PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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  k9 |" \3 Q3 p1 a8 _7 nSpringer MMB Vol931 2013 共29章 552pages  k8 `8 L, v( ?! r# x+ [. ?. I2 z
Cell Imaging Techniques  Methods and Protocols
7 @& A3 i6 M* `/ J$ S- O# bSecond Edition" ]& z' t2 E1 c, _) D
Edited by& e7 B+ {# v. \; s9 R
Douglas J. Taatjes
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Contents& s1 g" q4 Z: N
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii( G$ ~3 p. u: t
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii4 z2 J2 z1 _5 A
1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       1
/ F- @0 j$ B0 Q0 L: m7 `; B% pDouglas W. Cromey
9 b8 k) D0 T' c2 g1 ]2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29
: ?, [$ B+ ?3 y, r5 u8 PDonna J. Webb and Claire M. Brown" _4 d$ e- |: E0 A% X
3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   61
. u! s# R& o: }7 CJ. Lacoste, K. Young, and Claire M. Brown5 e* Y; P# g" }" r
4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic 4 W* W8 N5 I' }' b# P5 x
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
7 s2 @: J9 \+ Z% E8 W, d/ N% |& TWinifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
( ]! {7 G, n$ R  i( j5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97
' m, o/ E4 b  f* nJeremy Adler and Ingela Parmryd" r$ O" S9 E( r( T1 K% O
6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    111
2 Q7 m; V0 {" p3 @+ qSunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
3 g# i2 X% f( F* H: B7 p9 }7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
, d: c: G' }$ B3 g; fRaju Tomer, Khaled Khairy, and Philipp J. Keller
; N7 G; P4 _8 Y0 M8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
& i7 o" _- }! n$ U; B; [Lasse Evensen, Wolfgang Link, and James B. Lorens; c2 @" r) ~6 b7 W* K
9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153
! j! `1 a; m& \! {9 j' u. EAndrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert" [6 Y8 v5 r8 V7 b4 o. m4 b( t
10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
* }( U% w8 a, K' Z( O2 d% n( @Interference: |+ a# S4 f9 }, k
Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169
# b5 R5 ]8 d" v* A  j$ n) _/ OWei Sun, Lehui Xiao, and Ning Fang
4 O! }0 |$ _0 N  ^" v11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187
5 F* D2 w9 X0 L% nPiotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
9 |: [  e9 z5 H: R' n2 Z" ^: F12   Laser Capture Microdissection for Protein% `- U1 c( _: Z
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2133 i- C9 ~: y9 c- S
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,
5 g% E/ g' q5 @, b4 s2 r7 v; MLance A. Liotta, and Virginia Espina3 X9 f1 o' n3 c. N
13   Viewing Dynamic Interactions of Proteins and a Model Lipid2 [6 b) o' E; p7 c" E1 Z$ Z! K) k
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259
2 u. T# p. W( v( @Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes- Y' @$ G5 q  s5 S& C% f, U- v' I# b( f
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .2950 D5 e  ~8 L5 k! w" v' w5 r
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
4 l1 F# s8 L0 ]( I! m4 a# N7 I! A; D- ^$ h15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313$ v+ P0 M& u" {2 g6 |
Joanna L. MacKay and Sanjay Kumar
) T6 \3 X) |, X16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
8 C8 b2 p' g) f9 }3 @Lilia A. Chtcheglova and Peter Hinterdorfer0 A  j& l" o% l- L  `$ p, \
17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345
* J5 Y7 }/ ~. ]4 ?. l  SBhanu P. Jena
$ [- _6 b% f% }4 g18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    3677 S' b7 Q- L: o7 Q& B  i, x9 a+ {
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs
( _. L: _( Q3 R' i8 b7 O3 ~19 A Novel Combined Imaging/Morphometrical Method for the Analysis" f) A# n8 h; ?# F7 m( K( P
of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    3918 c) ~# n/ `9 J7 C" @: n
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes* F( {: J( }0 j: m  O
20   Correlative Light–Electron Microscopy as a Tool to Study
5 d$ m$ W0 q" S0 {In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413; I% Z" R, U, u/ }! z
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
  F# J, ]8 f2 Y- ]1 J' H21   Photooxidation Technology for Correlative Light
; u* H( b" s# {8 H) G* Pand Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423
6 w. e9 h# H5 V' F! R$ j) ?' WClaudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, 1 ?+ i/ \, D! ]' b/ R
Margit Pavelka, and Adolf Ellinger$ T; Q* D$ v' I7 \
22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437& b. R- h( k" P; D
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and
0 h! A  y* C8 x  {) {9 G  X$ FMargit Pavelka
  Z; R  s# z! @+ }6 X9 w6 L, D23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     4496 M, g' W& g7 K7 s1 O# X) P
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori5 ?+ x. ]& Z" e
24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
+ t; h, j# N. m$ d+ P# |1 n) {Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader8 U0 x' A; b7 g7 [/ @
25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     483
( u  t8 P7 K: _) q$ PMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
5 s  m, ?! D- h; O0 R8 ]; A26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    493& G) \- w: y* U: G( X
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald; ?7 U1 T  a4 o6 M2 A4 w/ @
27   Environmental Scanning Electron Microscopy Gold' ^1 |# i( j8 J. w6 Y
Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517
1 M# T: f' H3 Y* \( g; ?0 u! LFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
5 [) U; F# f" g, x9 M0 h+ Q4 F9 B28   High-Pressure Freezing for Scanning Transmission Electron
' v8 ?9 q  j& J- _! F8 O$ YTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     5251 ?7 @$ N1 U& l7 ^* m
Paul Walther, Eberhard Schmid, and Katharina Höhn0 ~9 h5 ?0 S0 M  W7 I
29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     537
1 j0 j3 D/ x% i# MStephan Meding and Axel Walch
8 d7 m# M( o: a, O+ c$ h# mIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547
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