PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页
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3 C6 O) j% ?# i& M2 A; t* QOnline ISBN:9780470151808
* n# h4 E( A/ |/ W0 jDOI: 10.1002/9780470151808
% l0 K1 g* f3 hTable of Contents" _' `$ _8 m2 X& e0 I
1. Foreword$ K% B# M# e7 b# _" A) I
2. Preface
/ t7 j+ r: a, {. W/ P1 g+ N# q3. Chapter 1 Embryonic and Extraembryonic Stem Cells( Q( z+ R% u/ C  d( [! T; U
1. Section A Isolation of Embryonic Stem Cells5 R6 w* P  c* r2 t
1. Introduction
6 K  C2 {0 l7 k# n. u. W2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells* g6 J% A/ f# o0 A0 g7 Z; X2 {- K
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts. C( h$ s4 Q' G' ?( n5 n9 S
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
7 z( j2 f0 y) M3 K6 b4 W' L# g& f5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
5 Z, j3 K, s- U- J2 G6 T: S, D2. Section B Characterization of Embryonic Stem Cells
! c0 g# b' u7 T+ Y7 w  Z1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells& n7 n5 M. h" i7 }2 e1 s/ o
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
- T0 K4 }  v5 xEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform6 ^9 S( i$ _9 z# v/ V8 ?
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells, Q9 H* h% ~. m, `: {2 f5 C' d
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of
" H$ [9 D: ?! Z3 v& ?2 ?Pluripotency
; j  R- z, h" u2 T& }! q/ |# j, u5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
7 Q3 m7 U% N9 E: E1 b. i$ dUsing In Vivo Biotinylation" M. [. \; l' d$ s% W
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem6 g& `/ q' l8 N7 R7 }
Cells7 ^1 z" N3 y4 b  J( i
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
+ b( F* x- }3 j" z% KProfiling
- w( r4 a, l( K  t4 I3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
, X" u! \! s. a, W/ ^# Q1. Introduction
; s" Q3 L. h8 g/ Z3 j2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro( s6 {% \1 u) r% U. r' w/ ?
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture7 B8 B% v6 h/ C  M* |
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
) a) E% g. |. l) CMouse Embryonic Feeder Layer Cells- u' d$ W# _, J+ l0 }- W7 J
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
& f7 u9 J7 g1 k4 \/ N/ w5 ]6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic6 H8 W. E( I, ~" s5 x/ m9 |  B
Stem Cells# X2 }0 N3 U! ]( J
7. Unit 1C.6 Isolation of Human Placental Fibroblasts
0 r/ |  h! R. X8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic- s) ^, o, t0 P8 E1 p
Stem Cells
" l* Y: ^5 S2 v) y# r9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of9 P2 y' K% D* ?8 G( x+ A
ROCK Inhibitor
: ]  m1 }2 m! {/ w# f7 s& c10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells$ m1 q6 Q8 |" z) d6 u1 D+ k
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
% t1 T( ]* j& V7 a$ _( nStem Cells on Mouse Embryonic Fibroblast Feeder Cells6 Z( t8 \) v- k2 B; w) s
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
! C% s8 M6 J* j! D4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells$ `: M! i# M% k5 F3 R" e
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
0 g. ~- C. R' r4 J2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by. o/ z# y# Q" Q' L4 n* m5 v
Suspension and Hanging Drop Cultures
5 n' O5 }5 `% C# H, ]- K3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
- T1 n9 \1 o9 q$ land a Description of the Hematopoietic Blast Colony Forming Assay) S9 H0 O  g* k# ]+ G0 K
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically. |, n  m# ]% m, k& L6 O+ o0 J
Defined Culture Conditions
3 c4 E, {. Y6 B) N# I/ ?5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells3 T! I; |2 I6 c
5. Section E Extraembryonic Lineages
3 I1 G0 v5 o$ w- \$ b( s3 E1. Introduction1 H; m4 S* p+ Y2 d; o( {
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
; p0 V1 j& J0 G2 U5 A5 ^: O; Yinto Hepatocyte-Like Cells
. P5 b. W; o/ l! r2 a3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
% z9 s5 f. D% p, V7 M% {- e5 ~4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells! p4 j6 d8 ~) h# c5 L- v: b9 }7 g
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells+ i* G) \3 `! J6 h+ y( D
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells3 q$ p# r" C) R- y. T
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use- b9 {! H9 [; _6 s8 R) n3 |$ g
6. Section F Mesodermal Lineages
6 Q4 D" {7 w, j+ r1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells7 f% b, x8 V2 a7 H1 ]) {
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture# I  y: O4 s& i% N5 @& y6 t. R
with Endoderm in Serum-Free Medium0 L6 a/ T$ K& o3 `8 Y
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells4 z, m* z" {+ E7 W3 e
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
( t  g5 x2 m/ d0 k: z5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells: L/ u3 X: _0 `; Z+ p
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation' ]* x( W9 G+ A3 a$ l
with Stromal Layers( [" C7 S* W; e% c$ i$ D4 k0 X
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
5 ?; |$ K; q( ~2 kMurine Hematopoietic Progenitors
1 W3 N& p+ }) Q8 K& A7 q5 Z6 l) n# }8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
& u! U# [! o5 K+ M5 m' Q% WHuman Embryonic Stem Cells2 t3 `/ l7 ]% V! A
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells7 A( Z; }( u) _8 H* }( p
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
" h- o( h6 L4 @, d) D% sProgenitor Cells7 ]6 h7 V% O+ O( |2 i
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the( r- u; b3 V+ O2 O3 r9 A
