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本帖最后由 细胞海洋 于 2011-9-1 22:04 编辑 9 m( I3 ~1 }. d2 U0 c, P
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5 ~/ _& r$ _* R7 pFundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition
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8 t$ L3 K; a- J& FIn response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.- f+ q" [% O- K. i& ~& m) ^
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Chapters Title
% V3 {7 `+ O4 j( c: i; C1 Introduction' v) `1 ^7 y/ }3 M8 O8 C A
2 Design and Equipment for the Cell Culture Laboratory* D/ P( v5 O8 i1 V
3 Safety Aspects of Cell Culture! g+ Z$ {* @6 G9 C8 G
4 Sourcing of Cell Lines3 C5 j# b$ ^7 I& {$ I1 r) t
5 Cell Types & Culture Characteristics
+ L7 r" e) N M3 X9 I. x6 The Cell Environment (including types of culture medium)$ w" h- R4 z6 s
7 Cryopreservation and Storage of Cells
9 d9 F. b Y4 c0 g0 v$ U. ~- P8 Good Cell Banking Practices
) A; U/ _ H. b; V3 w9 Quality Control Considerations in Cell Culture4 `, H) K6 V2 |5 G7 L! W1 L
10 Authentication of Cell Lines5 `# d2 l5 h U, F5 S
11 Alternative Culture Systems
" A% [7 B7 {* @) W7 I12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture
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# C; @ E% F: R" K) S I- E' sProtocols Title2 \( v# W: V9 d- n1 ~
1 Aseptic Technique and Good Cell Culture Practice
( f6 Y0 ^$ p- O' v V% j* J+ W2 Resuscitation of Frozen Cell Lines* L' Z5 I: Y. S$ x9 Q
3 Subculture of Adherent Cell Lines
6 p9 O$ G# G" y* K4 Subculture of Semi-Adherent Cell Lines! A3 Y) x+ c$ e2 k4 c$ ~
5 Subculture of Suspension Cell Lines6 n Y0 h, U/ }3 r7 D, U7 b! @
6 Cell Quantification- B2 D2 S; b, J, ]* i: W2 f* }
7 Cryopreservation of Cell Lines
( W- c) O2 Y0 `" Y5 a. k0 w8 Testing for Bacteria and Fungi in Cell Cultures* L5 m9 G7 P# g3 s/ M5 X2 N
9 Testing for Mycoplasma by Culture Isolation& G9 D5 ^4 E3 Q4 C# t6 i% X
10 Testing for Mycoplasma by Indirect DNA Stain& M: D) R3 D; r: H
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Glossary of Terms; }! G8 F$ ]$ h, r
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发表于 2011-9-1 15:17
