M2培养液

POPOLD

M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O
( T) k/ s$ B- h% _# \0 H! w4 D(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g& C' \* d$ v2 W; `1 U
MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),0 a" h( c+ D( {0 T) T
4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;. H0 _3 w( D2 _
Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.
  r7 e" c3 x  v& zG6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g- @, V5 F) P2 c( c+ X% H# g3 Q
penicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate
% h% ]: L: [4 m3 W7 A2 o( _(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of
, R. W$ u8 ]3 Y. {Milli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L
* W5 H/ r1 U. x. H' Twith Milli-Q water.

zpzp0312

谢谢！~~~

quixote1983

还有编号，太职业了

POPOLD

您的 满意 ，是版主最大的希望，还可亲临指导

jayhaoli01

谢谢！

hualin840518

回复 1# POPOLD ( v. z2 [% V& O+ i5 @8 f

7 r8 W6 K/ Q: u9 V  }( F1 x% v
$ v: ]) _, c7 X% V6 N4 q4 Z$ r    来源是那篇文章
; @5 [. f4 F& k) I6 n, f谢谢！

edisonlou

谢谢

llingzu

谢谢

基拉

谢谢

POPOLD

回复 12# hualin840518 $ ~% {1 s7 M- a7 A- ?3 @

. S) s7 J, }2 p8 g* _
  o4 ]) S. [& L8 ]' r    yige 一个牛核移植的protocol