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没有养过这种破骨细胞,但是细胞长得快就传代稀释一点。ATCC的说明是:" ~% s4 D% b9 F, C% Q; h
Subculturing: Protocol: Subcultures are prepared by scraping.
* r; i h7 R; @For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.
# h. a: h( E e- U/ F# y# uSubcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended # U* d7 t* {0 n' h- t
Medium Renewal: Replace or add medium every 2 to 3 days. 7 e; X7 M- A( R0 D! y/ }: a- N
你可以低密度传代,如果还是很快,那很有可能被其他细胞污染,不是原本的RAW,比如从别人要来的,可能被处理过,实验结果就不可靠了。这种现象国内实验室常见的,一般都买新的细胞,不要从别人索取,要不就得有MTA协议的正规获得。 |
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