求助 谁有SRY基因组序列

yangpan521

  最近想做小鼠的雌雄鉴定 ，但没有SRY基因组序列 ，有时新手怕引物设计不好，所以求助谁有相关的的文献请赐教

goodboy

小鼠的雌雄鉴定？直接观察不就可以了吗？为什么要做SRY基因鉴定呢？不是很麻烦吗

yangpan521

有用的  回头告诉你

279042256

回复 yangpan521 的帖子+ X9 b. L1 ?& A3 Y

! s" C1 ?/ O& r* r; I7 h% T8 {Mus musculus sex determining region of Chr Y (Sry), mRNA/ p- N: T5 l0 l" ?3 G
ORIGIN      9 J* B  P! |2 `, l5 \
        1 atggagggcc atgtcaagcg ccccatgaat gcatttatgg tgtggtcccg tggtgagagg
" O: ~, b1 f- g- \       61 cacaagttgg cccagcagaa tcccagcatg caaaatacag agatcagcaa gcagctggga
. i) {+ T4 Q% N9 w5 e7 m      121 tgcaggtgga aaagccttac agaagccgaa aaaaggccct ttttccagga ggcacagaga
3 A% n# d% L2 |" o0 {; C. H      181 ttgaagatcc tacacagaga gaaataccca aactataaat atcagcctca tcggagggct+ \0 f4 A- `& z! c6 ]) k6 t
      241 aaagtgtcac agaggagtgg cattttacag cctgcagttg cctcaacaaa actgtacaac8 q3 `, w+ S" t7 F" B- v# F
      301 cttctgcagt gggacaggaa cccacatgcc atcacataca ggcaagactg gagtagagct% z, c' A, J1 ]7 p8 X3 Y" J5 P, s. N
      361 gcacacctgt actccaaaaa ccagcaaagc ttttattggc agcctgttga tatccccact5 }$ ^- e7 B1 i" i5 [4 W: s
      421 gggcacctgc agcagcagca gcagcagcag cagcagcagc agttccataa ccaccaccag
7 H+ @& Q# A/ }/ y0 S  I+ j; h5 u      481 cagcaacagc agttctatga ccaccaccag cagcagcagc agcagcagca gcagcagcag; h5 ~+ @+ E* i$ j1 |  R
      541 cagttccatg accaccacca gcagaagcag cagtttcatg accaccacca gcagcaacag
6 ^; |5 C2 S4 d/ L! ?1 t      601 cagttccatg accaccacca ccaccaccag gagcagcagt tccatgacca ccaccagcag2 ~. P. t! F: Q5 G1 R  ]# @1 x3 k- p
      661 caacagcagt tccatgacca ccagcagcag cagcagcagc agcagcagca gcagttccat
* x) B% ~5 q3 H7 ]" J' ?! i      721 gaccaccacc agcagaagca gcagttccat gaccaccacc accaccaaca gcagcagcag
3 `9 c3 Y( P9 t" B      781 ttccatgacc accagcagca gcagcagcag ttccatgacc accagcagca gcagcatcag
; ?% o, z9 f1 K; ]      841 ttccatgacc acccccagca gaagcagcag ttccatgacc acccccagca gcaacagcag
8 e2 ]/ w, }. e6 @$ m& J      901 ttccatgacc accaccacca gcagcagcag aagcagcagt tccatgacca ccaccagcag" L- G2 X5 n  j
      961 aagcagcagt tccatgacca ccaccagcag aagcagcagt tccatgacca ccaccagcag1 Y4 n0 O4 ^! Q; g, k
     1021 caacagcagt tccatgacca ccaccagcag cagcagcagc agcagcagca gcagcagcag
5 B/ a4 h! K/ l; ^1 O# [+ X     1081 cagttccacg accagcagct tacctactta ctaacagctg acatcactgg tgagcataca
2 X2 Y- w, }; U, V' h" Y* c; N     1141 ccataccagg agcacctcag cacagccctg tggttggcag tctcatga7 B" N* K# p. e- |6 k/ S
//

279042256

回复 yangpan521 的帖子- C* @& H! }: y( W9 ~% \9 u! d+ |/ u

5 C6 c: ^& d1 Jsex-determining region Y protein [Mus musculus]
8 y! w' l& S  PORIGIN      $ R& P0 D9 d  M7 L" K
        1 meghvkrpmn afmvwsrger hklaqqnpsm qnteiskqlg crwkslteae krpffqeaqr
: N; U; v' t" c7 S* z/ r       61 lkilhrekyp nykyqphrra kvsqrsgilq pavastklyn llqwdrnpha ityrqdwsra
. V3 R* C8 ^- }" i/ ~      121 ahlysknqqs fywqpvdipt ghlqqqqqqq qqqqfhnhhq qqqqfydhhq qqqqqqqqqq
4 g7 ~: R0 M4 q. Z- M+ i      181 qfhdhhqqkq qfhdhhqqqq qfhdhhhhhq eqqfhdhhqq qqqfhdhqqq qqqqqqqqfh* n, Y2 c- h$ ?1 Z/ s7 |
      241 dhhqqkqqfh dhhhhqqqqq fhdhqqqqqq fhdhqqqqhq fhdhpqqkqq fhdhpqqqqq. I9 V# i! E5 K) w/ s' f$ C
      301 fhdhhhqqqq kqqfhdhhqq kqqfhdhhqq kqqfhdhhqq qqqfhdhhqq qqqqqqqqqq
2 J2 l2 a% I5 M+ o      361 qfhdqqltyl ltaditgeht pyqehlstal wlavs3 ]9 M$ K" G; Q2 r) b

