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回复 zhen022 的帖子
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条件合适最主要的就是培养基，如果你做的是小鼠的BM-MSCs，参考毛宁实验室的protocol即可；如果你做的是成骨细胞，一般要加 sciencell 公司有商品化的Osteoblast Medium 。: d2 x1 e( W: y T
Product Description) ?' Y& g; [. H* ^
Osteoblast Medium (ObM) is a complete medium designed for optimal growth of normal human osteoblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human osteoblasts in vitro.; d. O" R0 L5 S/ v- R
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Components# Q) Z# r0 @* p" {7 Z w1 r$ a: g/ C) X
ObM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of osteoblast growth supplement (ObGS, Cat. No. 4652) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
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ObM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
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Store the basal medium at 4oC, the ObGS, the FBS and the P/S solution at -20°C. Protect from light.
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. R6 S5 z6 b$ N9 c# LOsteoblast growth supplement is also available for puchasing seperately7 c' ~' X9 C9 {. e& i
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发表于 2013-11-28 23:49
