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Rapid Attachment of Adipose Stromal Cells on Resorbable Polymeric
; H; j/ D1 k X7 H' N% IScaffolds Facilitates the One-Step Surgical Procedure for Cartilage and" O2 s% p% i; z k9 G4 T0 h- K3 ]
Bone Tissue Engineering Purposes" w. E; y$ }0 @" D, A0 p
Wouter J. Jurgens,1,2 Robert Jan Kroeze,2,3,4 Ruud A. Bank,2,5 Marco J. P. F. Ritt,1,2 Marco N. Helder2,3
. z/ f9 D4 [6 L. l1Department of Plastic, Reconstructive and Hand Surgery, VU University Medical Center, Amsterdam, The Netherlands, 2MOVE/Skeletal Tissue* `- W' b% z; ^9 w
Engineering Group Amsterdam (STEGA), Amsterdam, The Netherlands, 3Department of Orthopaedic Surgery, VU University Medical Center,( R" `- P) {" T
Amsterdam, The Netherlands, 4Department of Oral Cell Biology Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam
0 @% h3 i. X/ P) {8 d2 f6 qand Vrije Universiteit, Amsterdam, The Netherlands, 5Department of Medical Biology, University Medical Center Groningen, Groningen, The6 R4 @0 B1 `. \' {6 n# [
Netherlands7 C# G6 Y& m1 \/ g0 }( J) X' T
Received 3 May 2010; accepted 8 October 2010
4 X' ?$ D2 o$ ]' g4 JPublished online 18 January 2011 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/jor.21314
7 \# V! D; e2 iABSTRACT: The stromal vascular fraction (SVF) of adipose tissue provides an abundant source of mesenchymal stem cells. For clinical5 h( [' I8 ~& {* S/ _2 F; `# [
application, it would be beneficial to establish treatments in which SVF is obtained, seeded onto a scaffold, and returned into the patient
4 z- e" H/ K: g% n( O* V7 L: owithin a single surgical procedure. In this study, we evaluated the suitability of both a macroporous poly(L-lactide-co-caprolactone) and a
# A4 {9 z9 ]% j& Sporous collagen type I/III scaffold for this purpose. Surprisingly, cell attachment wasrapid (10 min) and sequestered the majority of adipose) _( j2 ?( K' \! z* }
stem cells, as deduced from colony-forming unit assays. Proliferation occurred in both polymeric scaffolds. Upon chondrogenic induction, upregulation2 Z6 c1 w' n- z% o2 D: ?
of chondrogenic genes, production of glycosaminoglycans, and accumulation of collagen type II was observed, indicating
% R( u" `; h; G4 O6 Wdifferentiation of scaffold-attached SVF cells along the chondrogenic lineage. Osteogenic differentiation was achieved in both scaffold2 b. s( B, |8 M( M2 _. d
types, as visualized by up-regulation of osteogenic genes, increase of alkaline phosphatase production over time, and accumulation of bone
* L2 f; T y' w$ y" a6 _sialoprotein and osteonectin. In conclusion, this study identifies both poly(L-lactide-co-caprolactone) and collagen type I/III as promising' W% R& M _4 z( ^
scaffold materials for rapid attachment of adipose stem cell-like (stromal) cells, enhancing the development of one-step surgical concepts$ w- [6 p4 y) ~" {0 ]. H3 x
for cartilage and bone tissue engineering. 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:853–3 I# x: ~( W* J" t8 @
860, 2011 |
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