PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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9 n  \* T) ^* b; {# a/ h- z  C% ^Current Protocols in Stem Cell Biology 2007年完整版 1451页
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' @# s$ ^, R% V' }- POnline ISBN:9780470151808/ q/ O8 S& J& ^: T6 K
DOI: 10.1002/9780470151808
* g; m) j# G! l' ]$ L- b7 uTable of Contents
8 {' w3 S& q& E7 F1 y  L5 ~; ^1. Foreword! w& U/ ?4 U9 X$ l
2. Preface
4 I; P' `- u& ]: Y( A- K3. Chapter 1 Embryonic and Extraembryonic Stem Cells( y8 F5 U& K& X7 L' X7 [
1. Section A Isolation of Embryonic Stem Cells
$ k5 @* z' P4 W, v4 C1. Introduction. I" Y% Z5 a8 L+ d8 r
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
2 \7 u  b9 t% V- {; E2 Z, @3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
3 a0 K) ]1 r& I2 t4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells6 r! U+ [' Z2 V6 Q8 a+ W6 d" Y- N
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment  y6 j; h2 S- w8 f' B
2. Section B Characterization of Embryonic Stem Cells" Y' c% z5 S3 f
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
, D/ [; x# d0 C4 n: }1 d4 N. \2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
: S# V# I# d6 T, @" N! r+ R/ rEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform, z9 _, e7 M7 s6 \8 |  A
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
9 Q- y9 ]( L2 L3 x4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of' p; [# L# x3 t: h  n6 c5 M: Y* d
Pluripotency2 G, i' X7 o# H: D
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells. Y0 t) ^) f( ~' m3 X6 z0 p& r
Using In Vivo Biotinylation
' Y0 w5 n+ |9 O5 Q7 ]; v& ?6 o6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem$ \( a& [* C5 ^# q% a+ \' }
Cells9 h+ j9 k- h2 l  f* x& i, g) i
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
; m$ l+ {' W5 ]5 c1 J( VProfiling
0 d+ D" b- a" o& N: F/ q3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells, s) n, Q  T7 v. \
1. Introduction
; b( r8 @: n& N3 A) C2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro( M  J  `# d* S# `
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture: D1 L& \  Q+ s+ ?5 T% g! R5 E
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of  K" j( w9 x' @
Mouse Embryonic Feeder Layer Cells- m% V5 x  b  E( I8 M
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
. S/ F3 y' Z' z3 `) A, d8 a; k( O6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic
7 `" x  ^( b' J/ m. xStem Cells1 g( M4 F( E( ^3 o7 P
7. Unit 1C.6 Isolation of Human Placental Fibroblasts
8 ^8 k9 D2 r4 K6 w+ X. L8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic# p4 g2 q+ t: g/ e$ @0 A: {0 m
Stem Cells; E& B1 ^6 e( i, T' t' E
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of
4 F: O( O, r5 X4 |' R( H0 h# TROCK Inhibitor
( l$ O  |- B$ Z  m6 L. `10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
% |7 t5 ^4 I2 a/ a+ |; s: K8 v& M: L11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
+ E' c0 T" r, g, q% ^; N* n) m# mStem Cells on Mouse Embryonic Fibroblast Feeder Cells
4 }# `4 F) \# Q  U12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers) o$ {' f7 E7 B1 X2 a
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells# ~& j# z( I1 }( U) ?3 H
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap. }5 B+ t+ s! ~2 Z1 ~/ {9 j
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by' ?* n/ R9 m5 ^
Suspension and Hanging Drop Cultures
/ R, H: D  S6 k: L3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
3 Q8 {+ N" W) M6 |. Mand a Description of the Hematopoietic Blast Colony Forming Assay
$ \7 H8 o- N- G% m7 u5 W4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically! z( E, i7 s) I( c3 r0 g6 d
Defined Culture Conditions
: X, M2 a, |% M5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
2 t3 |$ x+ q+ u% ~# R; l; [5. Section E Extraembryonic Lineages- D6 \3 y  `% k6 B6 ?4 ~, [
1. Introduction; P2 l" d3 ^/ Q( O
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation3 z! v: O( i: }: @; ^" x
into Hepatocyte-Like Cells, c9 l( O' `8 @0 q1 o
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
" r' n0 p7 k, [8 F4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells# b! y8 E  A1 G) P, E  `" E* M; r
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
5 o6 ]7 G. h9 w* O; a6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
  N# _6 G( `/ w* I9 ~/ T8 c7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
! i( [3 V4 q* U0 T: W6. Section F Mesodermal Lineages
; S! G/ `7 A; F1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells4 T5 l4 [( z2 z* Y; V4 z6 _( d
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
* s4 `9 G9 ~) ?  N, }$ Cwith Endoderm in Serum-Free Medium
' f0 R7 F: k3 C- i& x$ Y3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells7 f. ]$ y- E2 v6 q9 g6 u
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood+ ?* L- H8 o; g, U/ A4 R4 m
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells, V  z" |3 @' u# y
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation
, ?' p! w0 D0 m/ T, k4 ]% B: I2 Wwith Stromal Layers
, u  P3 K, H7 e4 D- H" n7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
2 L1 v$ D; i2 t: jMurine Hematopoietic Progenitors; O  u& U( F* R$ {) b
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
' H0 a7 r1 d7 Z" V7 O8 cHuman Embryonic Stem Cells' }# g7 S5 y0 F" v5 J, ~
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells; @& v( P1 P) S! h
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac7 D) t  p0 a) [- S. \# W2 f
Progenitor Cells: I/ v2 R) Q% ^8 O8 L$ S3 o
