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- 积分
- 264
- 威望
- 264
- 包包
- 858
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O" v7 |7 A) ?6 b% ^) j6 V4 ]1 p2 u
(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g
|4 p/ P& b/ \- DMgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),
: `) @1 } Z6 D6 T4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;
z9 \: Q, N7 E, o; b' ]Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.
) n c, W. p9 h' d/ @( `4 v/ g0 uG6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g) r. ?, \' i9 E' V" ?1 n
penicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate
5 M7 s! t( A& q(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of
' V" ^2 A: y6 E7 rMilli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L) J8 z3 D3 b: z- ~5 V( \- m& Z& I. g
with Milli-Q water. |
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总评分: 威望 + 10
包包 + 10
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发表于 2009-9-21 09:25
