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- 积分
- 266
- 威望
- 266
- 包包
- 863
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O) J( P# C8 C2 O Q/ b4 k
(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g
$ I( t) S N5 e) r) b( EMgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),! ^( o' g- n. S3 X3 ]3 K! N
4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;
6 E( K; Y1 @) x8 W3 u7 a9 @Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.
5 R* J& R0 S PG6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g
5 [( M/ v; O% e! m6 A1 rpenicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate
+ |& C, H: I: e1 h/ [(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of
. C( H! T }, R+ {0 r: C# k$ v6 t# a! KMilli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L% g9 J) h; R/ O0 }5 W5 z
with Milli-Q water. |
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总评分: 威望 + 10
包包 + 10
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发表于 2009-9-21 09:25
发表于 2009-9-26 07:48
