PDF电子书：Basic Cell Culture Protocols (Springer MMB-Vol946, 4e, 2013)

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. g9 R  f( ^$ w, ?5 wMethods in Molecular Biology
2 {/ |4 I& `& M  B. RVolume 946 2013, {, u$ W/ x+ m9 ^& k4 Y
Basic Cell Culture Protocols
; {: x, w/ L( y" J" XEditors:% j" |6 e" p+ d" |
    Cheryl D. Helgason,( c6 w8 A5 U9 H
    Cindy L. Miller
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ISBN: 978-1-62703-127-1 (Print) 978-1-62703-128-8 (Online)' y, M4 v: Y$ J, p, k" x9 u
Table of contents (33 chapters)% F9 X% j2 E$ P# U$ ~, y
Contents6 A8 p, R4 X  \+ M2 Q
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v. H8 B$ Y& T" D1 v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi' y2 e% m# a$ i$ a1 @
1 Detection of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
/ b0 T, g, S4 G7 }2 m$ C- }' PCord C. Uphoff and Hans G. Drexler
% b% D4 W  m/ R" i! e- y$ n" ~2 Eradication of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . 157 A0 y: Q& `* s& V0 x. C4 A  q9 _
Cord C. Uphoff and Hans G. Drexler/ W2 n2 e' ?* w0 {
3 STR DNA Typing of Human Cell Lines: Detection of Intraand Interspecies Cross-Contamination and Interspecies Cross-Contamination  27
. j: X+ t  M; \; [' z: hWilhelm G. Dirks and Hans G. Drexler" F: e+ U+ \5 L+ h1 e$ ~. |
4   Classical and Molecular Cytogenetic Analysis. . . . . . . . . . . . . . . . . . . . . . . . . .393 d/ o. P, I5 L( ~6 t8 G! W& \3 a
Roderick A.F. MacLeod and Hans G. Drexler
; x1 Z. K' p8 C$ S0 ~( W5   Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle . . . . . . . . . . . . 61% b. s$ M9 v/ A8 C$ x
Linda A. Cannizzaro, o: @) R% R8 q# Y# p
6   The Development of T Lymphocytes in Fetal Thymus Organ Culture . . . . . . .       85, T: n4 E3 V& q5 ]4 S5 Z( W5 b' p
Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama
) p/ P: G: ?2 C! d7  Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors . . . . . . . . . 103
" R% S' M- J* n: h; mGénève Awong and Juan Carlos Zúñiga-Pflücker
, \6 H4 R* ~& U6 p& }5 X8   In Vitro Generation of Human T Regulatory Cells: Generation, Culture,and Analysis of FOXP3-Transduced T Cells. . . . . . .115
* h$ @4 w5 D) U( m2 Q! ~$ tAlicia N. McMurchy and Megan K. Levings7 `) k/ h' T. y) x5 D+ H) U
9   Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media. . .    133/ i5 w. w0 Q4 y0 P- N/ u
Bert Wognum and Tracy Lee
8 ~# i" t) D$ p) i+ q# h0 j' B; l' n10   Isolation and Characterization of Mouse Side Population Cells . . . . . . . . . . . .     151+ J& a+ f7 Y8 `, y5 j8 v
Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell
0 ^3 J: j  |6 i4 U8 N# i* y11  Stem Cell Identification by DyeCycle Violet Side Population Analysis . . . . . . .    163+ m$ _9 ^+ _$ s
William G. Telford2 F! j3 e/ o# Z; T
12   Isolation and Characterization of Cancer Stem Cells In Vitro  . . . . . . . . . . . . .    181
, k+ W) q  i9 |$ n$ W! N! TCraig Gedye and Laurie Ailles4 T( s* R* ^7 Q: D: i& }
13  Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets . . . . . . . 205# B, d/ F! m+ Y  p) n' j% {( C$ T5 ~
Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer: q& w) {) L+ ?$ [6 F
14   Generation and Characterization of Murine Alternatively Activated Macrophages . . . . . . . . . . . .2259 y% d8 V9 @! f3 Z0 `  ?. O8 w
Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly4 c+ t! `# r& S0 F" d, \6 }8 y( ^
15  Human Long-Term Culture Initiating Cell Assay . . . . . . . . . . . . . . . . . . . . . .    241/ R( b* O, q) _5 Q3 D1 M
Min Liu, Cindy L. Miller, and Connie J. Eaves
