PDF电子书：Basic Cell Culture Protocols (Springer MMB-Vol946, 4e, 2013)

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% y- `' N0 x# |) yMethods in Molecular Biology
- T& v: z/ L) z4 A7 ~4 C. b- PVolume 946 2013
. e4 W9 d7 H4 r$ ~% YBasic Cell Culture Protocols$ ~, U, O7 L/ h- w  K
Editors:0 g7 _& t, A; n) g" \. A
    Cheryl D. Helgason,5 I5 @. @! P' z. W1 {
    Cindy L. Miller
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2 J2 o# \5 m7 c; s8 hISBN: 978-1-62703-127-1 (Print) 978-1-62703-128-8 (Online)" U% W9 {3 D- O
Table of contents (33 chapters)& T; L+ A  N' k; x/ N% A
Contents2 o' }9 g# M6 \2 _) F2 `. n
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
2 f0 m9 x0 _/ Q( w0 ~1 FContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi& |! b# r% D6 h5 Y) p9 V
1 Detection of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . . 1: r; N  p( L: I% k2 w8 L
Cord C. Uphoff and Hans G. Drexler  i% i- }0 ]" p, [) F7 S
2 Eradication of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . 15
+ m9 \; Z1 ~) u1 E9 N  ?Cord C. Uphoff and Hans G. Drexler
2 ~$ i% {# L$ i3 STR DNA Typing of Human Cell Lines: Detection of Intraand Interspecies Cross-Contamination and Interspecies Cross-Contamination  27
* P9 \7 h- L- N+ ?% z3 HWilhelm G. Dirks and Hans G. Drexler2 _+ O1 C7 V( K0 z  n$ v, F
4   Classical and Molecular Cytogenetic Analysis. . . . . . . . . . . . . . . . . . . . . . . . . .392 S) e: |: [  R' N
Roderick A.F. MacLeod and Hans G. Drexler
. {. v7 K7 q: p5   Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle . . . . . . . . . . . . 61
$ j7 }- ^3 Y' q1 ^% eLinda A. Cannizzaro
! m2 b3 P/ P7 P4 [  m8 G6   The Development of T Lymphocytes in Fetal Thymus Organ Culture . . . . . . .       85
1 E, b9 n6 H, h+ BTakeshi Nitta, Izumi Ohigashi, and Yousuke Takahama8 s9 \  F2 [" P2 I+ _' s  ?* b
7  Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors . . . . . . . . . 103
" n2 q( x; |3 ~% `$ z& ]Génève Awong and Juan Carlos Zúñiga-Pflücker5 i3 y6 d6 Y. f! H) Q/ @
8   In Vitro Generation of Human T Regulatory Cells: Generation, Culture,and Analysis of FOXP3-Transduced T Cells. . . . . . .115
! c/ @! V2 w* H( A/ dAlicia N. McMurchy and Megan K. Levings
# r: c  n! V7 M* T0 H$ Y9   Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media. . .    133" I: b. h" r% l1 M  B9 ^, o9 I8 @
Bert Wognum and Tracy Lee
9 n% Q  i. p7 U: c5 V% w* S( R10   Isolation and Characterization of Mouse Side Population Cells . . . . . . . . . . . .     151  q% l$ E# g& _( t1 Q( i
Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell
6 q; n4 j6 u0 S11  Stem Cell Identification by DyeCycle Violet Side Population Analysis . . . . . . .    163
' i( v4 W" h7 d2 b: f& S  `% a! EWilliam G. Telford
, B& S: j: u1 y0 c12   Isolation and Characterization of Cancer Stem Cells In Vitro  . . . . . . . . . . . . .    181) `, x- a9 m* t7 U9 b
Craig Gedye and Laurie Ailles
3 P6 Q+ A' X& P; b! d( l% a6 G4 A13  Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets . . . . . . . 205
# a, U' `4 Z8 u8 ?2 x3 ~Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer1 X! {. ^7 Q; E7 ~* X$ U
14   Generation and Characterization of Murine Alternatively Activated Macrophages . . . . . . . . . . . .225
! W8 [1 D) Y9 \6 r0 `2 AShelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
' H; n) f! R6 l7 L1 P15  Human Long-Term Culture Initiating Cell Assay . . . . . . . . . . . . . . . . . . . . . .    241
