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本帖最后由 细胞海洋 于 2013-1-8 09:22 编辑 - Y: |5 ~) {# S! B8 \
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- t# T' X6 u; I. v, ISpringer MMB Vol931 2013 共29章 552pages
0 `9 {. }/ p3 J, L w8 HCell Imaging Techniques Methods and Protocols
; c0 z' R( e1 g1 A* RSecond Edition0 n8 o* H8 U0 m; j. I" J
Edited by( G, y& I: \- @* U6 I
Douglas J. Taatjes
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# v l+ U7 H/ ^$ HContents
" k- u3 B2 \6 G5 \0 s7 i, d. @! B1 I! X$ c7 P& C! K$ O' ^
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
^& \2 x! K _. J4 `* `( NContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
& q2 V5 y4 H7 Q/ g1 Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . . 1* W4 E% L' C% p5 y
Douglas W. Cromey, e0 S, z5 [; y4 C( m: u
2 Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
: `" {" |: q# i7 v) aDonna J. Webb and Claire M. Brown, T H/ L* p5 Z4 j) g1 k
3 Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . . . . . . . . . . . . . 61: |4 s+ G+ N* W5 D0 Z) ?* b; b
J. Lacoste, K. Young, and Claire M. Brown* n! m; @/ w) L$ I
4 Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
7 e6 [$ h3 H7 j/ C9 ]; I1 U: t: MProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 856 l! V! P; s* S O L# F' M
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
4 L+ p& E8 d* u8 E5 Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . 97
' F$ h3 W' `4 N( E. S/ GJeremy Adler and Ingela Parmryd& X2 X5 T, l7 B5 x' r, d R; F+ c
6 A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . . 111
- x0 R: a: x* z4 n jSunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
: }0 Q" [' V8 K h& @% \2 J7 Light Sheet Microscopy in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123# B" D ^% M5 o8 [
Raju Tomer, Khaled Khairy, and Philipp J. Keller
. |8 |! ~, u9 |/ E+ M. d3 Y8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
- x/ s [- j& f" F5 YLasse Evensen, Wolfgang Link, and James B. Lorens# q" r9 u. f. o
9 Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . . 153; S& p) B j; B8 Y4 d
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert, U" Z9 o3 w0 L
10 Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential 5 }3 l( @" m/ t+ h
Interference
9 {0 Z+ W' E' l1 ~* [0 KContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . . 169
4 f! Z8 e$ a1 v0 [& k3 L5 s+ m) KWei Sun, Lehui Xiao, and Ning Fang
$ k. }( D3 Z$ Q" \5 {- Q0 Z3 |11 Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . . 1874 S! o6 j; E: j7 v
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
1 n6 V, y, @* U2 @% T9 h12 Laser Capture Microdissection for Protein+ c. @$ d# r2 E8 p% d
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2136 d! {1 J" R- S4 e; h/ @
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,8 z/ N8 j# i8 \/ r# ^1 x9 Z
Lance A. Liotta, and Virginia Espina
. \# Q; _% o; K, \( t: D& e" H13 Viewing Dynamic Interactions of Proteins and a Model Lipid
& \ _% `/ L. C$ o8 O8 yMembrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . 259: d/ R& c# \3 r9 |0 K8 W
Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes
: E! ?) I) w# h3 a& \- K14 Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
# w$ E2 B/ B- h, E) }8 DLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
& a+ q0 H L9 X! X# Z15 Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313- G( m1 G8 n2 t; r5 G0 D1 I
Joanna L. MacKay and Sanjay Kumar/ E" ]' z* j' B# _
16 Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3315 s3 _5 w8 p, K5 Z
Lilia A. Chtcheglova and Peter Hinterdorfer
0 f+ A& M6 @- Q* v4 f% g; h7 o5 N17 Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . . 345& W) n7 I' d9 u+ k" w; R2 ~
Bhanu P. Jena
' a# i* [8 ^ g( N* Z1 Q1 t18 Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . . 3678 D5 ?4 M, ^- J, H
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs4 ?) D7 L. G' ^; v$ ~; n6 \# D
19 A Novel Combined Imaging/Morphometrical Method for the Analysis9 H* C5 c9 ^; }
of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . . 391
3 H9 K0 D" d$ W4 a) U) vMichele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
1 j% ? `8 o8 @) s: f20 Correlative Light–Electron Microscopy as a Tool to Study
8 j8 e( _0 ~3 F, J- }1 {) nIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . . 413
$ E5 y: Q! ^5 t4 [( IElena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini. ]% }( a/ B1 D' D4 l
21 Photooxidation Technology for Correlative Light
; Q Y3 h' S% E- Q4 L/ l- Z3 a) m: Oand Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 423
+ p, C3 p. p! gClaudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller,
! u c+ C% Z: X: S, HMargit Pavelka, and Adolf Ellinger4 {, S' M9 G( c& r. M
22 Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . . 437
# |5 x$ S9 O5 |- eCarmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and 3 `* L' z, N! \
Margit Pavelka8 c, U5 l {" K
23 Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4496 g% C0 S: ]; B# W
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori
' R( P! {' p" [4 y o# \24 Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . . 467
# \* A! {9 x2 ^. ]Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader1 y0 v5 E8 H6 J8 L7 a* R" p, v
25 Histochemical Detection of Lipid Droplets in Cultured Cells . . . . . . . . . . . . . 483
8 R2 D& \2 C! ?2 x$ O- R yMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto, r M6 X& q4 }' i: x
26 Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . . 493
6 @" M* ]0 P# U9 I fJ.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald4 L- x3 J' H: Q8 T, k' I
27 Environmental Scanning Electron Microscopy Gold
6 W) t! A: n8 o! `3 t4 }Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 517
, E; M% O1 U6 z2 hFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi1 k! @ \$ [( O7 x" J8 V% }
28 High-Pressure Freezing for Scanning Transmission Electron- |; m8 U) D7 p# m
Tomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . . 525; E! F n, e( T
Paul Walther, Eberhard Schmid, and Katharina Höhn7 W; q5 K! D3 a# M9 b% L: E. Y
29 MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . . 537( q5 F: N+ e3 s: ]7 t+ H4 ]2 [
Stephan Meding and Axel Walch
6 L" \6 O8 ]) E; S- v- AIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547
3 C* b; |/ U1 \1 u! U4 f" \. u2 h, Z' P, f! l* }+ x
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