PDF电子书：Basic Cell Culture Protocols (Springer MMB-Vol946, 4e, 2013)

xyklmu

- `: C+ k* t, s2 c4 `& D
) G! D" Y! o* s3 ~
[hide][/hide]
( v' b, G. K: r3 |/ o
2 R. n8 P. L6 {- e  m0 x/ g/ [+ m+ P
% ^  z' {' G0 @/ B2 h: M0 n
Methods in Molecular Biology
* c* e6 Y  w; Q! j# hVolume 946 20137 j6 A% R- T3 a
Basic Cell Culture Protocols/ n2 T3 n+ v# }6 H1 L
Editors:
6 b  ?! |0 T, t4 ]% K( _    Cheryl D. Helgason,
1 k) e" ^1 b, J+ G! E8 V  V5 T    Cindy L. Miller
+ F( O% n* p- f  K/ O  z6 `4 n; @. F* h+ ^
ISBN: 978-1-62703-127-1 (Print) 978-1-62703-128-8 (Online)
7 L# A4 I1 Y% Y# N' ZTable of contents (33 chapters)
6 |7 c" R' M: |; \) \Contents
; Q. x2 q  I. Y) _, [Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
' E6 W) `% T( i  T! eContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
( z" h/ }. L+ a$ H# I1 Detection of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . . 1, C" ?0 x) k- P% i4 e# V. w
Cord C. Uphoff and Hans G. Drexler
$ k/ Q7 g( W" ^6 s+ R2 Eradication of Mycoplasma Contaminations . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3 R# l+ H7 _. Q" Y: bCord C. Uphoff and Hans G. Drexler
6 {" T/ J4 L1 I: n3 STR DNA Typing of Human Cell Lines: Detection of Intraand Interspecies Cross-Contamination and Interspecies Cross-Contamination  27+ u4 v) i2 {' S, s
Wilhelm G. Dirks and Hans G. Drexler. S" t* q) M3 a1 C2 M# c
4   Classical and Molecular Cytogenetic Analysis. . . . . . . . . . . . . . . . . . . . . . . . . .39
% L1 a1 F5 ^7 K& WRoderick A.F. MacLeod and Hans G. Drexler
/ [+ `1 ^  p/ ~( a6 Z7 y6 ^5   Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle . . . . . . . . . . . . 61/ l/ |$ d9 E. d8 x* n
Linda A. Cannizzaro( ?: ^7 V8 v- Q( ~
6   The Development of T Lymphocytes in Fetal Thymus Organ Culture . . . . . . .       854 h, C+ k7 s- d% R* P2 L8 H% t
Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama! |! F5 V+ s4 B7 T; K- `2 b) D
7  Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors . . . . . . . . . 103
0 q" R4 D; W  |) H: h$ e1 u8 w; HGénève Awong and Juan Carlos Zúñiga-Pflücker/ V; T% B+ |2 l! t7 `
8   In Vitro Generation of Human T Regulatory Cells: Generation, Culture,and Analysis of FOXP3-Transduced T Cells. . . . . . .115! e8 B+ w+ T( q  I
Alicia N. McMurchy and Megan K. Levings
& s3 b4 q( d/ T9 f9   Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media. . .    133+ `' f1 Z" d+ \( Z- N
Bert Wognum and Tracy Lee6 Y! c" b: I& B7 n/ Q4 G1 _4 w9 d
10   Isolation and Characterization of Mouse Side Population Cells . . . . . . . . . . . .     151
- L( D9 \) B9 I5 b1 |4 IAysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, and Margaret A. Goodell4 `% [* O1 f3 `4 Z' s  [0 h
11  Stem Cell Identification by DyeCycle Violet Side Population Analysis . . . . . . .    163
6 Z$ m" H! q, _- Z8 p; F/ k# gWilliam G. Telford
! f7 e2 H& d& c. _12   Isolation and Characterization of Cancer Stem Cells In Vitro  . . . . . . . . . . . . .    181
4 c3 C" p" w+ f9 ?1 k* }) jCraig Gedye and Laurie Ailles( w5 J1 s6 Y# E2 p, ~9 B1 D% N6 A
13  Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets . . . . . . . 205: K. a2 G1 J2 q+ j- _2 Y$ a
Nicolas Pineault, Amélie Robert, Valérie Cortin, and Lucie Boyer& H! F* A9 ~! Y+ B3 r
14   Generation and Characterization of Murine Alternatively Activated Macrophages . . . . . . . . . . . .225& u+ ]$ W* L8 O! t/ k
Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, and Laura M. Sly
' a5 F' g2 ^. \! w. S+ O15  Human Long-Term Culture Initiating Cell Assay . . . . . . . . . . . . . . . . . . . . . .    241
0 P4 |5 f7 [) ~$ }& {. P) eMin Liu, Cindy L. Miller, and Connie J. Eaves