& o2 r0 p0 u9 `8 ^1 k* `Min Liu, Cindy L. Miller, and Connie J. Eaves  N, |0 v9 l) d
16  Long-Term Culture-Initiating Cell Assay for Mouse Cells . . . . . . . . . . . . . . . .    257/ ~4 }; B5 `/ Z! p; v/ B& d/ A" p
Stefan Woehrer, Cindy L. Miller, and Connie J. Eaves) M6 I/ t" u: a- ^- x; f$ t3 P- b6 z
17  Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells . . . .   267+ q6 m; m6 D7 V' F( |% o
Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller8 N# m/ e0 t  g0 a* r; `+ O4 j
18  Studying Leukocyte Recruitment Under Flow Conditions. . . . . . . . . . . . . . . .    285
, L( C4 A% i% ]0 }/ T& ~Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel
- A- W+ e% P9 @" X19   Generation and Establishment of Murine Adherent Cell Lines. . . . . . . . . . . . .     301% R6 r( k) d0 Y$ |# ~" R
Rouzanna Istvanffy and Robert A.J. Oostendorp5 d0 m. I+ v, `! X2 [5 O
20   Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture . . . . . . 315
' L; c. c( K' k2 E1 c! \. C6 F+ yRavenska Wagey and Brenton Short
) b# f! \4 z# a- T, K; t21   Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone . . . . . . . . . . . .335
7 E$ t1 d, d, U6 nBrenton Short and Ravenska Wagey
& K2 l9 k/ ~! T1 L; R  H: `2 _* Q22   Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture . . . . . . . . . . .349
# }) x  C/ b+ {, O2 j( H* pKatharina Schallmoser and Dirk Strunk
: Q. X! n+ J1 R) G( K6 u, H23  In Vitro Methods to Culture Primary Human Breast Epithelial Cells. . . . . . . .    363" y- d0 T! {0 h
Afshin Raouf and Yu Jia Sun6 p8 y/ n% k: @' b, H2 _( [0 N% \
24  Human Prostate Epithelial Cell Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3834 h. l9 X) }. W( N" c6 @
Johng S. Rhim
, Y. p- o+ I8 t/ \3 \+ C* m7 q9 \( B25  Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue . . . . . . . .395
' T- M4 |+ ^& O9 f  ~Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl, U+ \. R3 Y: x, L/ V$ J2 k
26   Isolation and Characterization of Human Hair Follicle Epithelial Cells . . . . . .     411
- |; d6 C7 Z; HKeita Inoue and Kotaro Yoshimura
, P  C5 d. N, |( n4 Y2 G; w: q27   Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells . . . . .4231 f$ s8 Z( a: v, {
Ricarda Glaum and Margit Wiedmann-Al-Ahmad- K6 y$ j6 R9 x& }: B- }
28  Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells . . . . . . 431
/ J  T/ H+ B' w/ d9 G( A' e& H2 VPaul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni
+ j6 a- K4 s8 P  H( ?( N29  Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cell ............469. F% N6 G0 }  |0 h  O
Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai$ }; ?7 }$ ^" p% z+ u1 e  k" ]8 @
30   Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System . . . 479+ z4 x/ T( K4 {
Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds: m  K; _$ c" W- @: N8 [
31  Feeder-Independent Culture Systems for Human Pluripotent Stem Cells. . . . .    507
0 ^; U- G4 x1 M% I, y) U$ R9 `Jennifer Moody% {, x/ i: x; K8 X$ R+ ?$ U. H6 ]
32   Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates . . . . . . . . 523
% G6 O0 F6 R+ y* d1 \( c  IJennifer Antonchuk) \& l( V& Z8 K4 D8 j
33   Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells. . . . . . . . . . . . . . . . . . .535' F1 Y5 V5 f5 w2 k# [( Y1 q6 D0 e
Nirupama K. Shevde and Amber A. Mael
6 v6 _% c: U0 Q* W+ sIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547# [* @8 p$ b1 i, L2 }! N
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