PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

cz200203

' E2 s6 _+ a: L% R. x
9 S( h7 O' R. w4 g6 d; |Current Protocols in Stem Cell Biology 2007年完整版 1451页
/ ?$ t+ t# W& l& M+ W: A. j# Q# Y$ M0 @" j! L
Online ISBN:9780470151808
! @% H7 T3 H7 _3 t' Y& {; ODOI: 10.1002/9780470151808
" `7 M1 C4 M7 h4 M2 }% q  p6 S* DTable of Contents. Q5 A% }/ {$ F9 t% m) y% d: x
1. Foreword8 B, Z7 [' ?7 Y; {8 K, ?' _
2. Preface
  E) m& S6 d: a1 s9 w1 j3. Chapter 1 Embryonic and Extraembryonic Stem Cells0 X8 O) J" _6 p& G& r
1. Section A Isolation of Embryonic Stem Cells
$ C) v5 J0 Q& O: f1. Introduction5 j( }5 r5 P! W+ }  O" z
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
$ l/ N- R7 h8 K3 m9 k8 U3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
/ q9 z, o, c- [: J& L8 D- I- p4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells6 k6 x! H0 _. Z0 x: h$ l0 y% ?
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
% j- N1 Z, h! G2. Section B Characterization of Embryonic Stem Cells2 D. @) y# H7 [' N. j
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
* p+ H; b: j3 a* c4 r7 D' F' V$ T2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and' n7 ~6 z7 x( e- a
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
+ h8 \$ U* w9 X3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells5 n1 f# l2 Q  H- R
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of
7 X3 V% V7 c/ K: [$ f  yPluripotency- P% H9 Z  g2 S" r
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells4 j. p! h5 l1 O0 E' c, k9 Q$ W
Using In Vivo Biotinylation
+ _3 D! v2 {, ~2 g6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem5 y% L( [6 T8 O4 d
Cells) l3 u" o: b% k; c
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional4 P8 ?/ w. L6 g( H) }
Profiling+ r. L+ h; B! H% @2 ~* A
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
* ?" s! p- p- X$ K. ~8 O+ C, }1. Introduction
( [8 l: m5 H( ?2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
/ Z; j2 l8 ?! Z' N2 r3 m! ~3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture: ^) s' D  ~' C  K
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of6 P$ r: L0 r$ R8 V0 l
Mouse Embryonic Feeder Layer Cells
+ m( r. u* _5 k; u5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
, t; `( _3 f- H  b& G9 I, |5 l6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic+ |7 F0 B/ w8 M$ u7 i7 h
Stem Cells
' E5 w& ^& z7 @% A7. Unit 1C.6 Isolation of Human Placental Fibroblasts
% j+ B% ?4 p; J% W9 E7 z: `( D. q8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic2 ~  i' \( V' [1 k. c! ]. \
Stem Cells
+ Y1 y* R( V$ Q- ^: d$ K$ H9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of
) L! u( q& m2 L( p8 x! s8 K$ `% hROCK Inhibitor' p) _2 u8 W1 h
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
# p) e' f; w* g: M: u11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
1 t3 d  J! D6 e  r( b4 ]2 ^Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
) V; m, q$ d( \2 U; ?' t12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers" U, W9 p4 j/ c5 a9 V
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
% t7 w/ o/ s$ b6 [1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
  g: N& t1 R* [& L. r2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by9 G& }! y3 `8 ?5 F2 E& \
Suspension and Hanging Drop Cultures& X0 P/ D3 F8 R
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
' i% a2 J7 I9 ?/ W( A9 _2 Band a Description of the Hematopoietic Blast Colony Forming Assay
8 E0 X7 Y+ y  z) x4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically  F& l2 @  ~+ O% \' R
Defined Culture Conditions' E; I' i, S0 R! H& |0 C2 }  {
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells4 a% G, x8 H* v4 N
5. Section E Extraembryonic Lineages/ x- \. `3 D" u+ A
1. Introduction/ |  z% F1 L7 i
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation) c, f. R& L' S% a
into Hepatocyte-Like Cells
7 A* q4 z; n" Y3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
8 W8 x7 J3 [8 L# H$ D5 Q/ E4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells9 Q; X2 W' h/ g
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells% p' M1 g2 e) R; \. O6 E/ y
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
( c+ x% A) {' n5 z! _$ [6 j) p7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use2 f) o2 }* X- W4 b
6. Section F Mesodermal Lineages
8 |. S" S! d: C+ v1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
" z/ J0 n( Z: f+ E$ g! |' e2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
. o! d! D6 v( |1 \with Endoderm in Serum-Free Medium
' ?  E8 X2 I. U6 b  @! s& C3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
% z& v$ b! G, B0 D4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood: y/ u: i% e0 c( T
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
3 H+ Z  s- O  u/ {. L6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation+ q: e" s0 h# y6 c/ Q
with Stromal Layers( d* o" P& k) v. _5 d
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary! y3 c- e' Z$ R0 n
Murine Hematopoietic Progenitors
& v# i; E9 \; W) Q8 ~7 o2 ?8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
/ j+ _0 R! X" \Human Embryonic Stem Cells* ^% S( b2 U# h; u) u
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells3 y7 K; |0 @9 P& g
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
( J' M8 P7 x! o$ P, T- qProgenitor Cells
, D) @) B* t* f" y+ \8 g7 r11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the& x' c  y1 D9 ?- c
Hanging-Drop and Mass Culture Methods
# C/ E- U- S9 u1 s, }7. Section G Endodermal Lineages" J3 Y% g6 i" N! g
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
