PDF电子书：Basic Cell Culture Protocols (Springer MMB-Vol946, 4e, 2013)

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Methods in Molecular Biology
4 @- a; i6 C0 K$ P* j; }; tVolume 946 2013# A1 ^5 n; v! V5 T* \
Basic Cell Culture Protocols- U1 N9 l  L( p/ @" n8 W7 l
Editors:
9 j+ z6 {. [) b. _    Cheryl D. Helgason,) ~! M/ K8 J& b5 @
    Cindy L. Miller
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ISBN: 978-1-62703-127-1 (Print) 978-1-62703-128-8 (Online)
. U8 S0 Z, I5 J6 a) J! `9 N, U3 PTable of contents (33 chapters)
5 ^( k6 G# U6 Y: X! _, G, KContents
4 n  \4 k2 l5 c$ r% Q% t7 Z% fPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v' C; F. {; u3 r% N' I( f. p( j7 ]
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
8 A0 |: T1 s4 [  A8 u: M1 Detection of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
$ R: _* U( L6 OCord C. Uphoff and Hans G. Drexler
6 S6 i% v; v/ k2 g; `/ v: P! [2 Eradication of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . 15
6 I4 N4 p. f! `. @" kCord C. Uphoff and Hans G. Drexler3 R8 ]6 }; f9 I. S: F* q
3 STR DNA Typing of Human Cell Lines: Detection of Intraand Interspecies Cross-Contamination and Interspecies Cross-Contamination  272 Q4 U7 _6 f3 V) f7 k, x
Wilhelm G. Dirks and Hans G. Drexler
9 w% O% P% S( H' k5 p  S4   Classical and Molecular Cytogenetic Analysis. . . . . . . . . . . . . . . . . . . . . . . . . .39' F7 \) s4 G; ]: R$ B
Roderick A.F. MacLeod and Hans G. Drexler2 Y' z" ?6 Q* W/ ?. E" K" k/ p# c
5   Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle . . . . . . . . . . . . 61  x4 G/ ~* A: C0 E
Linda A. Cannizzaro+ r5 E" P' E. V5 c* E
6   The Development of T Lymphocytes in Fetal Thymus Organ Culture . . . . . . .       855 ^* B* @" M' I, O" D
Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama
0 |: _% `2 g* a4 {* K5 a! k% E6 n7  Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors . . . . . . . . . 103
+ F- c* V( ~# Q9 a! D5 ]Génève Awong and Juan Carlos Zúñiga-Pflücker
1 H7 Y% n, ?* u% A5 O: r8   In Vitro Generation of Human T Regulatory Cells: Generation, Culture,and Analysis of FOXP3-Transduced T Cells. . . . . . .115
. u# L  s: Z; f* bAlicia N. McMurchy and Megan K. Levings7 R+ O2 O2 {  A3 |; P9 d
9   Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media. . .    133
; v) T4 I2 ?5 r$ ^( C7 HBert Wognum and Tracy Lee' ]. j+ F9 d( B* o% l/ s) \
10   Isolation and Characterization of Mouse Side Population Cells . . . . . . . . . . . .     151
$ m" ^/ D3 Q2 j; F: TAysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell" N9 K: F* R* K0 G/ T( `
11  Stem Cell Identification by DyeCycle Violet Side Population Analysis . . . . . . .    163
6 q8 s$ R1 v, tWilliam G. Telford
3 V$ b5 N+ p1 C0 t/ x3 C  W12   Isolation and Characterization of Cancer Stem Cells In Vitro  . . . . . . . . . . . . .    1814 r4 U, R) n+ R& r# h/ o1 R
Craig Gedye and Laurie Ailles% v# K3 [" R3 u/ w' Z; G
13  Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets . . . . . . . 205! F5 w1 D1 W" g7 e% c7 \
Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer
2 x! j: x2 c, @/ d# G; M3 U14   Generation and Characterization of Murine Alternatively Activated Macrophages . . . . . . . . . . . .225
: `. R0 I, m. W6 ~Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly2 x2 |, h9 R# P; L$ y' {7 f$ ^) V
15  Human Long-Term Culture Initiating Cell Assay . . . . . . . . . . . . . . . . . . . . . .    241* N# o1 H9 e. ~+ b
