有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章# c- I* l% w' ]& U0 T
2002 Lactate inhibits germ cell apoptosis in the human testis
0 X; u0 [. ?' l: u5 ]' ~2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture& q/ `# T! q" M2 |0 I6 ^
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal
- I, Z2 `3 _8 k2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
4 H5 N$ R/ \3 y2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro# j% J7 {2 I5 _9 t( K9 M
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture
% Y8 X# `( P& t5 i6 P0 @2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis
. d5 M# i. m; G( A2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture' c% `$ o, _) M2 I4 W# u9 Z+ f
2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse$ b$ j) y1 o: a$ o' X
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines
. Q( a- V, V2 k* c  `; q6 L9 |. D( v* r2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
4 x8 q5 V. b+ \! @' X7 Z7 T2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells
% [/ z1 Y# o- Z* h# W0 i; j2005 Anchorage-independent growth of mouse male germline stem cells in vitro
0 p/ V, g) |9 B. W: e/ a7 |5 C2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor
. P5 E0 Q: p' ~* p1 V$ H1 C2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
* x3 ^* y3 i/ v) y4 i2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
) l! |, J( @2 c; A; C7 ^! w2005 Isolation of male germ-line stem cells; influence of GDNF+ c" e( C" ?8 P# _) S
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
% h# i0 D& {! A- j" V2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
, G8 i9 p1 t3 `; g; O2006 In vitro propagation of spermatogonial stem cells from KM mice
: Q  Q/ }6 M- h- R: ^$ T; d8 g' k0 w% Y0 a, f3 W# |% g2 x
上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的