Pericentrin pivotal in primary cilia

杨柳

The centrosome protein pericentrin acts as an anchor for primary cilia formation in human cells, according to a report by Jurczyk et al. (page 637). The finding further unites the centrosome and primary cilia, both of which are based around centrioles, and both of which use pericentrin as an anchor for other proteins.& m' g% v  W8 X' w" t% R' b' r
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The authors show that pericentrin is located not only at centrosomes but also around the centrioles at the base of primary and motile cilia. After depletion of most pericentrin using RNAi, primary cilia failed to assemble, but centrosomes could still nucleate microtubules. The divergent outcomes may result from selective depletion of pericentrin's large isoform by RNAi, with remaining smaller isoforms perhaps acting in centrosomes.2 a2 N" ^3 H7 I0 J" b

% X) ^1 ~! y0 ^1 Q+ I) ^Depletion of pericentrin also disrupted the localization of both the intraflagellar transport (IFT) proteins that help build cilia and PC2, a cation channel important in ciliary signaling. Furthermore, depletion of IFT proteins with RNAi caused pericentrin mislocalization and disrupted primary cilia formation.
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( P5 n) p1 b1 ^& a" X) wEndogenous pericentrin copurifies and coimmunoprecipitates with IFT proteins. Additional studies are needed to understand exactly how this complex acts to direct primary cilia formation. The association is certainly reminiscent of pericentrin's function in centrosomes, where it is needed to anchor the -tubulin ring complexes that nucleate microtubules.(Pericentrin (green) is needed to build p)

昕昕

只有一条路不能选择——那就是放弃的路；只有一条路不能拒绝——那就是成长的路。  

s06806

原来这样也可以  

泡泡鱼

谁都不容易啊 ~~  

昕昕

顶也～  

tuanzi

顶的就是你  

红旗

每天早上起床都要看一遍“福布斯”富翁排行榜，如果上面没有我的名字，我就去上班……  

龙水生

呵呵 大家好奇嘛 来观看下~~~~  

陈晴

谢谢楼主啊！

sky蓝

青春就像卫生纸。看着挺多的，用着用着就不够了。