Hanging-Drop and Mass Culture Methods
7 I" P: {; c% V/ ^7. Section G Endodermal Lineages3 G4 W) d7 w7 y* e
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells" v  {" `! d9 r  a2 @, l
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
' J+ Y# Q  z, a0 W3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm& T6 u1 _* u1 Z# u) n
8. Section H Ectodermal Lineages8 @0 A: Y0 k1 ^  X; D
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons& h5 |7 r. ~# J8 |% ^
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by# M! {) u2 Q$ q9 @
Two-Photon Microscopy0 j" ~/ C* U' P* Z# \
4. Chapter 2 Somatic Stem Cells8 J9 u( e* H+ i6 I
1. Section A Hematopoietic Stem Cells
6 _1 a' l; H: n% U7 N9 \: }3 U$ O1. Introduction
9 m8 f5 S5 |3 _" U; r" f2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density6 K4 [" m8 u6 R& W
Gradient
7 q( R5 f6 ~* d. p2 T3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
5 g# N3 P0 L' C! J! [- F5 P4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
* _; J8 u/ O, A0 d4 L5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
; g2 F% H4 o6 u: M% n) R4 pfrom Adult Mouse Bone Marrow
* i1 n8 ?, d6 F" L' \, \$ i6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche; ]7 {/ h9 o! r$ V
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
, |( V9 L/ q. }1 N) l: S. \2 uEmbryonic Tissues
" q& M  G& f3 @8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells& V; T# z4 f! W
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
9 k( F' K: |" I( t2 ^& M) N10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
) [+ f9 {" {/ t  L. s+ Y' y% g0 u3 e2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
% [6 Q9 b* h; J( d6 f8 M1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
1 i. D1 R& c9 C; y! Q6 sTissues
' ]* S- q' v  ^; |% K5 O2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
/ S+ l/ C' \9 @( G+ v2 O3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells# g9 n/ A1 `! X5 S1 K4 E
3. Section C Cardiovascular Stem Cells" t1 J) V; f! [: t
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
' B! J$ i, u0 ?2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium7 B, x' d0 C+ Y/ o& u- Z
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
/ B! i% [& b4 Z! x% f; j9 t4. Section D Neural Stem Cells
8 ]( K2 l# ~# o( a- l1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
7 s. ~  \( o( G$ Y2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
) F# _+ o: F7 d6 j' E* A$ P3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
: u" {7 J4 r, ?7 G$ H% F% G- j+ KCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
& ~! I: I0 F6 ~1 sForebrain Neural Stem Cells" E" x4 |) n3 m9 O- X( ^
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage+ O( B& t( }( A3 B) l/ C
Progression, and Maturation7 l3 @9 k! u2 i; y
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
) y3 P% R7 ]" BNeurons from Rodent Brains
3 o8 R5 V7 R7 k1 G. h6 w3 H0 ~6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
3 o$ {$ C" X8 s2 p; a- lTechnique
) U( [  v$ p' ^5. Section E Germline Stem Cells
+ g% u: t/ h6 ^2 ?7 n6 q( H1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
" P' n2 R% [0 d# s8 q# T' i2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis$ L3 l7 t# e  z+ L4 M0 T5 p
6. Section F Gut Stem Cells
" E0 g0 h0 H$ k% @: g* i1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
. O) H& k: x! F# R9 |7. Section G Lung Stem Cells
. v# h7 q  t, I9 Z# ]1 f& A1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
3 b  |4 b$ D& O. x7 A0 z! A5. Chapter 3 Cancer Stem Cells
5 |3 q5 U; d5 ^' D+ z8 B- d1. Unit 3.1 Colon Cancer Stem Cells# _8 p' @6 ]* v7 p3 c& r# `, A4 e8 {
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model3 c" S; A+ \( l
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells5 t) j: O: A& o/ u3 k
6. Chapter 4 Manipulation of Potency9 v1 d+ T' j4 d7 ]. V% p4 S/ W
1. Section A iPS Cells
- u3 X8 \9 n- G( G6 L8 Q6 n1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
/ W4 D' w0 G1 w5 Z! e( T; C% B2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
5 |( [* f$ M5 u- N3 i* Y! n2. Section B Nuclear Transfer
' j7 o+ F7 d* \+ }7 {2 X1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency5 k& `- k) Q2 V- L) j% A
7. Chapter 5 Genetic Manipulation of Stem Cells
3 P4 t* ]& p0 B5 v; G: F1 Q1 g: \: J# F1. Section A Lineage Tracers in Stem Cells
$ z# A0 n  T3 y$ I' ]" Q9 K% ]8 K1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
. j. |$ M7 i1 T3 w/ G! k2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing5 n: Z+ l4 D5 J" d5 C. C2 `9 D' K
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
. P. x, d: F4 \9 V3 r# y, k9 H4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
  o. f% f7 J3 a5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian9 O2 a: B9 u9 H3 q
Neocortical Neural Progenitors. F( c7 X  _. j* D
2. Section B Homologous Recombination in Stem Cells
) y. @/ X! v  J, o* @5 S: q/ e6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
* M: c3 e" m: _2 l& k' a- jHomologous Recombination
) x6 f% w7 w7 X, f4 p, I8. Appendix 1 Useful Information2 T5 m% L* ?; X5 n; x, g8 b( P
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research" f) F1 J4 F. H* j! b
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
- W3 T1 V* Y4 G) a8 c+ C0 m9 T9. Appendix 2 Laboratory Equipment/ k8 w, M  n* t! T+ G
Standard Laboratory Equipment
& i% v+ N" V% y6 f+ {) o10. Appendix Suppliers
3 N& b, m6 U) }. E2 \% fSelected Suppliers of Reagents and Equipment6 X' @6 M. K' \& A

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