/ ~7 I  }) p! e6 C16  Long-Term Culture-Initiating Cell Assay for Mouse Cells . . . . . . . . . . . . . . . .    257
2 O0 q- T+ l8 y$ {# j) IStefan Woehrer, Cindy L. Miller, and Connie J. Eaves% {- v. u1 f+ R' t% e4 e. o
17  Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells . . . .   267, O, y" V1 m5 ]8 `
Bert Wognum, Ning Yuan, Becky Lai, and Cindy L. Miller
8 `. ~0 O5 G0 _) V1 w8 k( `18  Studying Leukocyte Recruitment Under Flow Conditions. . . . . . . . . . . . . . . .    285
$ H0 v) N$ T: z, M) ]Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, and Kamala D. Patel( C( |+ Y3 Q# G" A
19   Generation and Establishment of Murine Adherent Cell Lines. . . . . . . . . . . . .     301
; x" a" D. g- s/ Y' X/ K+ fRouzanna Istvanffy and Robert A.J. Oostendorp6 u3 q/ ]! h; q1 ?
20   Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture . . . . . . 3158 u& V, H+ ^! |/ F
Ravenska Wagey and Brenton Short
' d3 a, ?, P/ a' l9 e+ ^2 k6 g21   Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone . . . . . . . . . . . .335
$ a( ]4 J/ b  x, U. n' GBrenton Short and Ravenska Wagey7 c. A5 g4 g: G9 m" ~9 N* x
22   Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture . . . . . . . . . . .349) A3 W& c' J) ]* y% q
Katharina Schallmoser and Dirk Strunk7 w# O$ p, l  s$ {3 v2 p: O6 B
23  In Vitro Methods to Culture Primary Human Breast Epithelial Cells. . . . . . . .    363
' y. M3 U/ f. e0 ~1 M7 WAfshin Raouf and Yu Jia Sun6 o* l& e6 r( V6 T$ F1 T
24  Human Prostate Epithelial Cell Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . 383/ d2 d3 M3 }7 O, t5 g' h2 A7 p
Johng S. Rhim
6 C% f4 u0 P! f% v25  Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue . . . . . . . .395
& W2 F+ U. H9 R2 S: H6 a( SMichael Prater, Mona Shehata, Christine J. Watson, and John Stingl
! i* Y5 c6 |9 c5 V/ Y/ h26   Isolation and Characterization of Human Hair Follicle Epithelial Cells . . . . . .     411
" d( b) T0 ~' @Keita Inoue and Kotaro Yoshimura3 y9 K  t3 j* o; g1 A# p
27   Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells . . . . .423. o& g! p$ Q" u
Ricarda Glaum and Margit Wiedmann-Al-Ahmad
3 F; z* \6 o' y- B* |/ l28  Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells . . . . . . 431" V" q+ G# e( t1 @$ H! H
Paul Keire, Andrew Shearer, Gabi Shefer, and Zipora Yablonka-Reuveni
1 t  C, f* w, D* d) e' f29  Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cell ............4692 N# B3 c/ a2 W9 Z0 I: h
Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai
2 V: m6 {/ D. ]: I3 {6 F& W% h. G30   Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System . . . 479) ?, t2 g1 p! u8 b2 ~4 J# F
Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds
$ b* V0 S& o0 Y) E6 Q* U31  Feeder-Independent Culture Systems for Human Pluripotent Stem Cells. . . . .    5076 c) e% t! }4 o7 p! F5 g" d
Jennifer Moody
& d! {: O2 k- ]+ O# S: z/ K32   Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates . . . . . . . . 5237 X  g0 W+ v7 ~- S3 y) l- z3 E
Jennifer Antonchuk, W" o# L* \1 x# B% u
33   Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells. . . . . . . . . . . . . . . . . . .535! J; s$ ~% A* o& w3 r; z) w) x) Q
Nirupama K. Shevde and Amber A. Mael
& Z1 b  q0 b7 p. ~$ g0 eIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5470 L7 s6 k$ e2 Q8 u9 W* A. Z
+ f, ]  a4 A3 }$ F# M

" B) j/ A6 l, _; r. \+ W
' l, {" P, y+ I6 x$ l% s9 P

pailishi

谢谢共享

peiguos

怎么看不到？

wujh1986

谢谢

henryjia

gooooooood

clairezy1983

好书求下载~~~~

clairezy1983

只有1-24章，缺后面的部分，楼主能补齐吗？

lixin030287

good

lixin030287

good

franklinhg

Thanks  for sharing