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本帖最后由 细胞海洋 于 2014-2-10 21:11 编辑 3 u/ c9 i4 N) ?' }' @& N) k
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Current Protocols in Stem Cell Biology 2007年完整版 1451页
2 R0 d. D' l" L$ `" ?( n6 T" V# l9 A. }) a! P
Online ISBN:9780470151808
" g" @$ g! C% L0 _DOI: 10.1002/9780470151808, P+ n& j8 \$ \9 X9 ]7 C
Table of Contents
9 V" l. J" o4 ^4 o$ Y: [1. Foreword
5 { {& c) i/ N5 B. j$ N2. Preface
; X, U. O% w" |; m# M3. Chapter 1 Embryonic and Extraembryonic Stem Cells
9 T7 f" \: i1 `' o# T1. Section A Isolation of Embryonic Stem Cells
; F& m, p ^: m+ {3 W4 ^& d8 [1. Introduction
* M9 B) Z4 p, {- X' c3 v2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells; P' ~/ c! I/ N' |1 V) c" r! o
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
1 `& u% S7 q' A9 i0 G4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
/ T9 H* E9 }- H5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
4 j, K3 w" M( ]: P$ _) J2. Section B Characterization of Embryonic Stem Cells
8 F4 N/ S: j, x5 E1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
5 h! Y/ r+ @( {/ \+ H1 h. X8 A, \9 w2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
: X o5 Y I+ n$ K# DEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform# j/ ?7 U4 @( `5 @; O# _
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells8 M4 f( I4 ]3 d4 a, R' I
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of
: A" }2 U$ t* r* K W0 ], \Pluripotency
. h7 [* Q, T9 e5 ]2 ~5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells7 e/ H8 K' C) M7 t: y/ `" E
Using In Vivo Biotinylation% @4 O# R: @4 w8 O4 y
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem T2 v' B0 ]2 U4 H+ V" W
Cells# y) }, ]/ B/ |# j7 M0 H. p& y
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional2 Q% c2 i5 Q* [
Profiling
" z& n* f5 R# j, P A9 |) ~: D3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
- L7 S* u% c/ J1. Introduction" F9 M& ?# _% ?7 u/ U
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro5 l- P9 ]0 _# {, N3 S
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
. s* J, H& z; Q, [: H/ m! ^4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of9 g# m4 p. N" s# S: z: E- u$ A9 Y
Mouse Embryonic Feeder Layer Cells
9 D, P: \/ `; P# a5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
' }+ D6 Z: [, k ?3 N6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic0 {) K6 |! U9 {$ [3 |. ?4 |7 B
Stem Cells, U: V7 s' g* ]& O# |7 I
7. Unit 1C.6 Isolation of Human Placental Fibroblasts& s7 x. T3 p$ I& t. J7 {
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic5 }+ Q+ z8 Z* ?1 z9 ?8 K
Stem Cells5 W9 t q1 j _
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of# o9 l- F4 }$ B9 Z' F- c
ROCK Inhibitor$ w' T8 x# I8 ]) Q
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
3 s2 ?- L4 o4 U: G O3 W3 ^& T11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent- X0 v% T' t( B# U8 Q
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
8 t8 h' O- A3 ]8 x12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers. \: H8 Q# d4 B+ z$ T
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells T% b$ d6 g0 n4 I* x* B% P! G3 P
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap/ U; M. b% B) G! O0 V
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
9 a; g- B9 `" g' D& K' B; f/ D+ D! a; CSuspension and Hanging Drop Cultures
0 Q1 G1 f+ y: A; z3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies5 x8 P) q6 T8 A* u1 x% }' \
and a Description of the Hematopoietic Blast Colony Forming Assay5 Q% Y3 h3 x ~% [; m+ b
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically- O! s- W- A6 q9 N# Z1 k% a9 O6 {
Defined Culture Conditions
8 g7 A$ P& \* g. T( C9 u6 H5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
- E* `* a1 W0 U0 i2 l5 o5 U- T/ y5. Section E Extraembryonic Lineages. P. V- u/ `0 ?* d. d8 x
1. Introduction! a) _, y8 Y/ y8 X. G
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation5 P- t3 U! \9 ^" y6 k
into Hepatocyte-Like Cells6 Q9 s2 \* f; p& W* U
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta: p- q9 j% [9 o5 k! x5 A2 @! e* I
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells. W* b. {( L4 t" ~& I: y* v) c