279042256

回复 yangpan521 的帖子
4 J: D- h- f' Y
7 N: S: l( y" t
* v+ ~9 `3 C$ S. r6 kMus musculus strain C57BL/6J chromosome Y, MGSCv37 C57BL/6J
$ d. r  J% r. E2 f6 M1 G
, w4 X, r5 B! S' gNCBI Reference Sequence: NC_000087.6$ P, H3 ^9 k6 I- M2 b( Y( i# Q. d/ W

2 \1 _4 j( I9 SGenBankGraphics. ^4 b  s0 g4 A4 y# l

& `- N# S- Y, O
3 m# z3 z) }# [ % F4 G6 i1 V; z( }5 V$ b3 X
4 b" j5 T5 Q, T' ~
>gi|149361526:c1919568-1918381 Mus musculus strain C57BL/6J chromosome Y, MGSCv37 C57BL/6J# @9 e. b/ |8 |  v: |1 z8 Q
ATGGAGGGCCATGTCAAGCGCCCCATGAATGCATTTATGGTGTGGTCCCGTGGTGAGAGGCACAAGTTGG
+ U# ]8 f- D: E1 ~CCCAGCAGAATCCCAGCATGCAAAATACAGAGATCAGCAAGCAGCTGGGATGCAGGTGGAAAAGCCTTAC
4 r% d; g5 K8 v6 }AGAAGCCGAAAAAAGGCCCTTTTTCCAGGAGGCACAGAGATTGAAGATCCTACACAGAGAGAAATACCCA3 O% \, S6 Y: k4 O( a) w
AACTATAAATATCAGCCTCATCGGAGGGCTAAAGTGTCACAGAGGAGTGGCATTTTACAGCCTGCAGTTG
4 t  ?% O6 @: Q/ E: _$ f+ bCCTCAACAAAACTGTACAACCTTCTGCAGTGGGACAGGAACCCACATGCCATCACATACAGGCAAGACTG
! Z2 a/ K& J9 r' gGAGTAGAGCTGCACACCTGTACTCCAAAAACCAGCAAAGCTTTTATTGGCAGCCTGTTGATATCCCCACT
: ~0 n6 D2 i. Y3 X0 p5 H% fGGGCACCTGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCATAACCACCACCAGCAGCAACAGC0 ]) `8 s1 O% u- m4 z- p8 g$ e
AGTTCTATGACCACCACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCATGACCACCACCA
2 ~7 Z2 j' M( M6 E- g# `) r' wGCAGAAGCAGCAGTTTCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCACCACCACCACCAG% I6 z( |  s0 ?9 i& w
GAGCAGCAGTTCCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCAGCAGCAGCAGCAGCAGC& `/ v8 [' f2 w8 C3 |
AGCAGCAGCAGCAGTTCCATGACCACCACCAGCAGAAGCAGCAGTTCCATGACCACCACCACCACCAACA
( O5 X8 D' j2 z3 x) DGCAGCAGCAGTTCCATGACCACCAGCAGCAGCAGCAGCAGTTCCATGACCACCAGCAGCAGCAGCATCAG
$ g% F4 q2 v0 O0 uTTCCATGACCACCCCCAGCAGAAGCAGCAGTTCCATGACCACCCCCAGCAGCAACAGCAGTTCCATGACC; P6 t# }: S8 k# s% v
ACCACCACCAGCAGCAGCAGAAGCAGCAGTTCCATGACCACCACCAGCAGAAGCAGCAGTTCCATGACCA
/ z( y1 H: b* `, i) `6 pCCACCAGCAGAAGCAGCAGTTCCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCACCAGCAG3 F: u: `- [' J# v( d0 x# V
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCACGACCAGCAGCTTACCTACTTACTAACAGCTG. [/ H% \7 s" b0 n* v' v
ACATCACTGGTGAGCATACACCATACCAGGAGCACCTCAGCACAGCCCTGTGGTTGGCAGTCTCATGA
) u% U6 k$ _. T& B* v0 {6 Q; k% m
# E) {/ _2 X! }+ \; J

279042256

小鼠性别鉴定技术很成熟，找点Y特异基因设计个引物跑个PCR就行，很简单，但是要注意防止假阳性和假阴性，两个对照一定要做好

yangpan521

谢谢各位

yangpan521

回复 279042256 的帖子7 g) }, D# \" j: A4 Q# b

4 }( t# E' ^* H3 X7 w" h我是要鉴定囊胚 ，请问如果囊胚数量少到1,2个时还能鉴定吗？

279042256

是1-2个囊胚还是1-2个细胞啊，小鼠上没做过，牛上可以，切割下几个细胞就可以做鉴定的