有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章7 Q$ B  n+ Q# q& z; n& r* x5 ~, `. I5 F
2002 Lactate inhibits germ cell apoptosis in the human testis
: x  h% I6 ~! J* o! A2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture$ J, h% H  O1 \7 {$ R
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal% b3 q' R9 s" I1 i9 u" F' G
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
3 y. O2 _" V  b  r" |! c2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro
' l  _; m% I' h; U4 q4 A2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture  P: k7 B; j3 M4 b0 R% w$ E0 x, W+ h
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis
& U. l# P0 H) x5 g2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
+ X% j6 X2 Q3 O* t* d8 E: Z2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse
! }0 p2 n1 C) R0 l2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines
7 d$ n0 t/ N) d. {" Q2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
% U: j8 |# Y0 z4 @! U6 w, h( u2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells
  g! S# h" g# g! A) @9 C/ r2005 Anchorage-independent growth of mouse male germline stem cells in vitro
9 t3 y, G8 h; x+ `2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor
) D4 M# A0 a  p$ u) h% s: J2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat" \, b) K. v7 w( b  r
2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment4 t! ^+ ~% {$ N9 J/ X
2005 Isolation of male germ-line stem cells; influence of GDNF. ~. f. h1 ?6 m' {
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions7 I2 d6 M' E, h8 n
2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture  a: v* m6 ~3 |6 d
2006 In vitro propagation of spermatogonial stem cells from KM mice! u8 |$ B! r3 z/ o% K" a
0 D% i- ^1 D/ J: X4 r1 l5 U- V# y
上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的