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
) X1 m/ j$ m& }( P$ e* \. wHanging-Drop and Mass Culture Methods  }1 [/ N" M) k5 P  J. Y! Y+ B
7. Section G Endodermal Lineages' M9 \# O- U$ ]3 j
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
4 y  K: t5 c7 O: U9 L/ U0 |8 M2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells9 \/ f) J9 v, i/ N
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
7 F4 Z% ?6 W9 J, @9 N9 K# P) a8. Section H Ectodermal Lineages
/ }- C& [! ?$ Q% z. U2 E1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons6 ~- m& T; Z4 r( R- I
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by
, Y$ u. w2 j7 m3 o1 p1 ITwo-Photon Microscopy
/ {/ w  N1 Q* f6 E$ h" b* y4. Chapter 2 Somatic Stem Cells
. d% j  Q. g5 e7 V1. Section A Hematopoietic Stem Cells
/ |" r/ G7 O9 Y9 x- Q1. Introduction
' s+ O9 @% F1 A" |' v2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density! L/ V( A: t- X, W, }! R, r- b
Gradient
' H; U" |" E9 H. z( W' n3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood/ u- W; T/ k/ K& Z) x* G
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
! G  o; T5 c) z- P$ b+ `; S3 V5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
) l: X/ d2 W8 }from Adult Mouse Bone Marrow; E: ~/ n9 F$ \. }& u" @
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
  G6 o) }: e! d! @7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine* s3 j0 ^% T/ x& d
Embryonic Tissues
9 g6 W$ K1 r. I+ \1 ?8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
1 Y" L7 b/ [' U9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells; l2 m! z  Z0 \+ {, l
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta% w! g# g2 T* I+ p0 U
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells% D- [) |: E% M$ I1 u8 h2 b1 L
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
+ M* ?; b- V, j" \* D! |: i) `Tissues1 l7 `7 p+ D/ K. v- T$ L: ~" l% ^! Q2 r
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells  C. T4 p# C$ P
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
" d9 C) S, d. T# `3. Section C Cardiovascular Stem Cells
) H5 b) R5 y/ z+ X$ {1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood- v( J0 H' d  v7 ]2 B( M$ a4 ?" g9 o
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
: z# I8 C2 C- t! W3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
1 p  D9 T: H7 f+ W* j3 Q+ _4. Section D Neural Stem Cells
) z, ?: }0 D- E1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
, A; S5 D  U* |/ ?- x  M9 P2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells' ?1 d- d4 w; g9 J3 J% p% G
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
: k$ T- m! m; i- s/ E& KCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
8 u. ^. w0 j+ VForebrain Neural Stem Cells4 ?, L1 x7 |) ]
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage" [" W9 F; P2 S3 T1 q
Progression, and Maturation1 L% v  s  F5 \5 `, ~8 K
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
/ S; _* ~- d' H3 w  T" Y6 |& gNeurons from Rodent Brains4 ^* P$ K! ]& q1 y3 H
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere. q( h& g2 q6 V4 |* \# T
Technique
2 F  a: ?6 C/ o0 t) C* [5. Section E Germline Stem Cells! F' ^6 w( W9 R6 k* o
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
* \2 N6 N+ g/ l5 O2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
7 {0 O/ Q$ e5 y. h# a# m6. Section F Gut Stem Cells
/ P. C! |. k# U$ g9 d3 |% a1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
& H: J$ i$ T' r0 F3 Q* K) P7 h7. Section G Lung Stem Cells
' ~1 B9 {# A7 `1 t1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
6 e! a( B( V2 w: n5. Chapter 3 Cancer Stem Cells
! s/ o8 Q( T! V& P# r% J1. Unit 3.1 Colon Cancer Stem Cells" e4 L6 q4 Q! d) [
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model- L" I, X) W8 \5 m4 q
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
( f4 ?8 N# x: A% x7 n* R6. Chapter 4 Manipulation of Potency
# I& p& l% W9 r: H4 s1. Section A iPS Cells
1 V/ K+ n. @& L" H9 t0 ~1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming7 L4 x1 Y4 y4 Y# s0 L7 M2 {9 U" f* o
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
$ D1 r! j) T5 ~4 ~2. Section B Nuclear Transfer; T* [/ d0 H* \2 M' h" a
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
8 _( ~& _, T9 G2 V, ~7. Chapter 5 Genetic Manipulation of Stem Cells
4 X% F# U; i4 [1 j3 e( ?. ^1. Section A Lineage Tracers in Stem Cells
9 F. g9 Q0 _( n1 L" b  }$ w: Y3 L1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma% c$ f  J5 M$ \4 h0 J
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing6 E2 z, w) N9 ?# p4 C, Y
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
4 f! ?. O. n1 T3 L4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
  K& D& a; X6 A8 |0 _+ A- w4 r0 `5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
/ V3 K) D3 m( W% G) rNeocortical Neural Progenitors* s. F8 n, w! ]: \( d3 \
2. Section B Homologous Recombination in Stem Cells
7 N0 j6 A0 E% u8 ]- O6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by- ^( |/ b, l: x* ^/ ^
Homologous Recombination( q( K! z) A5 T+ K% K$ ^  }, U
8. Appendix 1 Useful Information3 y2 ^6 h" L9 G
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
! Q6 O5 y9 ]9 _, x2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells$ f1 J7 u5 W. P! `2 M0 A9 I% K3 y
9. Appendix 2 Laboratory Equipment& D, C: u: f0 ~3 N. N* ?
Standard Laboratory Equipment
7 y  ?( i1 P% a( n7 \10. Appendix Suppliers" ^8 \' S: n4 E, R: ]8 R
Selected Suppliers of Reagents and Equipment
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