PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页
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Online ISBN:9780470151808
$ c. T3 J: ]7 [  x. H. GDOI: 10.1002/9780470151808+ y2 Q& ?* T1 b8 m
Table of Contents
: @" L8 x" ]7 [0 t2 ~/ `; w* A: b1. Foreword
8 Z: a2 U2 `4 r. B1 k+ h9 n2. Preface2 G1 q8 V8 U1 k7 V( q. ^6 l
3. Chapter 1 Embryonic and Extraembryonic Stem Cells
  \( f; o4 S* C3 t4 b% `# \' J: K1. Section A Isolation of Embryonic Stem Cells
" P- _% f+ u5 y* v1. Introduction
6 V2 U& T! \% n3 M2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells6 d" _- I. v5 v' |
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts1 s( Y+ t3 R' a4 A
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells6 e* u) [) G! R3 Z( Q  m
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment: \0 V6 L% n5 R* l2 z
2. Section B Characterization of Embryonic Stem Cells
% }5 {* x; {. t) R: y1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells7 K9 m  [  ?* _0 Q8 S
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
7 [0 k4 b+ v0 {0 m; h# {Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
5 R+ Q% \- v- ?8 _* D3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells9 n: O3 u  f/ n2 E! j& b
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of. s4 O/ P# q/ t0 {" ~
Pluripotency
7 y2 \! D! V& x- O& W, {) R5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
# F+ f& C0 q! H( ]9 m$ LUsing In Vivo Biotinylation/ E& `# h# p& ]" ~! n; {
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem- {5 k1 W! j( \
Cells( O) _) p% O$ C$ \" j8 Z1 L
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional8 N4 E: A& ?* e) u) O( F7 n
Profiling
9 M8 z- N; ^. A- J/ _! @' K3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
% u  C  E" ?% ~, o2 e, \1. Introduction2 N# Z3 R) ^7 V0 Y7 E
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
8 k' n4 @; ]( y/ R* D! {3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture: i# J. a" D* w4 ?
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
2 B  {5 M$ Q- ?# J% d5 v9 kMouse Embryonic Feeder Layer Cells3 O  s% ~& ]- H: s( W: B
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells8 D" w3 `. ~: V1 o- M( {" C; Y: U
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic+ [1 ]% G" ]) R+ {+ s' H2 W4 o
Stem Cells% B, E% d! F! h+ ]- s6 d+ \& h: s
7. Unit 1C.6 Isolation of Human Placental Fibroblasts5 W# n0 [1 a2 t% q' Q' P
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic2 ?  [  g5 @! N# h; T# p
Stem Cells, s; a8 K& L! Z- N) m3 M9 E2 n: |
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of/ u: d6 a# G% v% _) z/ E2 k
ROCK Inhibitor
0 M* ~2 c% q/ ?3 E4 g' \! I10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
! D/ [9 s+ v5 Z' Y) J; n2 h11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent+ a/ \7 _: a1 I7 K
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
* `% Z$ a7 W( D% D) d12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
6 |7 m  y) ~# X/ H4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells7 W+ I( ]! [2 O
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap3 K! g" {6 I4 y: n" h0 h. I
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
: Z' X/ d6 R8 A2 i) N9 a/ f' ^Suspension and Hanging Drop Cultures
1 d& h9 f0 i3 Y; ]3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
. E+ `9 P7 y$ [; S8 M# Z# t4 c2 {and a Description of the Hematopoietic Blast Colony Forming Assay/ X& Q5 h8 H9 V$ C1 w
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically
; f) [0 P6 I8 Z. RDefined Culture Conditions7 K# I" q& s5 ~7 \6 X& n# Q
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
  t  ^$ F5 s3 }' c9 u5 Q5. Section E Extraembryonic Lineages! Y( P+ b. i( e( J
1. Introduction
* m2 ]$ q+ v# O0 M" `+ n2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
+ W  v+ G2 B* e3 g( h/ hinto Hepatocyte-Like Cells& q0 y% ]0 p, \6 J/ G
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
5 J6 |. k0 T- Z1 b! X! \4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
, O( W5 o# x4 p+ ~* \  I5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
' X$ m  E9 i6 }6 v7 D6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
1 M  ^9 y7 i! B9 s! g, e6 U3 s( l' p7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use$ L8 S+ O6 z5 I; a& I9 x
6. Section F Mesodermal Lineages
% c% _6 |, h2 d  g1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
5 B7 p+ {6 s: \; k- Y7 d2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
1 I. A' u. `0 t  M9 d5 Dwith Endoderm in Serum-Free Medium. z5 s+ y: \4 @/ P
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells# ]& X" v7 d4 o" n4 B. O6 X
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
5 _2 T' t% ?: r# t4 o8 [5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells, D0 i- A9 h& H; B" q7 J/ U/ z
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation0 f* Z' z% R6 \/ I! U, s
with Stromal Layers
0 g3 ]  e; t( Z* i/ L7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary% {' H6 \# k. q; f/ ?/ ^
Murine Hematopoietic Progenitors
2 \# E0 b6 z; R% x1 u# v8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
: Y1 }' u. ]4 _- K7 sHuman Embryonic Stem Cells) [( e% K7 J! k. u/ E* b
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
( @0 V2 J, v2 t: P" c9 j10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac% p- I) x& l+ ^0 o) I; ]/ a
Progenitor Cells
8 A2 H, Z' H9 S4 E3 h' ]11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the3 @1 @/ |1 Z) p* p9 I5 N, b
Hanging-Drop and Mass Culture Methods7 b" E8 ^( P: N) j- F
7. Section G Endodermal Lineages
4 o# F8 W7 ?6 h4 i- }1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells( b  B% R  F9 b( p: u; j( w' E
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
& t: B! t& i' M3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm( S" f3 t. o! I: a! p
8. Section H Ectodermal Lineages
$ E( g, a' ~1 V2 e. T% A1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons( z& A7 j; R) w6 \& z. W2 Z- P7 I0 u
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by
" p, U) D( q8 R% DTwo-Photon Microscopy) Z8 n( N; S: p0 ^+ |" z) e
4. Chapter 2 Somatic Stem Cells
) I- }  e  J) S( L6 `% E( V. L1. Section A Hematopoietic Stem Cells
) D* q' i+ J! g9 H* U9 H1. Introduction  J7 j" K  H0 Z5 z0 v) ]( ?
