- 积分
- 760
- 威望
- 760
- 包包
- 1346
|
没有养过这种破骨细胞,但是细胞长得快就传代稀释一点。ATCC的说明是:
) w, _4 F* S$ d' wSubculturing: Protocol: Subcultures are prepared by scraping.
5 t+ t9 P) @, `: Q" ]; V- K. IFor a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels. ( x3 u: q" R$ I
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
' c' T# z( r9 s6 D: w% k+ p! WMedium Renewal: Replace or add medium every 2 to 3 days.
) A5 C2 N5 ]2 y; [- X' H/ N你可以低密度传代,如果还是很快,那很有可能被其他细胞污染,不是原本的RAW,比如从别人要来的,可能被处理过,实验结果就不可靠了。这种现象国内实验室常见的,一般都买新的细胞,不要从别人索取,要不就得有MTA协议的正规获得。 |
-
总评分: 威望 + 10
包包 + 10
查看全部评分
|