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回复 zhen022 的帖子
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9 V4 R m5 C! q* l条件合适最主要的就是培养基,如果你做的是小鼠的BM-MSCs,参考毛宁实验室的protocol即可;如果你做的是成骨细胞,一般要加 sciencell 公司有商品化的Osteoblast Medium 。
3 U4 O B. b7 sProduct Description
& A' T- S! ?% ^$ K$ {Osteoblast Medium (ObM) is a complete medium designed for optimal growth of normal human osteoblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human osteoblasts in vitro.# h3 V& }3 s: q4 S% J# r
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Components
k# m1 T, z) }! t$ _ObM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of osteoblast growth supplement (ObGS, Cat. No. 4652) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
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8 D6 a. R7 D! m( h5 qObM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.' ^6 @0 g8 X3 `2 p* q0 G2 g
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Storage4 _4 k1 O1 u7 k: l
Store the basal medium at 4oC, the ObGS, the FBS and the P/S solution at -20°C. Protect from light.
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* G" A* u. \: T! t; ~+ u0 E% nOsteoblast growth supplement is also available for puchasing seperately
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