( @7 ]5 y! E+ X+ n0 a2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
& m, J' u4 Z. Y0 M! D2 {! n6 O3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
9 e. i1 i9 m! r9 `  \/ `: ?8. Section H Ectodermal Lineages
* O) P! F7 {. u- h2 [1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons! c* P+ Y" O' X; ?, {$ o
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by% j- v" r6 n8 k' H) ]5 }
Two-Photon Microscopy
/ Y) K& D( c' O5 K* q" l: h4. Chapter 2 Somatic Stem Cells% \# |( S, X- @% B
1. Section A Hematopoietic Stem Cells
+ v" m8 A- A8 Q5 q1. Introduction3 e5 M- W/ o5 V. ]/ S6 d
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
9 S9 o* e9 p) o2 a" ]; \7 QGradient
8 W1 D9 ^4 Z8 |3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood, h, k; t+ I, x* i4 e
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
0 M' C2 t1 S" X5 S2 z5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells+ V9 F; L' A" p8 S! V
from Adult Mouse Bone Marrow
  ?2 ]5 @2 S1 Q7 ]6 P6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche9 M8 e6 u, M7 g6 I. h2 m) b' B
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine8 {& \; q, n( C' t! r
Embryonic Tissues5 E3 b1 Y  g& v' B4 p% {9 v
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
: s+ g$ W8 s9 F& Q; j# l9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells) q, q$ S; y  S  T0 T/ u; t) [
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta# ~; o+ M+ ]) t% l/ h0 h
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells; ^! a6 q; U! s# \5 e- C, K9 z4 m2 h0 q, Y
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
: V+ v' A9 R3 G! k) P! JTissues! V( m1 a* A- t
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
0 K# i  m4 L) T: s  r/ u3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells9 L( U9 G/ [- {4 M7 J1 H0 t
3. Section C Cardiovascular Stem Cells
2 }" F% j6 c  g/ C1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood' e) m! Q- L" C* |1 e( C* a
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
- Y' S1 O5 Y1 v( t6 d6 K  s+ t$ O3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
  C) M' S5 u2 E5 i& A+ `4. Section D Neural Stem Cells$ I, _. I  K$ {, L& I  n
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
5 B1 J) g/ A3 k2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
0 q) @" x2 v1 o8 z6 I3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
% P) L" @1 W  x' R# ECharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
( G( L8 H  e- L9 J$ d( L7 iForebrain Neural Stem Cells
' O) T- c; O, ^3 F* \4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage9 m8 w4 N- S, r# L6 g
Progression, and Maturation  ^. d& Q# ]$ I6 f% }3 Z( m
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
$ ]1 z- N! q, _" R, k1 s  BNeurons from Rodent Brains
7 L% _5 y# ?6 u6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere" Z9 W/ P6 W% ^3 W# T* ^
Technique
/ k; F7 Z  }: I5. Section E Germline Stem Cells3 p. G6 ^0 Y' @7 {+ a
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila4 @  j: w- O" \$ ^; c- e7 {
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis% O6 P5 O5 \& v/ F7 g8 t
6. Section F Gut Stem Cells
% b; S% {5 H% S: j1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
, |+ v$ y2 e4 l5 H7 \; B7. Section G Lung Stem Cells# g+ z! p6 }2 h6 y/ g0 }
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
( H/ q/ `( V/ {( a- g, \* W5. Chapter 3 Cancer Stem Cells" i# a5 S+ s9 G) ~! A" f! Y3 F% f
1. Unit 3.1 Colon Cancer Stem Cells
& c; `* [1 z( b5 a7 W  H2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model) a- x( A6 B5 V7 \
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells/ c' W; q7 I, u  j
6. Chapter 4 Manipulation of Potency- x% G  p* p% O- t0 l, Z0 g+ Y
1. Section A iPS Cells: p# E5 t$ I- Q* j( x7 J
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
2 }7 G+ w1 |  s8 i8 ^2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells& J, O5 [0 z4 [- W' I9 x3 P: [
2. Section B Nuclear Transfer
1 g& F( l2 T3 |! r, |2 P3 s1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
- I/ C, S0 {0 g0 E8 |1 u1 `7. Chapter 5 Genetic Manipulation of Stem Cells& U9 r# {) E1 @  k/ E
1. Section A Lineage Tracers in Stem Cells/ F/ y7 n( \# R$ q7 k
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma# o( ^( n6 B8 u) l
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing0 l* V9 K- Q! }
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells6 g- ~8 l: Y8 ]8 d
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
$ Y) c( r% q- v+ p3 a, I$ G9 q5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
: O" n6 B0 A9 Q2 T' \+ Q- aNeocortical Neural Progenitors4 ?% W# B1 y3 a" Q" E! S
2. Section B Homologous Recombination in Stem Cells
: h, r# @5 a+ x0 K. k3 b: h  A6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by, E( x$ d2 g- z2 b! i% C1 n
Homologous Recombination& G3 d* t7 X. a3 K* W9 i$ g" ?( d
8. Appendix 1 Useful Information) C, E% B! S. t  D: f$ L
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research2 A/ v* @" r, j( ~* b+ T9 u& C
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells9 k% C: I+ s! M3 V6 x  |) Q  V
9. Appendix 2 Laboratory Equipment5 M. z6 O6 k1 ]" }( j
Standard Laboratory Equipment+ @! L# w5 i! O+ i0 B
10. Appendix Suppliers
% t3 c  T0 m' U( g; LSelected Suppliers of Reagents and Equipment. J8 ?, ~: @1 t4 ~
0 n* V6 _/ f5 i* u

游客，如果你要查看本帖隐藏内容请回复

张佳

知道了 不错~~~  

biobio

谢谢分享了!  

marysyq

好帖，有才  

s06806

很有吸引力  

ringsing

呵呵 都没人想我~~  

科研人

楼主，支持！  

keanuc

干细胞治疗  

剑啸寒

干细胞从业人员  

墨玉

谁能送我几分啊