PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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Springer MMB Vol931 2013 共29章 552pages
7 |: P/ r# Z1 t; W: H6 j$ v: {Cell Imaging Techniques  Methods and Protocols
' k! @- g& y% ~# @2 ESecond Edition! S  ]4 U8 v3 A
Edited by
: c, u6 e7 T' HDouglas J. Taatjes
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Contents6 `# U4 l% K) s1 h- r/ g6 l5 z

- H+ P2 ?2 e5 U' d/ R* m  n: }8 SPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii
9 ?1 R# @# c' G8 @: zContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii
4 T! y8 P* h7 T  P; l1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       1
0 o2 ]4 [+ @  Y( r7 uDouglas W. Cromey, K- T6 A0 B- S- _( `' l' i. A
2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29) [- F; C: @7 X+ b
Donna J. Webb and Claire M. Brown
) @1 ~8 {2 p$ s  g, d3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   613 }' C# k) _1 S( m! a. n
J. Lacoste, K. Young, and Claire M. Brown) U0 {' n/ n9 T- p& f
4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
% s" ^" i2 F4 u" q6 J9 yProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 855 @7 P5 ]8 i! |' Y, C3 z
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
6 X" A) Y; _( E# {5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97$ W' o, D9 S" W, f  d
Jeremy Adler and Ingela Parmryd
6 Z, ]" E5 ~9 \& [4 k6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    1112 s: l1 l3 B9 a+ V3 |& |
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman, p8 ]& Y" {1 x2 S+ K2 [0 l
7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
: n- G# ^  B& K! b8 z8 jRaju Tomer, Khaled Khairy, and Philipp J. Keller5 Q+ }* A( N$ [. N
8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139- l: `1 Z/ q1 C5 `3 d' Q4 `
Lasse Evensen, Wolfgang Link, and James B. Lorens
8 Z9 ^& `" m% a9 m9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     1535 @5 i9 s8 q) @7 Z/ b& c0 w
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert7 _6 x+ O- @+ w! s/ G  q3 M
10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
0 a& J8 Q% ?" V" a6 aInterference
6 j$ U5 f. z( n4 P5 {) F" J: XContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169# S8 Y: v. V( _6 l! R& m3 y0 e2 y/ ^
Wei Sun, Lehui Xiao, and Ning Fang% I  ^' P. u* s/ c1 U
11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187/ f) J7 g2 Q7 n6 p1 u0 Y
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
* z* U$ x3 K2 U4 m( a) m12   Laser Capture Microdissection for Protein  z( I7 g: x- l6 r6 r0 g
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
3 j" r) p, h& c8 M( GYelena Golubeva, Rosalba Salcedo, Claudius Mueller,% q9 L9 D2 m5 t1 r9 @
Lance A. Liotta, and Virginia Espina  J3 M/ {0 k; W6 Z7 |
13   Viewing Dynamic Interactions of Proteins and a Model Lipid2 D/ K8 K4 O* \
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259
! k' N* X. R  r9 N& ^( ~0 \Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes2 k7 u6 Y4 D3 c8 ~" R( t$ s' M
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
( m% |& w) ?0 vLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
0 `( n9 i& \7 r$ G9 T15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313' F  o+ Q9 B; f7 l. `* @
Joanna L. MacKay and Sanjay Kumar
+ D/ I2 n( e' h! \9 Q4 W* T16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
8 t/ a0 D4 h: \/ E( k' E. V* ELilia A. Chtcheglova and Peter Hinterdorfer
" j& ?" X" N, L17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     3450 r+ u- |* \" v3 T
Bhanu P. Jena: O+ ~& U$ _9 S/ J
18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367: z) k5 X% [- b: y3 K7 o4 ~
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs  X8 V5 e; E- r( B* ^# o
19 A Novel Combined Imaging/Morphometrical Method for the Analysis
+ i. @+ F5 v. W% P! gof Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    391
  s1 O$ b* i$ S8 p" G6 Z2 sMichele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
; g$ A" N. v; k' r0 D' `* p20   Correlative Light–Electron Microscopy as a Tool to Study6 O0 ~( M$ D: Q; D# `
In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413- x2 b4 W+ W6 j
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
, T2 ^$ B$ z1 U* U1 K7 v) ~/ }21   Photooxidation Technology for Correlative Light( p( K* F! I3 w
and Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423, _+ g" m3 }4 O7 j- y6 @
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller,
7 c5 d: h7 _0 M1 TMargit Pavelka, and Adolf Ellinger
7 W$ h) ?! x) y! C& b9 G22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437
9 T5 X0 U5 H# _" E$ G0 T3 PCarmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and
; e& X$ g4 O& pMargit Pavelka
$ ^: R. Q4 z+ g5 }9 `23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     449; ?9 u4 R4 U( j" I5 ]% M
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori
( C: s8 \+ `9 O0 ^24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
0 a- ]% ]& M5 F: BNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader# c, X7 l# T7 ]( O) N
25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     4833 ]) y$ M, \3 M: q) Y
Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
$ k, N  B6 ^7 v9 R! l  u26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    4939 A, B1 Y* P4 H4 J6 w" L
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald2 |8 X  k' l% @5 h" m
27   Environmental Scanning Electron Microscopy Gold6 K2 b9 I2 M- v* t
Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517
) G" b9 z' \2 d7 T7 }Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi% c# \# Z5 D! b/ X7 u+ H( a
28   High-Pressure Freezing for Scanning Transmission Electron
2 k' W& I8 N6 H6 x: iTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     5252 o1 z" h* k1 @- @7 u7 t4 v! N
Paul Walther, Eberhard Schmid, and Katharina Höhn' u1 H/ t5 s* k  G
29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     537* T' u& m" ?$ D! C
Stephan Meding and Axel Walch
1 x$ |9 {* G+ }* BIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547
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