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
5 g( l4 Z7 R: K y/ k" V* k+ u6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
. K' V% C0 V% a; ]$ P8 k( N: ]7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use1 q/ K2 V6 J: D1 \
6. Section F Mesodermal Lineages8 g8 d, [4 F% Z; A9 V
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells) F2 G# `0 [7 M5 K$ f9 x
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
0 y# s: t" Z0 h) c' Lwith Endoderm in Serum-Free Medium) E( `5 L. i) @/ [6 l: `# ^ x% ^
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells4 q" w7 T/ u8 y
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood4 X- r9 |9 e6 u6 d2 Q* G M
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells! N0 e, Q3 e. x* F3 Z) c
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation
5 s" Q! B' Z! f9 ~+ [with Stromal Layers
# Y9 Z4 T! u& S# Y0 E9 R/ Q7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary$ d8 U, V" e$ J3 i. d1 l" L0 Y
Murine Hematopoietic Progenitors; b, s* U m& M5 w1 a$ R
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from- {% W2 Z5 E5 m: Z9 O
Human Embryonic Stem Cells& |* G1 o+ c/ O& z! Y0 p( D& [$ D+ H
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells3 h7 ~) [( r% r+ h7 q! l4 E% n+ I
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac0 y4 z" w! i8 H- `
Progenitor Cells
3 o6 H6 r2 k- Z) F# C6 W4 F11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
% _- v/ U2 j! Z+ A. lHanging-Drop and Mass Culture Methods
: j; n! J9 g$ u1 x7 I: v' ~/ e7. Section G Endodermal Lineages3 d6 d5 U8 j% C) E7 M! V% J
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells3 ~* ^9 T! q1 O; Z; M0 I* o1 \
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
$ \- q1 v* v! h5 ~5 s3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm5 s6 P+ A: T4 \0 e* K5 G5 A% U
8. Section H Ectodermal Lineages
: {/ ?- I1 u7 Y) S# i: P1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
8 p5 g D5 y/ S* g" U, E( [- i2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by9 b( |* ]7 q: K! c) I+ m6 o
Two-Photon Microscopy4 q w* _1 x0 i6 @
4. Chapter 2 Somatic Stem Cells& M' q" c7 D8 n/ @
1. Section A Hematopoietic Stem Cells8 X" h4 J1 S5 [& z" M/ E
1. Introduction
% O% t) x- U# G3 M) \2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
3 D% P. a. q- t7 _Gradient4 O/ g @* S5 {- a* P& f1 R
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
) ~1 [! y( v4 H) Z8 W. H4 u3 Z) w4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
0 A4 o" ^- r% k6 j! t5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
3 @0 a) ?1 g- g9 T# w$ ~+ {) jfrom Adult Mouse Bone Marrow
u5 f; E% m' _9 x+ d6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
0 C: n! @; y: k5 \3 w! d9 _ Q. \7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine! _1 K+ ~$ h8 I- V3 ]5 A7 v
Embryonic Tissues
1 [5 W$ Y1 V7 H4 [9 u8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells% N' T# }& d# l+ ?2 S/ P6 r7 q: l
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
, p/ ^) G+ u9 U5 F r: ?, C, D10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta( @) Y0 t6 z5 r, `9 J
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
$ a @) B# H! p8 l) b1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
5 c6 z2 L1 f, z o+ Y/ NTissues! U2 o% ]' ?4 P+ @( [( T
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
; e) v" P6 E# {, t2 U1 d4 y3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells) ?5 M$ W5 |4 a0 p: T
3. Section C Cardiovascular Stem Cells. u( U2 E' [) U0 a
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood' v5 I6 H& w0 w
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
- \, y3 B+ B- Y# m& Z2 a# |3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
7 O. e* Z C, Q/ y r4. Section D Neural Stem Cells8 q& C( b/ Z$ D/ b4 @& Q$ Z6 l
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains' N( E- z% V6 R
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells+ G: m: n7 l: x0 c
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
& ^9 L- {9 [) l3 XCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
6 _( `6 Q0 x' b* wForebrain Neural Stem Cells
3 b9 j# T! W0 b( T' d4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage, X& }9 b5 m7 z! ?6 e! V. ?