( I3 E. |; J6 N0 H$ f/ X16  Long-Term Culture-Initiating Cell Assay for Mouse Cells . . . . . . . . . . . . . . . .    2577 X% b) J) v% X+ K# Y4 x& C
Stefan Woehrer, Cindy L. Miller, and Connie J. Eaves
1 F  a* e" [( q" V17  Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells . . . .   267( K/ g# j0 G" Y2 J% k
Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller
) N7 G9 h0 D! J$ X2 d8 t18  Studying Leukocyte Recruitment Under Flow Conditions. . . . . . . . . . . . . . . .    285
, V2 ]  e0 J0 u) g  l; N" kSean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel+ W" ^) t0 r6 M1 j- S
19   Generation and Establishment of Murine Adherent Cell Lines. . . . . . . . . . . . .     301
2 ]2 @2 p3 @# s6 t& i4 |4 |Rouzanna Istvanffy and Robert A.J. Oostendorp
( v  C* V( n- r) w# E0 ^+ N0 D20   Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture . . . . . . 315( A4 ~5 D" d- y( W: {
Ravenska Wagey and Brenton Short0 S2 D) ?7 D/ {6 w2 N
21   Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone . . . . . . . . . . . .335
1 D/ H8 l" T9 YBrenton Short and Ravenska Wagey- o" v( q* C) L$ `8 S' }6 t
22   Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture . . . . . . . . . . .349
% q3 _* Y5 t# D+ X2 H" a6 y" y- J4 ~Katharina Schallmoser and Dirk Strunk) b$ p, ^3 w4 n8 a
23  In Vitro Methods to Culture Primary Human Breast Epithelial Cells. . . . . . . .    363
5 a1 D) z  l! |& c( }/ YAfshin Raouf and Yu Jia Sun8 r, ~+ Y+ _  ~( ^
24  Human Prostate Epithelial Cell Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3837 ~; x+ a0 X% k, C8 d: r& B- A
Johng S. Rhim* p3 M% @% d9 u
25  Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue . . . . . . . .395
: S+ T6 ], ~$ AMichael Prater, Mona Shehata, Christine J. Watson, and John Stingl( Y- k6 ?1 Z7 M/ u! x# e2 n) \
26   Isolation and Characterization of Human Hair Follicle Epithelial Cells . . . . . .     411- D, m* H' y/ j
Keita Inoue and Kotaro Yoshimura0 Z: g& L1 ]  Q, R( R
27   Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells . . . . .423
" O! z. a$ B  F( ~: b$ [& @# Z  V4 uRicarda Glaum and Margit Wiedmann-Al-Ahmad
8 Q! E9 f4 X$ c$ p5 D% p28  Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells . . . . . . 431
- _" x& W# }( |0 l3 L. M: I+ dPaul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni
: U/ J4 X: I( X9 U* _8 J/ Y3 f6 A29  Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cell ............469
8 L% |; F% x5 v/ f: S' S$ zTakamichi Ishii, Kentaro Yasuchika, and Iwao Ikai
; f5 A0 [& N9 H! d* O30   Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System . . . 479$ a" f3 y: L7 ?
Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds+ q6 z+ j2 r6 b
31  Feeder-Independent Culture Systems for Human Pluripotent Stem Cells. . . . .    507
9 c$ _- v7 I; T/ LJennifer Moody
4 O7 b( l* r0 i) b- P32   Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates . . . . . . . . 523
! \( t3 P! P+ m- M/ tJennifer Antonchuk! ^: H7 X7 `8 ]  a' S' @1 w
33   Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells. . . . . . . . . . . . . . . . . . .535
  P3 t  M2 I/ X  ANirupama K. Shevde and Amber A. Mael
6 J4 j  n8 z( S, _" b* d4 t9 [2 bIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5477 [1 @4 w5 R" ?' r6 m
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pailishi

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wujh1986

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clairezy1983

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clairezy1983

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