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Rapid Attachment of Adipose Stromal Cells on Resorbable Polymeric
* B& c& p& ~. _; f; P! uScaffolds Facilitates the One-Step Surgical Procedure for Cartilage and
' |6 V4 [0 D }* D7 X$ l0 E' F rBone Tissue Engineering Purposes
. c6 v0 s( u" C" z* B5 ~Wouter J. Jurgens,1,2 Robert Jan Kroeze,2,3,4 Ruud A. Bank,2,5 Marco J. P. F. Ritt,1,2 Marco N. Helder2,3
8 ~8 W: A. N0 P% @, I1Department of Plastic, Reconstructive and Hand Surgery, VU University Medical Center, Amsterdam, The Netherlands, 2MOVE/Skeletal Tissue8 ~; B+ k: o) [. R- G
Engineering Group Amsterdam (STEGA), Amsterdam, The Netherlands, 3Department of Orthopaedic Surgery, VU University Medical Center,
, F) T* V) d7 k3 {+ s1 Y6 k2 @* kAmsterdam, The Netherlands, 4Department of Oral Cell Biology Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam5 I" _7 t6 @ f, ~# f
and Vrije Universiteit, Amsterdam, The Netherlands, 5Department of Medical Biology, University Medical Center Groningen, Groningen, The8 D$ M; ^: d6 j. E+ T3 R2 V- |
Netherlands
3 F3 Z- }; e! |, r6 Y0 h" BReceived 3 May 2010; accepted 8 October 2010
" I$ K; C# {' `0 c9 {Published online 18 January 2011 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/jor.21314
0 {+ G N% }# Q% S* Y lABSTRACT: The stromal vascular fraction (SVF) of adipose tissue provides an abundant source of mesenchymal stem cells. For clinical2 D1 o) R& w( }( |( A5 O, l
application, it would be beneficial to establish treatments in which SVF is obtained, seeded onto a scaffold, and returned into the patient
% j0 F: B# b$ G! H1 g# Z; N( s4 fwithin a single surgical procedure. In this study, we evaluated the suitability of both a macroporous poly(L-lactide-co-caprolactone) and a
6 S! s, ^7 e; H" x8 N/ `porous collagen type I/III scaffold for this purpose. Surprisingly, cell attachment wasrapid (10 min) and sequestered the majority of adipose
( _6 H5 A( F/ ^+ k0 n/ Y) Y" kstem cells, as deduced from colony-forming unit assays. Proliferation occurred in both polymeric scaffolds. Upon chondrogenic induction, upregulation' I$ v @5 p! Q" {$ w" c
of chondrogenic genes, production of glycosaminoglycans, and accumulation of collagen type II was observed, indicating2 R5 W+ A; w. M
differentiation of scaffold-attached SVF cells along the chondrogenic lineage. Osteogenic differentiation was achieved in both scaffold
/ _+ w! |; h4 |5 B8 S/ J. c+ a4 ^types, as visualized by up-regulation of osteogenic genes, increase of alkaline phosphatase production over time, and accumulation of bone
7 P+ u) [6 D+ Z' i7 ^5 C$ Zsialoprotein and osteonectin. In conclusion, this study identifies both poly(L-lactide-co-caprolactone) and collagen type I/III as promising$ t3 ?6 C1 f7 t
scaffold materials for rapid attachment of adipose stem cell-like (stromal) cells, enhancing the development of one-step surgical concepts3 C2 D9 n" }4 h: k$ S
for cartilage and bone tissue engineering. 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:853–! W4 [9 ]6 C, ?% M9 m3 h: k
860, 2011 |
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