Progression, and Maturation! F5 B8 C0 [0 T* W
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
: g6 ^% y8 c7 _$ e+ E) u7 N' V2 _5 SNeurons from Rodent Brains. k3 I6 v' o- a* V% \
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere- s) a3 v2 R1 l/ H, J t
Technique$ X# s: L& T m" u3 S" ~
5. Section E Germline Stem Cells
' C" B( u" q% h0 k6 C1 [! K3 E1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
J2 D2 N6 a7 H9 z* E+ b8 |- _2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis! |, ^: T; w) q" s
6. Section F Gut Stem Cells$ n8 ~7 p* O4 |" g: r
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
9 _1 H, Q. [7 d j+ K+ R7. Section G Lung Stem Cells; _, S& f2 m: m- h: W& p8 `% l5 j
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells, x( ?- h( t! w
5. Chapter 3 Cancer Stem Cells
* ?3 _- H j% F: |" M! H1. Unit 3.1 Colon Cancer Stem Cells
* j: h+ ?+ L4 O h3 f. e$ j2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model4 E8 l8 t+ D* C4 }1 R
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
2 E) ?/ N$ C+ g: j6. Chapter 4 Manipulation of Potency% t( B/ \6 u! S6 R5 b5 J4 m- \
1. Section A iPS Cells: k; H, x3 m1 ^# z
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming8 B1 l7 F3 L* I0 f6 _" l
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
1 W8 m5 l8 M- S9 H" q, s2. Section B Nuclear Transfer
6 A [: G2 V4 e+ i- C5 Y1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
) @! d: O2 q! i7 d; s4 |( I$ I% Z7. Chapter 5 Genetic Manipulation of Stem Cells
; g7 z" T, {# W4 d& p1. Section A Lineage Tracers in Stem Cells
! D( @9 B/ s/ R' X/ ]6 K+ F Q3 e1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma. m; c+ s7 H D5 n6 G1 t
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing+ C9 \' S1 r* s$ V) x
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
' P" o3 ]# F; |, j6 D% D4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium" n& w w4 D0 a! l$ k! `6 _- j1 P
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian0 ]6 U: C# s5 r* q5 T0 h
Neocortical Neural Progenitors
0 l: Z/ U, a/ q2. Section B Homologous Recombination in Stem Cells
5 O" u* B5 ]( N) r- e6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
5 q* ~( \ w4 F- C" d0 t$ V* bHomologous Recombination
' j$ y; r" `* S1 u. N1 E- m8. Appendix 1 Useful Information
2 X+ |3 ~; d. Q( C& c- J9 u9 }5 E6 `1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
, e+ ?8 O/ Y# @8 k2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
4 r/ E9 a s/ x* z7 `: \9. Appendix 2 Laboratory Equipment
7 z( {- q' p# z5 x# D+ Z3 z6 ^+ AStandard Laboratory Equipment& ^+ j% m9 x/ _: l
10. Appendix Suppliers
2 {1 I" L6 V6 R; b, zSelected Suppliers of Reagents and Equipment+ i( I9 u" E B/ [8 e2 u
+ ~4 U# n4 y, Q: B& e% ~ |
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发表于 2011-3-8 16:07
发表于 2011-3-8 20:14
