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本帖最后由 eley 于 2011-9-25 12:30 编辑
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摘要:通过控制JNK和NF-kappaB的免疫激活,TAK1在果蝇先天性免疫应答起了中枢作用。在哺乳动物中,TAK1的激活又是受到两种TAK1连接蛋白TAB1和TAB2的调控,但是在果蝇中,TAB蛋白的免疫应答作用还没有被确定。本文中,我们对果蝇中TAB2样蛋白(dTAB2)进行了鉴定。dTAB2可以与dTAK1进行相互作用,并且可以激活JNK和NF-κB信号通路。而且,我们还发现通过dsRNAi方法沉默dTAB2的表达,肽多糖可以抑制JNK通路的激活,但是NaCl和山梨醇没有这样的抑制效果。dTAB2的沉默对由dTAB2引起的p38通路激活起到巨大封闭作用。以上实验结果显示,dTAB2对JNK和NF-κB信号通路的PGN激活是必须的。5 f8 C% k# c, _( ` S! v) j/ D
关键词:果蝇 TAB2* z6 }2 e, B1 Z; v. e" T1 `
: U7 W) b9 ~8 V" o" ^4 t! q5 W/ ~ Abstract: The TAK1 plays a pivotal role in the innate immune response of Drosophila by controlling the activation of JNK and NF-kappaB. Activation of TAK1 in mammals is mediated by two TAK1-binding proteins, TAB1 and TAB2, but the role of the TAB proteins in the immune response of Drosophila has not yet been established. Here, we report the identification of a TAB2-like protein in Drosophila called dTAB2. dTAB2 can interact with dTAK1, and stimulate the activation of the JNK and NF-κB signaling pathway. Further, we have found that silencing of dTAB2 expression by dsRNAi inhibits JNK activation by peptidoglycans (PGN), but not by NaCl or sorbitol. In addition, suppression of dTAB2 blocked PGN-induced expression of antibacterial peptide genes, a function normally mediated by the activation of NF-kappaB signaling pathway. No significant effect on p38 activation by dTAB2 was found. These results suggest that dTAB2 is specifically required for PGN-induced activation of JNK and NF-kappaB signaling pathway.
! m9 d. u7 u9 _) B, r/ Z. X" R Keywords: Drosophila TAB2: C9 i; b: S6 |
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