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
: ^8 l8 F! {6 P+ sGradient$ o5 a& c2 L8 E5 V/ b6 u
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood- ^; ~1 S* g: q9 f
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
; h0 U, k' \6 |5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
: x8 o' a, R. ifrom Adult Mouse Bone Marrow5 `4 C6 F1 V  i; r: S
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche0 z! K+ v3 S. Y5 V
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
: K/ X: A6 k% r) h8 @5 U8 `Embryonic Tissues1 g  i4 S9 {9 I
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells7 j+ w0 d: s$ l* w
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells# N3 U1 I# g4 Y" S
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
! q% F, O$ h, L' c, _' q2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
  C  {5 {: C/ M1 Z: d1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
  ]; u; |9 W/ C4 |Tissues
, O& q9 u& O7 Q4 l) @! @% `; j; a2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
: Z- p& y! h8 W3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
' G- P1 s: k( h# s3. Section C Cardiovascular Stem Cells9 L. ?+ h. Y6 K: ?8 b
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
5 d5 ^" |" p6 I; z, Q- E/ m0 Z5 _2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium) Z( }5 Y/ L: s5 j8 q6 L
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells0 M: a1 N, C4 Y% V0 e0 I/ `6 y1 ]
4. Section D Neural Stem Cells
. ]: z! ?! G4 c2 J3 L1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
' O4 j6 L9 ?, V% G  B$ y2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
# {: R* U4 J' @6 c( K9 O. O) K3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
, o' ?* _/ f7 U& g2 c# s4 SCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
3 s+ Q# `" @! C& K; Y: d$ H+ _Forebrain Neural Stem Cells
: {4 O( ^9 t- \' Z4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage
4 y/ U* N6 U  U* oProgression, and Maturation
7 N( X' ]8 V" `- u2 b) K5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
& d  h8 t: f; ~! w  [& C$ F2 dNeurons from Rodent Brains
. H1 ^( ~9 O7 _- M- V  X1 G4 P! a6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere( B5 L( d0 p  o4 P- ^
Technique
5 N' x- J( E5 q( S5. Section E Germline Stem Cells
9 o2 k$ B0 B8 y7 L/ y* L; `1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila5 ~/ m& \% ^8 l5 M7 L
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis# }) F; W) g7 ^* D
6. Section F Gut Stem Cells
3 x, @6 w% _* h7 i$ M0 i1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells$ o9 F+ j+ S4 p' D. K# @
7. Section G Lung Stem Cells# G) s# k" V" S" }
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells& }7 F! p4 K  _/ E/ T
5. Chapter 3 Cancer Stem Cells$ E! _7 k) v2 x6 k! ^' b
1. Unit 3.1 Colon Cancer Stem Cells4 T. b/ o1 I. h
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model: t2 q7 M: g% I9 _& A1 V
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
9 c6 y9 ?$ ?& v& W5 l) K6. Chapter 4 Manipulation of Potency
- U& Q5 K9 k7 a6 u; w1. Section A iPS Cells
& B+ r1 Z3 U) z3 {. v, C1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming4 U: n9 F6 s( ~
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells" ^3 w$ d; P" _1 I# M7 N: q! `
2. Section B Nuclear Transfer6 C3 {4 g0 k: a2 d2 |
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency/ |' C9 y4 [, F: o: D
7. Chapter 5 Genetic Manipulation of Stem Cells# h9 r- ~: X0 b: h' p
1. Section A Lineage Tracers in Stem Cells; b) _2 j: T% n7 D  m
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma9 e) A! ?+ f7 Z; K4 g: w7 _$ B. ?
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
1 L+ A6 i6 F( L4 Y( o3 ^! ~3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
5 g  ?5 W9 K, Z7 k4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium) I, x, {4 k# s& `- x- e* w* v$ l* U$ P
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian8 D& i4 Y+ [7 {! T
Neocortical Neural Progenitors
7 G' K! Q: e) L) A2. Section B Homologous Recombination in Stem Cells/ v  W' i- O4 _) i
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
$ a% i$ y) f0 KHomologous Recombination: A+ e; z5 v9 O0 m* M
8. Appendix 1 Useful Information" {) E& c( F* }& F& N
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research% Z. X" A0 {6 ]3 t
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells& Y+ X$ g7 P# H6 a) Q
9. Appendix 2 Laboratory Equipment
3 [2 q4 O/ {* ^$ D2 V; K% i2 LStandard Laboratory Equipment# e$ d* \+ h' e. l; T  l* h! n( U$ s
10. Appendix Suppliers0 ~/ W. U8 s7 @9 d
Selected Suppliers of Reagents and Equipment
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