求助 谁有SRY基因组序列

yangpan521

  最近想做小鼠的雌雄鉴定 ，但没有SRY基因组序列 ，有时新手怕引物设计不好，所以求助谁有相关的的文献请赐教

goodboy

小鼠的雌雄鉴定？直接观察不就可以了吗？为什么要做SRY基因鉴定呢？不是很麻烦吗

yangpan521

有用的  回头告诉你

279042256

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/ a9 B1 [% E5 m0 d: z9 r& O* ~Mus musculus sex determining region of Chr Y (Sry), mRNA1 g* ^, b' \- _9 `& \& _+ f
ORIGIN      3 W! a9 A1 J, f0 X
        1 atggagggcc atgtcaagcg ccccatgaat gcatttatgg tgtggtcccg tggtgagagg
  U! K% ~9 C9 g( D: c* Y) Z7 s; U       61 cacaagttgg cccagcagaa tcccagcatg caaaatacag agatcagcaa gcagctggga8 g) d9 y( U7 `) `5 ^2 F' o! L
      121 tgcaggtgga aaagccttac agaagccgaa aaaaggccct ttttccagga ggcacagaga0 X  r$ P6 I/ U5 v
      181 ttgaagatcc tacacagaga gaaataccca aactataaat atcagcctca tcggagggct/ L: I( ^) {. Z' |3 ]1 i2 d# r) D
      241 aaagtgtcac agaggagtgg cattttacag cctgcagttg cctcaacaaa actgtacaac
' k- o1 [% ]. j) i# ]# r      301 cttctgcagt gggacaggaa cccacatgcc atcacataca ggcaagactg gagtagagct( H+ O; |* ?) O/ k! {1 C: n1 m
      361 gcacacctgt actccaaaaa ccagcaaagc ttttattggc agcctgttga tatccccact
5 F  Z& o' M0 c, W5 R3 R  C1 x      421 gggcacctgc agcagcagca gcagcagcag cagcagcagc agttccataa ccaccaccag
! n2 F4 D1 _( P+ i9 `+ R: e# h' g      481 cagcaacagc agttctatga ccaccaccag cagcagcagc agcagcagca gcagcagcag
- M4 O1 _1 w, _4 s' N      541 cagttccatg accaccacca gcagaagcag cagtttcatg accaccacca gcagcaacag: @, d7 P  N/ K: g
      601 cagttccatg accaccacca ccaccaccag gagcagcagt tccatgacca ccaccagcag/ w! b: t! I0 U
      661 caacagcagt tccatgacca ccagcagcag cagcagcagc agcagcagca gcagttccat
8 h% B  Y) w' y& o8 f      721 gaccaccacc agcagaagca gcagttccat gaccaccacc accaccaaca gcagcagcag6 Q2 W6 C) G* b8 P8 K# u
      781 ttccatgacc accagcagca gcagcagcag ttccatgacc accagcagca gcagcatcag
( q1 @' {' I5 E4 J' ~/ ?- s. k      841 ttccatgacc acccccagca gaagcagcag ttccatgacc acccccagca gcaacagcag
- U- y. G6 M" ?. _6 ^* ~. V1 a  ], L      901 ttccatgacc accaccacca gcagcagcag aagcagcagt tccatgacca ccaccagcag6 k, o% K5 h8 R  {
      961 aagcagcagt tccatgacca ccaccagcag aagcagcagt tccatgacca ccaccagcag% r0 ?  P4 e: U8 j2 a
     1021 caacagcagt tccatgacca ccaccagcag cagcagcagc agcagcagca gcagcagcag: ?# v: _7 p/ o" f1 l
     1081 cagttccacg accagcagct tacctactta ctaacagctg acatcactgg tgagcataca
8 q8 m/ M' d. i: A& [8 x     1141 ccataccagg agcacctcag cacagccctg tggttggcag tctcatga% ~6 P1 \  Y0 F( ^8 Q
//

279042256

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7 U8 A, B) Z, \6 y
sex-determining region Y protein [Mus musculus]7 L. T/ e- W/ f; N4 {$ L
ORIGIN      
7 u; i: N2 u$ }+ |        1 meghvkrpmn afmvwsrger hklaqqnpsm qnteiskqlg crwkslteae krpffqeaqr
4 N$ s9 M( E8 ]       61 lkilhrekyp nykyqphrra kvsqrsgilq pavastklyn llqwdrnpha ityrqdwsra) U9 c; U+ m5 \( V7 V  y
      121 ahlysknqqs fywqpvdipt ghlqqqqqqq qqqqfhnhhq qqqqfydhhq qqqqqqqqqq
, X, Q0 U- a  F* |7 i) D2 `      181 qfhdhhqqkq qfhdhhqqqq qfhdhhhhhq eqqfhdhhqq qqqfhdhqqq qqqqqqqqfh
5 ~7 B. h" Q* l- W1 U* _! T      241 dhhqqkqqfh dhhhhqqqqq fhdhqqqqqq fhdhqqqqhq fhdhpqqkqq fhdhpqqqqq; s6 ^: n( j. {% d. Z
      301 fhdhhhqqqq kqqfhdhhqq kqqfhdhhqq kqqfhdhhqq qqqfhdhhqq qqqqqqqqqq  L& u1 b2 w% o; b
      361 qfhdqqltyl ltaditgeht pyqehlstal wlavs9 J; z) c- Q9 p5 |

