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本帖最后由 细胞海洋 于 2011-4-27 16:16 编辑
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+ d' t& D0 j" v0 D杂志:Cytotechnology (2011) 63:67–80
$ z! [2 t0 O, i5 f* V& K9 v题目 :Long-term cultures of stem/progenitor cells from lobular and ductal breast carcinomas under non-adherent conditions
( D! o* c. n( Q# d8 O0 C作者:Agostina Nardone • Sara Corvigno •7 q; g* n* \& Q- w9 x3 u; y
Annalisa Brescia • Daniel D’Andrea •
) f. o0 s; M% q- R7 k$ T Gennaro Limite • Bianca Maria Veneziani% \% \3 p1 x, b4 z' ^/ `0 u+ Q7 q; `7 J
文中提到 “To test the ability of LoBS and DuBS cells to form mammospheres, cells were dissociated and seeded, by serial dilution, in ultra low attachment surface 24-well plates in DMEM/F12 plus 0.5% FCS medium (low serum). The final cell dilutions ranged from 10 to 1,000 cells/well. Sphere formation experiments were carried out at least four times per sample.”0 s* e3 d! a9 X* k! n$ }
结果如图$ G7 t0 s- d3 [
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/ Z2 g- ]( k* ^最近养克隆球采用无血清培养基加EGF.FGF.可怎么也不成功。看到这篇文献后有点心动,也想试一试。但血清又有促进细胞分化和贴壁的作用,低血清养出的克隆球和无血清培养出的有可比性吗?6 L+ K) }! ^1 q7 C }2 ]
希望各位可以发表一下自己的意见!; }, e9 i8 ]! N/ m8 i, V
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