Min Liu, Cindy L. Miller, and Connie J. Eaves
# X9 s4 Y, Z0 h, A8 m" F/ m16  Long-Term Culture-Initiating Cell Assay for Mouse Cells . . . . . . . . . . . . . . . .    257! S" J1 ^0 a3 H# ?# b/ @
Stefan Woehrer, Cindy L. Miller, and Connie J. Eaves
" M! G  V2 b4 e* f5 K17  Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells . . . .   267
' e9 [5 f7 Y: |5 {# kBert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller
4 L& @( T* W6 y1 R5 Z: X5 j% r18  Studying Leukocyte Recruitment Under Flow Conditions. . . . . . . . . . . . . . . .    285+ b6 o# M2 k0 w5 g
Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel1 B: X) b# r; n8 S$ F$ E8 y
19   Generation and Establishment of Murine Adherent Cell Lines. . . . . . . . . . . . .     301
+ r- w' e' x. Q) r" z1 LRouzanna Istvanffy and Robert A.J. Oostendorp
, q2 f  V0 o4 h( B8 O20   Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture . . . . . . 3159 K- Q+ L" g( ~, ~! j3 [
Ravenska Wagey and Brenton Short
9 ?! c' E9 J7 w5 q: ]21   Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone . . . . . . . . . . . .335
$ D3 O7 A3 o9 }; ]% IBrenton Short and Ravenska Wagey4 _2 c1 `- k- M
22   Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture . . . . . . . . . . .349* p. T$ ~+ ?. U
Katharina Schallmoser and Dirk Strunk
4 F8 R( e  K/ y' `& n23  In Vitro Methods to Culture Primary Human Breast Epithelial Cells. . . . . . . .    3633 N( _( t5 X. i% X
Afshin Raouf and Yu Jia Sun. n( a) {. Q) R0 Y3 O) J9 S5 J
24  Human Prostate Epithelial Cell Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3835 @# G, X) T5 G. y
Johng S. Rhim
% L' M+ t& d5 F6 ?+ ^' s25  Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue . . . . . . . .395' A& q# @8 K1 u/ g" a# |/ o
Michael Prater, Mona Shehata, Christine J. Watson, and John Stingl
" p0 T6 Z7 u4 ?- L$ \/ H2 J26   Isolation and Characterization of Human Hair Follicle Epithelial Cells . . . . . .     411
; R+ @0 j' M0 QKeita Inoue and Kotaro Yoshimura
7 ~) d" O  w' @  k; R27   Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells . . . . .4235 Q( w5 f" b% z2 n8 N% ^# {/ P! E
Ricarda Glaum and Margit Wiedmann-Al-Ahmad
3 U/ K' E4 k3 w0 u8 J28  Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells . . . . . . 431! L+ K/ e- \. Z2 C8 ~# K
Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni
5 b) c7 t' [$ r' V3 m29  Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cell ............469
: d) T3 b% C# U& R2 RTakamichi Ishii, Kentaro Yasuchika, and Iwao Ikai
& J  }, w9 H- H8 [$ N30   Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System . . . 479
/ W3 V" F. R9 `7 H; [Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds
0 z; r5 L! `1 I, `" g+ c31  Feeder-Independent Culture Systems for Human Pluripotent Stem Cells. . . . .    507
. H1 {* V& T2 }; J$ WJennifer Moody
5 [& G& ~3 z; h" o32   Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates . . . . . . . . 5235 \4 i5 G3 q( M* C
Jennifer Antonchuk
1 l% D) [4 R4 E1 r# t: S& h33   Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells. . . . . . . . . . . . . . . . . . .5358 m# j) x6 v, H1 r6 F6 F$ W7 N
Nirupama K. Shevde and Amber A. Mael) `) Z4 J5 B  l/ |% K
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547
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pailishi

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