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Rapid Attachment of Adipose Stromal Cells on Resorbable Polymeric
% q4 v" K7 a4 E1 e0 w" I6 EScaffolds Facilitates the One-Step Surgical Procedure for Cartilage and% |: b' N( I; E/ L: Q
Bone Tissue Engineering Purposes# A9 @3 w, _( E! d3 N. J0 Z; F
Wouter J. Jurgens,1,2 Robert Jan Kroeze,2,3,4 Ruud A. Bank,2,5 Marco J. P. F. Ritt,1,2 Marco N. Helder2,3 V6 B+ j s+ ^& F2 }7 I' }% \+ S8 N; A& \" I
1Department of Plastic, Reconstructive and Hand Surgery, VU University Medical Center, Amsterdam, The Netherlands, 2MOVE/Skeletal Tissue
' v) n! N J+ g( M) hEngineering Group Amsterdam (STEGA), Amsterdam, The Netherlands, 3Department of Orthopaedic Surgery, VU University Medical Center,4 t8 _: C$ g; R! r6 o1 |, e% i4 g
Amsterdam, The Netherlands, 4Department of Oral Cell Biology Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam4 _$ f, T$ T' J4 K7 @
and Vrije Universiteit, Amsterdam, The Netherlands, 5Department of Medical Biology, University Medical Center Groningen, Groningen, The7 B" K! A- {: U- S, N
Netherlands4 G' M, D7 \2 j7 ^' n) z
Received 3 May 2010; accepted 8 October 2010
4 w& R6 Q9 F& H; U! g+ V1 d& a3 jPublished online 18 January 2011 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/jor.21314. S2 I, M/ L, m2 F- u2 K; J0 t& T
ABSTRACT: The stromal vascular fraction (SVF) of adipose tissue provides an abundant source of mesenchymal stem cells. For clinical) ]1 o% T2 J6 {- P3 X" K) c
application, it would be beneficial to establish treatments in which SVF is obtained, seeded onto a scaffold, and returned into the patient/ O+ y0 {' H) p3 a6 w6 n( o
within a single surgical procedure. In this study, we evaluated the suitability of both a macroporous poly(L-lactide-co-caprolactone) and a
: O$ F% k' f# ]* J/ |) L1 A* Yporous collagen type I/III scaffold for this purpose. Surprisingly, cell attachment wasrapid (10 min) and sequestered the majority of adipose% v0 J% w: Z2 H. ~
stem cells, as deduced from colony-forming unit assays. Proliferation occurred in both polymeric scaffolds. Upon chondrogenic induction, upregulation0 b" ~9 a& c- T& d5 W8 j$ i, `
of chondrogenic genes, production of glycosaminoglycans, and accumulation of collagen type II was observed, indicating- n7 j( W5 b& V! W7 c* c9 @
differentiation of scaffold-attached SVF cells along the chondrogenic lineage. Osteogenic differentiation was achieved in both scaffold
& d" d: F2 P, w8 J6 |1 b/ T! n3 mtypes, as visualized by up-regulation of osteogenic genes, increase of alkaline phosphatase production over time, and accumulation of bone
" ~: P7 c5 O& w4 V6 A/ rsialoprotein and osteonectin. In conclusion, this study identifies both poly(L-lactide-co-caprolactone) and collagen type I/III as promising0 J+ s8 M0 r. g9 @. `" }
scaffold materials for rapid attachment of adipose stem cell-like (stromal) cells, enhancing the development of one-step surgical concepts& } m7 q- R' U$ c9 [7 t% D, n
for cartilage and bone tissue engineering. 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:853–
' P) U) s" {9 T" Q" B5 M860, 2011 |
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