 V5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
, O2 l/ E7 B- D3 o8 K0 C* e$ O8 |+ C' vfrom Adult Mouse Bone Marrow$ S( j3 v7 h" h; N) K  |% A# }) Y
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
( o4 U6 h* e! F9 q0 Q  w: x7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine; e) M" ~& L0 z/ ~' z: v: u
Embryonic Tissues
) K% }6 V- N' B. H3 N% _8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells+ B! q8 l0 H  M# N7 h0 }
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
" Z# G, z, X1 I3 S$ B10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
9 G5 c' `, m; ~# w2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells  [" X3 P' V  p7 Q9 }
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human( c% d$ y( R0 _
Tissues, l% X" B$ q, ~, H0 F/ v  ]
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells: V0 r5 |. n# g' }" c$ C7 H
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells% z6 t; ?9 |$ y4 D  T" |9 L
3. Section C Cardiovascular Stem Cells9 M9 n/ H7 g2 Z, w' ^0 M. N
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
: f- Z& z' E4 K( U+ Q" _2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium: x3 c2 i" ?6 ~) X1 y  ]( B' h
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells/ ]! u& l; o% s, S
4. Section D Neural Stem Cells
3 B$ s) n/ C! P6 T3 ~2 H6 d9 Z1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains, N2 J5 D# n1 `. I% `- @9 R; l, I
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells8 ^9 L6 ~/ e3 n% A9 J& H4 f
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
3 e8 h6 y( F" p* i- N6 G9 NCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal$ i  N9 o+ C( E* }8 O& i9 w( p
Forebrain Neural Stem Cells
4 r5 ]: n/ `! f' {4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage. |8 Y, E" T( C  i% h0 y
Progression, and Maturation
/ E# Z6 G8 e5 T% j2 Q5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
% ?  P+ O: q$ e) g# I# ^Neurons from Rodent Brains
: c% o- n/ G, t9 ?" ^. T$ o$ d4 ^. Q6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
7 _1 G( |; T( G9 j1 hTechnique$ I+ e2 ^0 @, E4 R1 V% x
5. Section E Germline Stem Cells
* l( I! \& S4 q. j% g& ^9 y1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
5 q6 M3 ~% R1 E/ i2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
% ]% b3 d. d0 O' {% A6. Section F Gut Stem Cells
5 T. H4 P. K3 |5 h1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells  J8 s8 t/ Q5 b
7. Section G Lung Stem Cells# }$ i6 [( {1 e  P# i! [
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
% i0 ?' z2 j, }- r5. Chapter 3 Cancer Stem Cells, H" N$ ^1 T. M' M$ G: v
1. Unit 3.1 Colon Cancer Stem Cells4 ?6 ]% ?) z& {! x: v' l2 `
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
6 a9 Y. O- r* w) }! U$ L3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells; e6 o7 l, C6 d1 o0 L2 c- [
6. Chapter 4 Manipulation of Potency
2 U9 o; H9 L2 x. x1. Section A iPS Cells( _; y3 `9 ~+ ]) ]7 `2 E
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
( f) x& }) c  s1 o3 `8 B* `+ K2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells2 y* b7 ]4 M( {# Q% j
2. Section B Nuclear Transfer
* F' L' s' `6 z: p% X  U6 e6 H1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
/ n' u+ a( j9 ^* N# `' R. a7. Chapter 5 Genetic Manipulation of Stem Cells
; C  J+ N' D* e- O' L- ]1. Section A Lineage Tracers in Stem Cells# _# w' L6 C6 ^) \8 _. n. m6 J5 i6 ?, i% G
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
+ V. }( Y6 z( u# R3 N# a4 f4 b3 V2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing: r1 H5 j+ C& `$ b' M9 b
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
: \7 Z2 O1 P* N; w- P; Q' y8 }4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
8 v3 _4 U, p& r5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian- E3 E! @6 T% N+ d5 h+ ^4 B) X  k
Neocortical Neural Progenitors3 s: H6 Z+ o1 s4 O
2. Section B Homologous Recombination in Stem Cells5 p0 U0 g- b' `" J0 W3 l7 N0 D
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
( w. e" N: l1 _7 v* {& B; f2 UHomologous Recombination
- V& r8 {# h( D* m8. Appendix 1 Useful Information& E. I- y( v7 i/ O* u$ }; |2 K. b
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research0 {0 K8 \! ^) }$ F3 B
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
0 }$ {: [3 Q4 q; a: q/ y9. Appendix 2 Laboratory Equipment) O0 q2 w7 ~8 q! D! S* h/ ^( F9 x
Standard Laboratory Equipment
4 `# Q" f6 Y: ?) a10. Appendix Suppliers
, d' U- W7 k' h7 \$ GSelected Suppliers of Reagents and Equipment8 ^2 I' S3 ~' _9 [& n- o. p

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