279042256

回复 yangpan521 的帖子: ?4 t* }* k9 l1 R7 K6 k

  I; P% V! @1 y6 s) {0 E! [
. L( ^1 M, F2 cMus musculus strain C57BL/6J chromosome Y, MGSCv37 C57BL/6J' p' ^' u8 X2 {
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NCBI Reference Sequence: NC_000087.64 N, Z5 ^: [% o4 I

: _: f5 x/ b) U0 WGenBankGraphics
6 t" P6 v6 @8 e2 U6 C$ \% n: B5 @$ x  c; j$ G: r

  Y! o+ T7 B: `( U+ D* W: L
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/ d4 r+ G1 J* V5 ]! j>gi|149361526:c1919568-1918381 Mus musculus strain C57BL/6J chromosome Y, MGSCv37 C57BL/6J3 y; ^2 Z- @  G
ATGGAGGGCCATGTCAAGCGCCCCATGAATGCATTTATGGTGTGGTCCCGTGGTGAGAGGCACAAGTTGG3 M, z0 {1 q* v, w& ?  ]
CCCAGCAGAATCCCAGCATGCAAAATACAGAGATCAGCAAGCAGCTGGGATGCAGGTGGAAAAGCCTTAC
0 V$ L5 C1 x, ]& p0 P& bAGAAGCCGAAAAAAGGCCCTTTTTCCAGGAGGCACAGAGATTGAAGATCCTACACAGAGAGAAATACCCA
, t' z7 S$ E- ~/ }AACTATAAATATCAGCCTCATCGGAGGGCTAAAGTGTCACAGAGGAGTGGCATTTTACAGCCTGCAGTTG
6 O' \/ C6 k* [% nCCTCAACAAAACTGTACAACCTTCTGCAGTGGGACAGGAACCCACATGCCATCACATACAGGCAAGACTG
2 c' Y" N+ Q2 ?1 h( S1 n0 ~, M7 H9 ?GAGTAGAGCTGCACACCTGTACTCCAAAAACCAGCAAAGCTTTTATTGGCAGCCTGTTGATATCCCCACT" S+ y% u! P4 f( \' J
GGGCACCTGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCATAACCACCACCAGCAGCAACAGC
) S' `. K, V) j' t7 pAGTTCTATGACCACCACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCATGACCACCACCA
, p  z* H' f6 v2 _4 iGCAGAAGCAGCAGTTTCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCACCACCACCACCAG
* ]. n7 ~9 `+ t7 E1 k% cGAGCAGCAGTTCCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCAGCAGCAGCAGCAGCAGC' Y3 p: N5 L. T: e! u9 a
AGCAGCAGCAGCAGTTCCATGACCACCACCAGCAGAAGCAGCAGTTCCATGACCACCACCACCACCAACA
6 T1 p: h. A8 s$ e" \6 j  t( GGCAGCAGCAGTTCCATGACCACCAGCAGCAGCAGCAGCAGTTCCATGACCACCAGCAGCAGCAGCATCAG" d* n, F8 V, o, U3 C
TTCCATGACCACCCCCAGCAGAAGCAGCAGTTCCATGACCACCCCCAGCAGCAACAGCAGTTCCATGACC: W# T, e! L! f9 B; k
ACCACCACCAGCAGCAGCAGAAGCAGCAGTTCCATGACCACCACCAGCAGAAGCAGCAGTTCCATGACCA
& W* a3 L1 X! }7 R8 OCCACCAGCAGAAGCAGCAGTTCCATGACCACCACCAGCAGCAACAGCAGTTCCATGACCACCACCAGCAG
1 J) \# _; J  U) OCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTTCCACGACCAGCAGCTTACCTACTTACTAACAGCTG" Y0 G5 \7 C: t( \" p
ACATCACTGGTGAGCATACACCATACCAGGAGCACCTCAGCACAGCCCTGTGGTTGGCAGTCTCATGA/ g$ h' `% {; F9 u, p3 M, B) |

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279042256

小鼠性别鉴定技术很成熟，找点Y特异基因设计个引物跑个PCR就行，很简单，但是要注意防止假阳性和假阴性，两个对照一定要做好

yangpan521

谢谢各位

yangpan521

回复 279042256 的帖子  X8 W( v7 E, b8 F9 Z5 t

6 |/ {8 C5 w# h6 ~0 _/ O8 k% g6 Y我是要鉴定囊胚 ，请问如果囊胚数量少到1,2个时还能鉴定吗？

279042256

是1-2个囊胚还是1-2个细胞啊，小鼠上没做过，牛上可以，切割下几个细胞就可以做鉴定的