有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章$ R; |. y$ d- I- K3 w. J; A" A
2002 Lactate inhibits germ cell apoptosis in the human testis+ k' ~# t1 p/ j1 `2 u5 v$ t$ n
2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture/ M3 e" s( O; @- ^* c9 t
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal
# u; L( t7 V2 O' q2 z2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
# w1 c# I( B- k2 H. p2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro& W0 U# }* R9 `4 m! g, W1 T2 \
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture: U& w: w, x& Y& b1 V! E8 e& |
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis3 I! S* K) t+ w: Q" W
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
3 ]* L+ i3 b7 k' H2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse' w# ~" _( @: D' ~& j- Z
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines9 E+ N( }/ T; q2 N$ J- y9 D. R. H
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells  I: n% `8 X0 Q6 x2 S% e
2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells- V( N' T0 A2 h; \' V! e5 H7 W
2005 Anchorage-independent growth of mouse male germline stem cells in vitro+ V$ {, N  Z' E% |  h; g, E! v* g9 L) U
2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor+ F. D; G. _7 U3 K. Y- t
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat$ z" j2 b; n- u3 e% ~
2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment$ i3 i( h3 s5 Q+ X( p/ V) X
2005 Isolation of male germ-line stem cells; influence of GDNF
3 ~1 s9 |  c% ?# m; b1 _  H: m2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions6 e0 a1 J' d1 N- o
2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
1 U# q8 ~3 d# i" ]" _2006 In vitro propagation of spermatogonial stem cells from KM mice
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) P) r4 g$ `5 G) \& ^+ [5 Z上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的